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Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24324157
Comments: This destination vector for TALEs is compatible with the Zhang Lab TALE Toolbox.
Proper citation: RRID:Addgene_49631 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24324157
Comments: This destination vector for TALEs is compatible with the Zhang Lab TALE Toolbox.
Proper citation: RRID:Addgene_49630 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24324157
Comments: This destination vector for TALEs is compatible with the Zhang Lab TALE Toolbox.
Proper citation: RRID:Addgene_49624 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24324157
Comments: This destination vector for TALEs is compatible with the Zhang Lab TALE Toolbox.
Proper citation: RRID:Addgene_49626 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24324157
Comments: This destination vector for TALEs is compatible with the Zhang Lab TALE Toolbox.
Proper citation: RRID:Addgene_49625 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2400; Vector Backbone:pDESTR4-R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24820376
Comments: See www.wormbuilder.org for updated protocols.
Proper citation: RRID:Addgene_51481 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2400; Vector Backbone:pDESTR4-R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24820376
Comments: See www.wormbuilder.org for updated protocols.
Proper citation: RRID:Addgene_51483 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23328393
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.
Proper citation: RRID:Addgene_71500 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.
Proper citation: RRID:Addgene_71501 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.
Proper citation: RRID:Addgene_71505 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq validation vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23328393
Comments: We replaced the SV40 promotor of the pGL3-promotor plasmid by DSCP and inserted a Gateway-cassette into the multiple-cloning-site (MCS; using KpnI and BglII), i.e. upstream of the DSCP, to allow Gateway cloning of candidate sequences
Proper citation: RRID:Addgene_71506 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4.10; Vector Types:Mammalian Expression, humanSTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23328393
Comments: Note: The original human STARR-seq plasmid has been used in the Arnold et al. 2013 publication. It should be replaced for all screens in mammalian cells with the updated version reported by Muerdter et al. 2017 for much improved signal-to-noise, especially for screens with highly complex libraries (e.g. those covering an entire mammalian genome). The improved version is Addgene plasmid # 99296. For enhancer-activity measurements in mammalian cells, please also consider the possibility of a plasmid-induced innate immune response (see Muerdter et al., 2017).
The sequence between SacI and AfeI on pGL4 was replaced by the fragment containing the Super Core Promoter 1 (SCP1), a synthetic intron (pIRESpuro3, Clontech; cat. no. 631619) an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms, and the pGL3's SV40 late polyA-signal.
Proper citation: RRID:Addgene_71509 Copy
Species: Synthetic
Genetic Insert: mCherry-attR1/attR2
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Comments: Please note that mutation H30R in mCherry was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
Proper citation: RRID:Addgene_73487 Copy
Species: Synthetic
Genetic Insert: fli1ep
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73486 Copy
Species: Other
Genetic Insert: 6xmyc/attR1/attR2
Vector Backbone Description: Vector Backbone:pTol2005b; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73485 Copy
Species: Synthetic
Genetic Insert: fli1ep:EGFP-attR1-attR2
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73491 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5439; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27405979
Proper citation: RRID:Addgene_73637 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7215; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27405979
Proper citation: RRID:Addgene_73635 Copy
Species: Other
Genetic Insert: LSL-Tomato
Vector Backbone Description: Vector Backbone:Rosa26; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26018130
Proper citation: RRID:Addgene_66590 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5010; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26658467
Proper citation: RRID:Addgene_66937 Copy
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