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Species: Synthetic
Genetic Insert: hU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette
Vector Backbone Description: Backbone Size:7534; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30377374
Proper citation: RRID:Addgene_121944 Copy
Species: Synthetic
Genetic Insert: AaCas12b
Vector Backbone Description: Vector Backbone:BPK2014-ccdB; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30510770
Proper citation: RRID:Addgene_121949 Copy
Species: Synthetic
Genetic Insert: sgRNA-Sirius-4X(MS2-PP7)
Vector Backbone Description: Backbone Size:8171; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30377374
Proper citation: RRID:Addgene_121941 Copy
Species: Synthetic
Genetic Insert: sgRNA-Sirius-8XMS2
Vector Backbone Description: Backbone Size:8486; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30377374
Proper citation: RRID:Addgene_121942 Copy
Species: Synthetic
Genetic Insert: dCas9-P2A-HSA
Vector Backbone Description: Backbone Size:6645; Vector Backbone:pHAGE-TO; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30377374
Proper citation: RRID:Addgene_121936 Copy
Species: Synthetic
Genetic Insert: MCP-HALOnls
Vector Backbone Description: Backbone Size:6143; Vector Backbone:pHAGE-EFS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30377374
Proper citation: RRID:Addgene_121937 Copy
Species: Synthetic
Genetic Insert: PCP-GFPnls
Vector Backbone Description: Backbone Size:6143; Vector Backbone:pHAGE-EFS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30377374
Proper citation: RRID:Addgene_121938 Copy
Species: Synthetic
Genetic Insert: sgRNA-Sirius-8XMS2
Vector Backbone Description: Backbone Size:8171; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30377374
Proper citation: RRID:Addgene_121939 Copy
Species: Homo sapiens
Genetic Insert: GPCR activation based ACh sensor GRAB-ACh3.0
Vector Backbone Description: Backbone Size:4495; Vector Backbone:pAAV vector; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32989318
Proper citation: RRID:Addgene_121922 Copy
Species: Homo sapiens
Genetic Insert: GPCR activation based ACh sensor GRAB-ACh3.0
Vector Backbone Description: Backbone Size:4495; Vector Backbone:pAAV vector; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32989318
Proper citation: RRID:Addgene_121923 Copy
Species: Homo sapiens
Genetic Insert: p16-INK4A
Vector Backbone Description: Backbone Marker:David Root; Backbone Size:9354; Vector Backbone:pLX401 (pCW57.1, Addgene #41393); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30755442
Comments: p16-INK4A was codon-optimized for synthesis as a gBlock from Integrated DNA Technologies and Gateway cloned into pLX401 (pCW57.1, Addgene #41393).
Proper citation: RRID:Addgene_121919 Copy
Species: Mus musculus
Genetic Insert: IL-4 promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10970869
Comments: Murine IL-4 promotor from -804 to -3 relative to the transcription start site.
Proper citation: RRID:Addgene_12195 Copy
Species: Mus musculus
Genetic Insert: IL-2 promoter
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:0; Vector Backbone:pT81Luc; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10970869
Comments: Murine IL-2 promotor from -585 to -34 relative to the transcription start site.
Proper citation: RRID:Addgene_12194 Copy
Species: Homo sapiens
Genetic Insert: Mst1
Vector Backbone Description: Backbone Size:3500; Vector Backbone:pJ3M; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8702870
Proper citation: RRID:Addgene_12204 Copy
Species: Homo sapiens
Genetic Insert: Mst1
Vector Backbone Description: Backbone Size:3500; Vector Backbone:pJ3H; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8702870
Proper citation: RRID:Addgene_12203 Copy
Species: Homo sapiens
Genetic Insert: Mst2
Vector Backbone Description: Backbone Size:3500; Vector Backbone:pJ3H; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8702870
Comments: Addgene's QC finds additional E303D and T342M substitutions which are not known to affect function
Proper citation: RRID:Addgene_12206 Copy
Species: Homo sapiens
Genetic Insert: BRD4
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30500535
Comments: Vector contains SspB, a bacterial protein which binds exposed SsrA tag (from iLID) at high affinity.
Proper citation: RRID:Addgene_121968 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Choline kinase
Vector Backbone Description: Backbone Marker:Silva-Rocha,R., Martinez-Garcia,E., Chavarria,M., Calles,B., Arce-Rodriguez,A., de las Heras,A., Paez-Espino,D.; Backbone Size:3465; Vector Backbone:pSEVA228; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30584096
Proper citation: RRID:Addgene_122018 Copy
Species: Other
Genetic Insert: none
Vector Backbone Description: Backbone Size:6049; Vector Backbone:pUC57 and pWH1266; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31548010
Proper citation: RRID:Addgene_122000 Copy
Genetic Insert: none
Vector Backbone Description: Backbone Size:6073; Vector Backbone:pUC57 and pWH1266; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31548010
Proper citation: RRID:Addgene_121999 Copy
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