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Species: Saccharomyces cerevisiae
Genetic Insert: acs1*
Vector Backbone Description: Backbone Marker:Liu Yiqi; Backbone Size:6469; Vector Backbone:pPIC 3.5K(DHis4); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:31077813
Comments: Dep. confirms the following mutations A30S, P113S, and S629N do not affect function.
Proper citation: RRID:Addgene_126736 Copy
Species: Aspergillus terreus, Aspergillus nidulans
Genetic Insert: atX,npgA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:9004; Vector Backbone:pPIC3.5K; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:31077813
Proper citation: RRID:Addgene_126725 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4500; Vector Backbone:pGL4.23; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27708057
Comments: Use FspI and AclI to remove the kanamycin resistance cassette and replace with putative regulatory elements by Gibson cloning.
Proper citation: RRID:Addgene_86461 Copy
Genetic Insert: 3xFLAG
Vector Backbone Description: Vector Backbone:pFA6a-kanMX6; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27825338
Proper citation: RRID:Addgene_78383 Copy
Genetic Insert: 3xPK
Vector Backbone Description: Vector Backbone:pFA6a-kanMX6; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27825338
Proper citation: RRID:Addgene_78382 Copy
Genetic Insert: 13xMyc
Vector Backbone Description: Vector Backbone:pFA6a-kanMX6; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27825338
Proper citation: RRID:Addgene_78385 Copy
Genetic Insert: 3xHA
Vector Backbone Description: Vector Backbone:pFA6a-kanMX6; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27825338
Proper citation: RRID:Addgene_78384 Copy
Species: SV40, Entacmaea quadricolor
Genetic Insert: nuclear localization signal (nls) tagRFP
Vector Backbone Description: Backbone Size:9530; Vector Backbone:pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27775045
Comments: Backbone plasmid (pK038) is available in Addgene.
Proper citation: RRID:Addgene_85179 Copy
Species: Synthetic
Genetic Insert: 3xFLAG-piggyBac-DsRed
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR™4-TOPO; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin
Proper citation: RRID:Addgene_80820 Copy
Species: Caenorhabditis elegans
Genetic Insert: sls-1.2
Vector Backbone Description: Backbone Marker:Transgen; Backbone Size:3830; Vector Backbone:pEASY-Blunt Simple Cloning Vector; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27557708
Proper citation: RRID:Addgene_81114 Copy
Vector Backbone Description: Backbone Size:5127; Vector Backbone:pONSY; Vector Types:Capsaspora owczarzaki; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:29752387
Proper citation: RRID:Addgene_111873 Copy
Genetic Insert: Lifeact fused to mCherry
Vector Backbone Description: Backbone Size:5127; Vector Backbone:pONSY; Vector Types:Capsaspora owczarzaki; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:29752387
Proper citation: RRID:Addgene_111876 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:5127; Vector Backbone:pONSY; Vector Types:Capsaspora owczarzaki; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:29752387
Proper citation: RRID:Addgene_111874 Copy
Species: Capsaspora owczarzaki
Genetic Insert: Capsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
Vector Backbone Description: Backbone Size:5127; Vector Backbone:pONSY; Vector Types:Capsaspora owczarzaki; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:29752387
Proper citation: RRID:Addgene_111878 Copy
Species: Soybean
Genetic Insert: APEX2
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:3267; Vector Backbone:pKD4; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:30275513
Proper citation: RRID:Addgene_112868 Copy
Species: Desulfovibrio vulgaris
Genetic Insert: uracil phosphoribosyltransferase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCR4/TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23393089
Comments: Selection for kanamycin resistance after introduction of pMO746 identifies those cells in which integration into the chromosome of part or all of the plasmid has occurred.
Kanamycin resistance is a strong selection in the sulfate-reducing bacteria (SRB). Ampicillin is not universally effective in the SRB but is often used for selection in Escherichia coli. Thus this plasmid can be readily grown in E. coli but is actually unstable in the SRB.
Proper citation: RRID:Addgene_117480 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191617 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191619 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191618 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191624 Copy
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