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Species: Saccharomyces cerevisiae
Genetic Insert: SUP45 gene encoding translation release factor 1 from S. cerevisiae
Vector Backbone Description: Backbone Size:5976; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20444877
Proper citation: RRID:Addgene_29376 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUP45 gene encoding translation release factor 1 from S. cerevisiae
Vector Backbone Description: Backbone Size:5976; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20444877
Proper citation: RRID:Addgene_29373 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUP45 gene encoding translation release factor 1 from S. cerevisiae
Vector Backbone Description: Backbone Size:5976; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20444877
Proper citation: RRID:Addgene_29394 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUP45 gene encoding translation release factor 1 from S. cerevisiae
Vector Backbone Description: Backbone Size:5976; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20444877
Proper citation: RRID:Addgene_29388 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUP45 gene encoding translation release factor 1 from S. cerevisiae
Vector Backbone Description: Backbone Size:5976; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20444877
Proper citation: RRID:Addgene_29387 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUP45 gene encoding translation release factor 1 from S. cerevisiae
Vector Backbone Description: Backbone Size:5976; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20444877
Proper citation: RRID:Addgene_29384 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Uba1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5500; Vector Backbone:pBacPAK8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10973499
Proper citation: RRID:Addgene_29508 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ePDZb1–mCherry
Vector Backbone Description: Vector Backbone:YIPlac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388287
Proper citation: RRID:Addgene_34981 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ePDZb–mCherry
Vector Backbone Description: Vector Backbone:YIPlac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388287
Proper citation: RRID:Addgene_34980 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ePDZb
Vector Backbone Description: Vector Backbone:YIPlac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388287
Proper citation: RRID:Addgene_34984 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mid2(SS/TM)-GFP-LOVpep
Vector Backbone Description: Backbone Size:4263; Vector Backbone:YIPlac128; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388287
Comments: The LOV2 domain is amino acids 404-540 and differs from the wild-type A. sativa LOV2 sequence by the point mutations G528A and N534E. See Strickland et al. for details.
Proper citation: RRID:Addgene_34971 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mid2(SS/TM)-GFP-LOVpep
Vector Backbone Description: Backbone Size:4263; Vector Backbone:YIPlac128; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388287
Comments: The LOV2 domain is amino acids 404-540 and differs from the wild-type A. sativa LOV2 sequence by the point mutations G528A and N534E. See Strickland et al. for details.
Proper citation: RRID:Addgene_34970 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ePDZb
Vector Backbone Description: Backbone Marker:EUROSCARF; Backbone Size:6624; Vector Backbone:pGREG503; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388287
Proper citation: RRID:Addgene_34978 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA
Vector Backbone Description: Vector Backbone:pBbA5c; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21952217
Comments: Please note that the R364 in Mevalonate Kinase (MK) may be susceptible to mutations. Although the original plasmid encoded R364C, Addgene's stock contains R364S. This mutation is not expected to impact overall function, however the exact effects are unknown.
Proper citation: RRID:Addgene_35152 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: lys5MX3
Vector Backbone Description: Vector Backbone:pFA6-kanMX4; Vector Types:Yeast Replicating; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14745782
Proper citation: RRID:Addgene_35112 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: lys5MX4
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast replicating; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14745782
Proper citation: RRID:Addgene_35116 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: fcy1PR
Vector Backbone Description: Vector Backbone:pFA6-kanMX3; Vector Types:Yeast replicating; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16088873
Proper citation: RRID:Addgene_35104 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: fcy1MX4
Vector Backbone Description: Vector Backbone:pFA6-kanMX4; Vector Types:Yeast replicating; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16088873
Proper citation: RRID:Addgene_35102 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: loxP KAN loxP 24xPP7SL MDN1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21512033
Comments: This plasmid was designed to construct mRNAs that contain multiple copies of an RNA sequence (the 24xPP7 stem-loops) that can be recognized by a chimeric RNA-binding protein fused to GFP, encoded in plasmid pDZ276 (Addgene plasmid #35194). See associated publication for detailed methods and use of this plasmid.
Please note that the LacZa-ccdB fusion in this plasmid is not functional and was originally present in the vector backbone to aid in screening for insert.
Addgene's sequencing results identified a 3 nucleotide deletion at bp# 543-545 when compared to the full plasmid sequence provided by the depositing laboratory. This deletion is not known to affect plasmid function.
Proper citation: RRID:Addgene_35191 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 2xPH(Osh2) fusion
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21295699
Comments: Please note that the first Osh2 domain contains aa273-421 and the second Osh2 domains contains aa260-421 when compared to GenBank reference sequence NP_010265.1. According to this reference sequence the PH domain is aa 292–384. The additional residues surrounding the PH domain were intentionally cloned into this construct.
Proper citation: RRID:Addgene_36095 Copy
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