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Species: Other
Genetic Insert: Lifeact-mScarlet3-H
Vector Backbone Description: Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_242972 Copy
Species: Homo sapiens
Genetic Insert: pLV-U6-[SNCAintron1 gRNA 1]-EFSNC-dSaCas9-KRAB-MeCp2-P2A-Puro-WPRE
Vector Backbone Description: Backbone Marker:Duke VVC; Vector Backbone:Lentiviral vector; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30266652
Proper citation: RRID:Addgene_239797 Copy
Species: Synthetic
Genetic Insert: Citrine
Vector Backbone Description: Vector Backbone:pJT039; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39071298
Proper citation: RRID:Addgene_242613 Copy
Species: Homo sapiens
Genetic Insert: pLV-U6-[empty]-EFSNC-dSaCas9-DNMT3a-P2A-Puro-WPRE
Vector Backbone Description: Backbone Marker:Duke VVC; Vector Backbone:Lentiviral vector; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30266652
Proper citation: RRID:Addgene_239794 Copy
Species: Homo sapiens
Genetic Insert: pLV-U6-[empty]-EFSNC-dSaCas9-[empty linker]-P2A-Puro-WPRE
Vector Backbone Description: Backbone Marker:Duke VVC; Vector Backbone:Lentiviral vector; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30266652
Proper citation: RRID:Addgene_239793 Copy
Species: Homo sapiens
Genetic Insert: CMV/SNCAintron1-AlphaSyn[A53T]-WPRE-SV40pA
Vector Backbone Description: Backbone Marker:Duke VVC; Vector Backbone:AAV backbone; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30266652
Proper citation: RRID:Addgene_239792 Copy
Species: Homo sapiens
Genetic Insert: pLV-U6-[hSNCA gRNA 4]-EFSNC-dSpCas9-DNMT3a-P2A-PURO-WPRE
Vector Backbone Description: Backbone Marker:Duke VVC; Vector Backbone:Lentiviral vector; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30266652
Comments: Addgene NGS finds gRNA GGAGACGTGTACACGTCTCT.
Proper citation: RRID:Addgene_239839 Copy
Species: Homo sapiens
Genetic Insert: KEAP1
Vector Backbone Description: Vector Backbone:pBiT3.1-C [CMV/HiBiT/Blast] Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_238611 Copy
Species: Synthetic
Genetic Insert: AraC R2 S170 LOV P2.1.3
Vector Backbone Description: Vector Backbone:pDest; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41980963
Comments: Please visit https://doi.org/10.1101/2025.06.12.659331 for bioRxiv preprint.
Proper citation: RRID:Addgene_242155 Copy
Species: Mus musculus
Genetic Insert: Anti-c-Myc [9E10] light chain variable region fused to mouse kappa light chain constant region
Vector Backbone Description: Backbone Marker:Addgene Research Program (Addgene plasmid # 242954); Vector Backbone:pAG mKappa Empty; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_242956 Copy
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:4244; Vector Backbone:pAG AB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Plasmid 242954 pAG mKappa Empty encodes the mouse kappa light chain constant region and a filler sequence in lieu of a light chain variable region. To clone in your desired light chain variable region in frame with the mouse kappa light chain constant region, Addgene recommends the NEB Hifi assembly method.
PCR Amplify the backbone from plasmid 242954 pAG mKappa Empty with the following primers: 5’-cgggctgatgctgcaccaac and 5’-ggccctcgaggacgccagca.
Synthesize a gene fragment of the desired light chain variable region with flanking sequences that overlap with the backbone PCR product 5’-tgctggcgtcctcgagggcc-Gene fragment-cgggctgatgctgcaccaac
Assemble with the NEB HiFI assembly (NEB #E2621S) following the manufacturer’s recommendations
Proper citation: RRID:Addgene_242954 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40403729
Proper citation: RRID:Addgene_230120 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40403729
Proper citation: RRID:Addgene_230124 Copy
Species: Synthetic
Genetic Insert: Level1 OsU6-2p::sgRNA2 for subcloning of 20 bp target sequence
Vector Backbone Description: Backbone Marker:IDT; Vector Backbone:pUCIDT-AMP; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Further information can be found at https://www.growmorefoundation.org/enable-crispr-kits
Proper citation: RRID:Addgene_242204 Copy
Species: Synthetic
Genetic Insert: Level1 AtU6-26p::sgRNA1 for subcloning of 20 bp target sequence
Vector Backbone Description: Backbone Marker:VectorBuilder; Vector Backbone:pRP[Exp]; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Further information can be found at https://www.growmorefoundation.org/enable-crispr-kits
Proper citation: RRID:Addgene_242205 Copy
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: More information on https://www.growmorefoundation.org/enable-crispr-kits
Please note: This plasmid contains a C nucleotide deletion at P302 in dxs E.coli causing a frameshift and early termination. This deletion does not impact plasmid function.
Proper citation: RRID:Addgene_242207 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_243061 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_243062 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_243060 Copy
Species: Homo sapiens
Genetic Insert: PCSK5
Vector Backbone Description: Backbone Marker:Iain Fraser, Addgene plasmid #25752; Vector Backbone:pEN_TTmiRC2; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:41148168
Proper citation: RRID:Addgene_232449 Copy
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