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Vector Backbone Description: Vector Backbone:pLTE EGFP; Vector Types:Cell-free expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25529348
Comments: attR1-ccdB-CamR-attR2-eGFP-8xHis-Stop
Proper citation: RRID:Addgene_67138 Copy
Vector Backbone Description: Vector Backbone:pLTE EGFP; Vector Types:Cell-free expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25529348
Comments: attR1-ccdB-CamR-attR2-mCheery-8xHis-Myc-Stop
Proper citation: RRID:Addgene_67142 Copy
Vector Backbone Description: Vector Backbone:pCellFree_G08; Vector Types:Cell-free expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25529348
Comments: sfGFP-8xHis
Proper citation: RRID:Addgene_67144 Copy
Vector Backbone Description: Backbone Marker:Addgene Yeast Gateway collection (Lindquist / Gitler); Backbone Size:7574; Vector Backbone:pRS424-GPD-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29212664
Proper citation: RRID:Addgene_70124 Copy
Species: Mus musculus
Genetic Insert: Rosa26 5-homology region
Vector Backbone Description: Vector Backbone:Bluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26772810
Proper citation: RRID:Addgene_74284 Copy
Genetic Insert: Gateway cassette
Vector Backbone Description: Vector Backbone:pUC13T DNA; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30504365
Comments: bioRxiv doi.org/10.1101/445833
Proper citation: RRID:Addgene_74756 Copy
Species: Neurospora crassa
Genetic Insert: QS
Vector Backbone Description: Vector Backbone:pUCTCAPR3-GW; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30504365
Comments: bioRxiv doi.org/10.1101/445833
Proper citation: RRID:Addgene_74757 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway cassette
Vector Backbone Description: Vector Backbone:pUCTCAPR3-GW; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30504365
Comments: bioRxiv doi.org/10.1101/445833
Proper citation: RRID:Addgene_74747 Copy
Species: Other
Genetic Insert: Cm(R)-CcdB
Vector Backbone Description: Backbone Size:2000; Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27088723
Comments: Want all of the CRISPRainbow constructs? Request the set here: https://www.addgene.org/kits/pederson-crisprainbow/
Proper citation: RRID:Addgene_75398 Copy
Vector Backbone Description: Backbone Size:4181; Vector Backbone:PCS2+ was modified to generate pCSf107mT; Vector Types:Mammalian Expression, in vitro transcription or translation off of an SP6 promoter, Xenopus expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26391338
Proper citation: RRID:Addgene_67616 Copy
Vector Backbone Description: Backbone Size:4181; Vector Backbone:PCS2+ was modified to generate pCSf107mT; Vector Types:Mammalian Expression, in vitro transcription or translation off of an SP6 promoter, Xenopus expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26391338
Proper citation: RRID:Addgene_67617 Copy
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27621059
Proper citation: RRID:Addgene_67691 Copy
Species: Gallus gallus
Genetic Insert: actin prom-dsRed-scramble shRNA
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pUC; Vector Types:Mammalian Expression, AAV, RNAi; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25706476
Comments: To express shRNAs, we used the pPRIME system. This generates
micro-RNA-30 (mir30)-derived shRNAs and co-expression of a marker protein, such as dsRED, from the same transcript. The shRNA hairpin sequences were placed into the mir30 site of pPRIME-cmv-dsRed-FF3 and then cloned into the AAV vector (Zhang et al 2015 Nat Neurosci),
SbfI(or PstI) + PacI will retrieve the 2.1kb insert (dsRed-scramble shRNA-chloramphenicol) and the 5.1kb AAV-CBA backbone, which is ampicillin resistant. The SbfI site in the middle of dsRed has been silenced. The construct can be linearised with XhoI.
Proper citation: RRID:Addgene_67879 Copy
Species: Gallus gallus
Genetic Insert: dsRed and shRNA to knock down the mouse adra2 receptor
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pUC; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25706476
Comments: The shRNA hairpin sequences were placed into the mir30 site of pPRIME-cmv-dsRed-FF3 (Addgene Plasmid 11664; gift from S. Elledge, Howard Hughes Medical Institute). Inserts in pPRIME were released by SbfI and PacI digestion, and cloned into the PstI and PacI sites in the polylinker of the AAV-genome plasmid pAM-flex (Murray AJ et al.2011 Nat Neurosci 14, p297), to give ITR-cmv-Penhancer/chicken β-actin-dsRED-mir30-shRNA-woodchuck post-translational regulatory sequence (WPRE)-bovine growth hormone polyadenylation signal (pA)-ITR. The sh RNA knocks down mouse adra2a expression.
Proper citation: RRID:Addgene_67880 Copy
Vector Backbone Description: Backbone Marker:Austin Smith, University of Cambridge; Backbone Size:8563; Vector Backbone:AGS644 pCAGS-IRES-Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25915022
Comments: Note: Addgene's quality control sequencing identified a Y64H amino acid residue substitution in the Hygromycin resistance cassette. This residue change is not thought to affect the plasmids function or ability to confer resistance to Hygromycin.
Proper citation: RRID:Addgene_68265 Copy
Vector Backbone Description: Backbone Marker:Invitrogen ; Backbone Size:10200; Vector Backbone:pCEP4; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27528826
Proper citation: RRID:Addgene_68342 Copy
Genetic Insert: p65AD::CaM
Vector Backbone Description: Backbone Marker:see publication for details; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25961791
Proper citation: RRID:Addgene_64719 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2850; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24737862
Proper citation: RRID:Addgene_65617 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2850; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24737862
Proper citation: RRID:Addgene_65502 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2850; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24737862
Proper citation: RRID:Addgene_65505 Copy
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