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Species: Drosophila melanogaster
Genetic Insert: Minimal Mos1, NeoR, peel-1, [4-3] Gateway
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4070; Vector Backbone:pDESTR4-R3; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24820376
Proper citation: RRID:Addgene_44477 Copy
Species: Drosophila melanogaster
Genetic Insert: Minimal Mos1, peel-1, [4-3] Gateway
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4070; Vector Backbone:pDESTR4-R3; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24820376
Proper citation: RRID:Addgene_44479 Copy
Species: C. Briggsae
Genetic Insert: Minimal Mos1, unc-119, [4-3] Gateway
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4070; Vector Backbone:pDESTR4-R3; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24820376
Proper citation: RRID:Addgene_44472 Copy
Vector Backbone Description: Backbone Marker:Connie Cepko lab (Harvard Medical School, Boston, MA); Backbone Size:7349; Vector Backbone:pEF-GFP (Addgene Plasmid #11154); Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18754012
Comments: Alternate plasmid name: pEF1α-RfC.1-IRES-GFP
To construct this vector, the depositing lab used EcoRI and BsrGI to replace the EGFP in pEF–GFP (Addgene plasmid 11154) with the IRES-GFP fragment from plasmid pBMN-I-GFP (Addgene plasmid 1736), creating intermediate vector pEF1α-IRES-GFP.
Plasmid pEF1α-IRES-GFP was then converted to a Gateway-enabled destination vector with the Gateway Vector Conversion System (Invitrogen) by digesting with EcoRI, blunting the ends, and inserting Reading Frame Cassette C (RfC.1) gateway cassette.
Proper citation: RRID:Addgene_45443 Copy
Vector Backbone Description: Backbone Size:9500; Vector Backbone:pCWX-DEST; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21590398
Comments: More information can be found at http://lentilab.unige.ch/ .
Proper citation: RRID:Addgene_45957 Copy
Vector Backbone Description: Backbone Size:10921; Vector Backbone:pCLX-pTF-DEST; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21761975
Comments: More information can be found at http://medweb2.unige.ch/salmon/lentilab/lentivectors.html
Proper citation: RRID:Addgene_45952 Copy
Vector Backbone Description: Backbone Marker:gift of W. Schaffner, University of Zurich; Backbone Size:6922; Vector Backbone:pSCT-GAL33VP80; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22966205
Proper citation: RRID:Addgene_46339 Copy
Genetic Insert: mir-1-1 reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression, RNAi, Expression of query pri-miRNA sequences in HEK293 cells; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23415231
Comments: Query pri-miRNA sequences are to be recombined into this plasmid using Gateway recombination. The human pri-mir-1-1 sequence is cloned downstream of the query pri-miRNA and is transcriptionally fused to the query pri-miRNA sequence. This plasmid was used for ectopic pri-miRNA expression in HEK293 cells
Proper citation: RRID:Addgene_46646 Copy
Genetic Insert: Gateway Cassette
Vector Backbone Description: Vector Backbone:pMartini; Vector Types:Gateway Conversion Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: This vector can be used for conversion of any cloning vector into a gateway destination vector and facilitate the generation of in-frame fusions with a gateway cassette.
A,B or C are reading frames; R1-R2 or R2-R1 indicate the gateway cassette orientation.
Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102
Proper citation: RRID:Addgene_36433 Copy
Genetic Insert: Gateway Cassette
Vector Backbone Description: Vector Backbone:pMartini; Vector Types:Gateway Conversion Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: This vector can be used for conversion of any cloning vector into a gateway destination vector and facilitate the generation of in-frame fusions with a gateway cassette.
A,B or C are reading frames; R1-R2 or R2-R1 indicate the gateway cassette orientation.
Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102
Proper citation: RRID:Addgene_36434 Copy
Genetic Insert: Gateway Cassette
Vector Backbone Description: Vector Backbone:pMartini; Vector Types:Gateway Conversion Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: This vector can be used for conversion of any cloning vector into a gateway destination vector and facilitate the generation of in-frame fusions with a gateway cassette.
A,B or C are reading frames; R1-R2 or R2-R1 indicate the gateway cassette orientation.
Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102
Proper citation: RRID:Addgene_36435 Copy
Genetic Insert: Gateway Cassette A R2-R1
Vector Backbone Description: Vector Backbone:pMartini; Vector Types:Gateway Conversion Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: This vector can be used for conversion of any cloning vector into a gateway destination vector and facilitate the generation of in-frame fusions with a gateway cassette.
A,B or C are reading frames; R1-R2 or R2-R1 indicate the gateway cassette orientation.
Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102
Proper citation: RRID:Addgene_36428 Copy
Vector Backbone Description: Backbone Marker:ATCC(pUC18) and Christensen and Quail(pAHC25); Backbone Size:4930; Vector Backbone:pUC18 and pAHC25; Vector Types:Plant expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_33297 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi14024 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290181
Proper citation: RRID:Addgene_34862 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi4391 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290181
Comments: There is a 461 bp deletion in the middle of the left recombination region. This deletion does not appear to adversely affect the ability to insert transgenes into the ttTi4391 site.
Proper citation: RRID:Addgene_34861 Copy
Species: Caenorhabditis elegans
Genetic Insert: cxTi10816 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290181
Comments: Please note there are TWO M13F primer sites. Use custom sequencing primers to sequence final gateway products.
Proper citation: RRID:Addgene_34864 Copy
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290182
Comments: New version of Neomycin resistance vector for drug selection in worms.
This vector replaces pBCN24-R4R3 (main advantage of the new vector is compatibility with ccdB Survival cells).
This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_34919 Copy
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290182
Comments: Dual resistance (PuroR-NeoR) vector for drug selection following biolistic bombardment in worms.
This vector contains a Pmyo-2::EGFP::myo-2_3'UTR pharyngeal marker on the backbone. Worm strains generated with vector can be crossed with strains generated with other visual markers such as in pBCN40-R4R3 which has a red fluorescent pharyngeal marker.
This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_34916 Copy
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:12020; Vector Backbone:pBID-UASC-MRG; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: Generation of ϕC31 transgenic Drosophila for GAL4 driven expression of myc tagRFP (tRFP) fusion transgenes. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter (DSCP). myc-tRFP is fused to the Nterminus of genes introduced by gateway. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinasemediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification.
Proper citation: RRID:Addgene_35205 Copy
Vector Backbone Description: Backbone Marker:LM Guzman; Backbone Size:5779; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_47074 Copy
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