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Vector Backbone Description: Backbone Marker:PMID: 7720076; Backbone Size:4885; Vector Backbone:injection vector pRN3; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17878951
Comments: Gateway destination vector for mRNA production to overexpress a protein of interest following microinjection into ascidian eggs. Likely to be useful for zebrafish, Xenopus, urchin and cnidarian (Clytia, Nematostella...) overexpression studies as well. The injection vector pRN3 was modified to give rise to pSPE3 by inserting a new polylinker EcoR1-Stu1-Pme1-EcoRV-Not1 (GAATTCAGGCCTTTGTTTAAACTTAGATATCGCGGCCGC) between the EcoR1 and Not1 sites.
Proper citation: RRID:Addgene_186370 Copy
Species: Homo sapiens
Genetic Insert: DRD2
Vector Backbone Description: Backbone Size:5601; Vector Backbone:pcDNA3.1+/Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39148292
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.03.467169v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_190752 Copy
Species: Synthetic
Genetic Insert: sfGFP in E1.3a format
Vector Backbone Description: Vector Backbone:pSEVA331; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34767345
Comments: Read the preprint at https://www.biorxiv.org/content/10.1101/2021.06.09.447691v1.full
Proper citation: RRID:Addgene_180551 Copy
Species: Homo sapiens
Genetic Insert: NODAL variant mature peptide
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/early/2018/03/04/276170 for bioRxiv preprint.
Proper citation: RRID:Addgene_115265 Copy
Genetic Insert: T4 td upstream intron-PABP
Vector Backbone Description: Backbone Size:1882; Vector Backbone:pRC; Vector Types:Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35851375
Proper citation: RRID:Addgene_189972 Copy
Species: Homo sapiens
Genetic Insert: NODAL
Vector Backbone Description: Backbone Marker:Su-Chun Zhang, Addgene plasmid #52344; Vector Backbone:AAVS1-CAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/early/2018/03/04/276170 for bioRxiv preprint.
Proper citation: RRID:Addgene_115262 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Size:1882; Vector Backbone:pRC; Vector Types:Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35851375
Proper citation: RRID:Addgene_189976 Copy
Species: Homo sapiens
Genetic Insert: NODAL
Vector Backbone Description: Backbone Marker:Paul Krieg, Addgene plasmid # 17091; Vector Backbone:T7TS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/early/2018/03/04/276170 for bioRxiv preprint.
Proper citation: RRID:Addgene_115264 Copy
Species: Homo sapiens
Genetic Insert: AAVS1 homology arms with CMV-driven mRuby2-CAAX fusion
Vector Backbone Description: Backbone Marker:Thermo Fischer Scientific; Backbone Size:2974; Vector Backbone:pJET1.2; Vector Types:Mammalian Expression, CRISPR, Donor template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36416720
Proper citation: RRID:Addgene_183871 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:PGK-DTAG-CTERM; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36127368
Proper citation: RRID:Addgene_185770 Copy
Vector Backbone Description: Backbone Marker:PMID: 14736459; Backbone Size:4774; Vector Backbone:PDONR-221 P1-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:17878951
Comments: Backbone was modified with ApaI (attP3) / EcoRI (attP5) and PstI (attP3) / EcoRV (attP5) sites
Proper citation: RRID:Addgene_186350 Copy
Species: Homo sapiens
Genetic Insert: NODAL
Vector Backbone Description: Backbone Marker:Origene; Vector Backbone:pCMV6; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Internal MYC tag at N-terminal end of mature peptide. C312 is involved in interchain disuflide bond formation (by similarity). Please visit https://www.biorxiv.org/content/early/2018/03/04/276170 for bioRxiv preprint.
Proper citation: RRID:Addgene_115256 Copy
Species: Homo sapiens
Genetic Insert: NODAL
Vector Backbone Description: Backbone Marker:Origene; Vector Backbone:pCMV6; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Internal MYC tag at N-terminal end of mature peptide. NODAL mature peptide is truncated at the end of exon 2. Contains insert (cloning artifact) downstream of stop codon. Please visit https://www.biorxiv.org/content/early/2018/03/04/276170 for bioRxiv preprint.
Proper citation: RRID:Addgene_115257 Copy
Species: Homo sapiens
Genetic Insert: DPYSL2
Vector Backbone Description: Backbone Size:7438; Vector Backbone:pLJC2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35575798
Proper citation: RRID:Addgene_189866 Copy
Species: Homo sapiens
Genetic Insert: NODAL
Vector Backbone Description: Backbone Marker:Origene; Vector Backbone:pCMV6; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: C312 is involved in interchain disuflide bond formation (by similarity) . Please visit https://www.biorxiv.org/content/early/2018/03/04/276170 for bioRxiv preprint.
Proper citation: RRID:Addgene_115253 Copy
Species: Homo sapiens
Genetic Insert: signal peptide of human CD59, eGFP, aa 67-102 of human CD59 which contains the GPI attachment site
Vector Backbone Description: Backbone Marker:PMID: 14736459; Backbone Size:2544; Vector Backbone:PDONR-221 P1-P2; Vector Types:Expression of a fluorescent membrane marker; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17878951
Proper citation: RRID:Addgene_186355 Copy
Species: E. coli
Genetic Insert: Retron Eco1 Reverse Transcriptase
Vector Backbone Description: Vector Backbone:pJKR-O-mphR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35896746
Comments: A192P mutation in mphR codon optimized does not affect plasmid function.
Please visit https://doi.org/10.1101/2021.08.11.455990 for bioRxiv preprint.
Proper citation: RRID:Addgene_187202 Copy
Species: Homo sapiens
Genetic Insert: Pleckstrin Homology domain of PLCD1
Vector Backbone Description: Backbone Marker:PMID: 14736459; Backbone Size:2544; Vector Backbone:PDONR-221 P1-P2; Vector Types:Protein expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_186354 Copy
Vector Backbone Description: Backbone Marker:PMID: 14736459; Backbone Size:4768; Vector Backbone:PDONR-221 P1-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:17878951
Comments: Backbone was modified with ApaI (attP3) / EcoRI (attP4) and PstI (attP3) / EcoRV (attP4) sites
Proper citation: RRID:Addgene_186351 Copy
Species: Homo sapiens
Genetic Insert: BLK_E3-3_gRNA
Vector Backbone Description: Vector Backbone:pSPgRNA (Addgene #47108); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36160011
Proper citation: RRID:Addgene_124484 Copy
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