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Vector Backbone Description: Backbone Marker:LM Guzman; Backbone Size:5779; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_47072 Copy
Vector Backbone Description: Backbone Marker:Eric R Geertsma & Bert Poolman; Backbone Size:5519; Vector Backbone:pRExLIC; Vector Types:Lactococcus lactis expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_47078 Copy
Vector Backbone Description: Backbone Marker:Eric R Geertsma & Bert Poolman; Backbone Size:6247; Vector Backbone:pRExLIC; Vector Types:Lactococcus lactis expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_47077 Copy
Vector Backbone Description: Backbone Marker:LM Guzman; Backbone Size:5824; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_47069 Copy
Vector Backbone Description: Backbone Marker:LM Guzman; Backbone Size:5779; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_47067 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5300; Vector Backbone:pMT/BiP/V5/His A; Vector Types:Insect Expression, Secreted expression in Drosophila Culture; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23827685
Comments: Inducible with 0.8 uM Copper Sulfate. Targets proteins to the extracellular millieu using the Drosophila melanogaster BiP signal peptide. Expresses in Drosophila melanogaster cell lines, such as Schneider 2 (S2) cells. Published in Ozkan et al, Cell 154(1): 228 (2013); PMID: 23827685; doi: 10.1016/j.cell.2013.06.006
After recombination, transform and grow in DH5alpha. The plasmid is no longer chloramphenicol resistant after recombination.
Proper citation: RRID:Addgene_47051 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4349; Vector Backbone:pMT/BiP/V5/His A; Vector Types:Insect Expression, Secreted expression in Drosophila Culture; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23827685
Comments: Inducible with 0.8 uM Copper Sulfate. Targets proteins to the extracellular millieu using the Drosophila melanogaster BiP signal peptide. Expresses in Drosophila melanogaster cell lines, such as Schneider 2 (S2) cells. Published in Ozkan et al, Cell 154(1): 228 (2013); PMID: 23827685; doi: 10.1016/j.cell.2013.06.006
After recombination, transform and grow in DH5alpha. The plasmid is no longer chloramphenicol resistant after recombination.
Proper citation: RRID:Addgene_47032 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR4; Vector Types:Mammalian Expression, PiggyBac; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23979020
Comments: For more information including protocols and
updates, please go to http://www.crispr-on.org
Proper citation: RRID:Addgene_48234 Copy
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate Compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed to take up inserts for future Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using HindIII and BamHI. The 3' BsaI overhang can be exchanged using KpnI and EcoRI. BamHI and KpnI sites as well as the double XcmI sites can also be used as alternative insert cloning sites, instead of using BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
BsaI site #2 = 3315-3325bp
Proper citation: RRID:Addgene_48863 Copy
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate Compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed to take up inserts for future Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using HindIII and BamHI. The 3' BsaI overhang can be exchanged using KpnI and EcoRI. BamHI and KpnI sites as well as the double XcmI sites can also be used as alternative insert cloning sites, instead of using BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
BsaI site #2 = 3315-3325bp
Proper citation: RRID:Addgene_48859 Copy
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate Compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed to take up inserts for future Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using HindIII and BamHI. The 3' BsaI overhang can be exchanged using KpnI and EcoRI. BamHI and KpnI sites as well as the double XcmI sites can also be used as alternative insert cloning sites, instead of using BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
BsaI site #2 = 3315-3325bp
Proper citation: RRID:Addgene_48858 Copy
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate Compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed to take up inserts for future Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using HindIII and BamHI. The 3' BsaI overhang can be exchanged using KpnI and EcoRI. BamHI and KpnI sites as well as the double XcmI sites can also be used as alternative insert cloning sites, instead of using BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
BsaI site #2 = 3315-3325bp
Proper citation: RRID:Addgene_48857 Copy
Vector Backbone Description: Backbone Marker:Open Biosystems; Backbone Size:11157; Vector Backbone:pLEXiPuro; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24146852
Proper citation: RRID:Addgene_48957 Copy
Vector Backbone Description: Backbone Marker:Open Biosystems; Backbone Size:11164; Vector Backbone:pLEXiPuro; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24146852
Proper citation: RRID:Addgene_48956 Copy
Vector Backbone Description: Backbone Marker:Rajewsky Lab, PMID 18327259 ; Vector Backbone:pSiP1; Vector Types:Luciferase, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24146852
Comments: The presence of firefly luciferase in the psiCHECK-2-derived vectors allows normalization of the Renilla luciferase expression that monitors the RNAi effect.
Proper citation: RRID:Addgene_48955 Copy
Vector Backbone Description: Backbone Marker:Imre Berger; Backbone Size:7720; Vector Backbone:pFBDM; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26043233
Comments: Constructed by Christopher Weinert, Grütter lab, Dept of Biochemistry, University of Zurich, Switzerland.
Proper citation: RRID:Addgene_49051 Copy
Vector Backbone Description: Backbone Marker:Lorenz et al, 1996 (PubMed ID: 8917596); Backbone Size:5246; Vector Backbone:pTLN; Vector Types:Xenopus oocytes expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Comments: Constructed by Dr. Iwan Zimmermann, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
Proper citation: RRID:Addgene_49032 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5855; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Comments: Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
Proper citation: RRID:Addgene_49030 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6578; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Comments: Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
Proper citation: RRID:Addgene_49029 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5862; Vector Backbone:pGL4; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25177895
Comments: Please note that the plasmid sequence contains an IS1 element, and the total size of the plasmid appears to be 7.5-8kb. This difference does not affect the function of the plasmid as described in the publication.
Proper citation: RRID:Addgene_49350 Copy
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