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Species: Homo sapiens
Genetic Insert: Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra)
Vector Backbone Description: Backbone Size:7609; Vector Backbone:pLL5.0; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31105010
Comments: Rescue with reconstituted FL Anillin.
5' cloning site: SacII (not destroyed), 3' cloning site: Sbf1 (not destroyed).
Proper citation: RRID:Addgene_187272 Copy
Species: Synthetic
Genetic Insert: synDNA 236
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:Synthetic Biology, Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36317886
Proper citation: RRID:Addgene_186182 Copy
Species: Homo sapiens
Genetic Insert: shRNA for Anillin
Vector Backbone Description: Backbone Size:7568; Vector Backbone:pLL5.0; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31105010
Comments: 5' cloning site: Hpa1 (destroyed), 3' cloning site: Xho1 (not destroyed).
Proper citation: RRID:Addgene_187270 Copy
Genetic Insert: Catalytically DEAD TET1
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36151095
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2021.09.11.459888v1.full
Proper citation: RRID:Addgene_184442 Copy
Genetic Insert: MS2-p65-hsf1
Vector Backbone Description: Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36151095
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2021.09.11.459888v1.full
Proper citation: RRID:Addgene_184440 Copy
Species: Homo sapiens
Genetic Insert: Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra)
Vector Backbone Description: Backbone Size:7590; Vector Backbone:pLL5.0; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31105010
Comments: Rescue with reconstituted -Anillin A740D,E758K.
5' cloning site: SacII (not destroyed), 3' cloning site: Sbf1 (destroyed).
With regard to all the Anillin sequences, I chose to number these residues as per the canonical sequence which is the full-length isoform. We have been using a smaller isoform, that lacks aa 508-544 as compared to the canonical isoform. The article that identified the Rho binding region on Anillin (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4449299/) followed the residue numbers of the canonical isoform to describe the mutations a740D, E758K (ref figure 2 in the article). Therefore, I chose to follow similar nomenclature when I generated the AHPH-DM for Rashmi's NCB paper. To harmonize the nomenclature across constructs, I continued to number the residues in all the GFP/Cherry Anillin constructs as per the canonical sequence.
Proper citation: RRID:Addgene_187276 Copy
Species: Synthetic
Genetic Insert: Soma-jGCaMP8f
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32592656
Proper citation: RRID:Addgene_182943 Copy
Species: Synthetic
Genetic Insert: Soma-GCaMP7
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32592656
Proper citation: RRID:Addgene_182942 Copy
Species: Synthetic
Genetic Insert: Soma-GCaMP7
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32592656
Proper citation: RRID:Addgene_182941 Copy
Species: Homo sapiens
Genetic Insert: MET
Vector Backbone Description: Vector Backbone:pRS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Cloning method: Site-directed mutagenesis.
Proper citation: RRID:Addgene_190459 Copy
Species: Other
Genetic Insert: ecTadA(8e)-neNme2-T.2
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36076084
Proper citation: RRID:Addgene_185668 Copy
Species: Other
Genetic Insert: ecTadA(8e)-neNme2-C
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36076084
Proper citation: RRID:Addgene_185667 Copy
Species: Mus musculus
Genetic Insert: ShRNA for Caveolin-1
Vector Backbone Description: Backbone Size:7917; Vector Backbone:pLL5.0; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33169848
Proper citation: RRID:Addgene_187257 Copy
Species: Synthetic
Genetic Insert: His8OphMATEV18mer
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.07.21.500988v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_189670 Copy
Species: Mus musculus
Genetic Insert: Reep5
Vector Backbone Description: Backbone Size:5345; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35471903
Proper citation: RRID:Addgene_186964 Copy
Species: Multiple
Genetic Insert: dCas9-KRAB(Kox1)-MeCP2
Vector Backbone Description: Vector Backbone:pMH0006, Addgene # 135448; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36576240
Comments: Human lentiviral vector for expression of dCas9 with a C-terminal HA-2xNLS-KRAB(Kox1)-MeCP2 fusion (no linker), and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element. pCL51. Please visit https://doi.org/10.1101/2022.07.13.499814 for bioRxiv preprint.
Proper citation: RRID:Addgene_188902 Copy
Species: Mus musculus
Genetic Insert: anti-NSD1 (Homo sapiens) recombinant Mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRCC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant antibody derived from RRID: AB_2921677. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_190311 Copy
Species: Vibrio cholerae
Genetic Insert: tnsA
Vector Backbone Description: Vector Backbone:R6K conditional origin, bla, RP4 oriT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:34873292
Comments: Clone guides using the 2xBsaI cloning site.
Clone Tn barcodes using the 2xBsmBI cloning site.
Proper citation: RRID:Addgene_186690 Copy
Species: Homo sapiens
Genetic Insert: Indoleamine 2,3-dioxygenase 1
Vector Backbone Description: Backbone Marker:Genscript; Vector Backbone:pcDNA3.1(+)-P2A-eGFP-C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35753585
Proper citation: RRID:Addgene_187026 Copy
Species: Synthetic
Genetic Insert: synDNA 156
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:Synthetic Biology, Unspecified, Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36317886
Proper citation: RRID:Addgene_186179 Copy
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