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Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:35998606
Proper citation: RRID:Addgene_188972 Copy
Species: Other
Genetic Insert: Terminase
Vector Backbone Description: Backbone Size:3590; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35951700
Proper citation: RRID:Addgene_188855 Copy
Species: Homo sapiens
Genetic Insert: APOE
Vector Backbone Description: Backbone Size:8395; Vector Backbone:pTet-O; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: E4: rs429358-C (C112R) and rs7412-C
Proper citation: RRID:Addgene_190813 Copy
Species: Homo sapiens
Genetic Insert: RTN4B
Vector Backbone Description: Backbone Size:5345; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35471903
Proper citation: RRID:Addgene_186952 Copy
Species: Homo sapiens
Genetic Insert: APOE
Vector Backbone Description: Backbone Size:8395; Vector Backbone:pTet-O; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: E2: rs429358-T and rs7412-T (R158C)
Proper citation: RRID:Addgene_190811 Copy
Species: Mus musculus
Genetic Insert: Reverba-mScarlet
Vector Backbone Description: Backbone Marker:System Biosciences; Vector Backbone:PiggyBac Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35939672
Comments: Please note: Plasmid contains multiple nucleotide discrepancies and a 219bp discrepancy in the RevErb Insert compared to the depositor's provided sequence. These discrepancies are not known to affect plasmid function.
Proper citation: RRID:Addgene_190658 Copy
Species: Homo sapiens
Genetic Insert: APOE
Vector Backbone Description: Backbone Size:8395; Vector Backbone:pTet-O; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: E3: rs429358-T and rs7412-C
Proper citation: RRID:Addgene_190812 Copy
Species: Mus musculus
Genetic Insert: Ckap4
Vector Backbone Description: Backbone Size:5345; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35471903
Proper citation: RRID:Addgene_186957 Copy
Species: B/Brisbane/60/2008
Genetic Insert: polymerase acidic protein
Vector Backbone Description: Backbone Size:5426; Vector Backbone:pcDNA3; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35694292
Proper citation: RRID:Addgene_187922 Copy
Species: Homo sapiens
Genetic Insert: HUWE1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Entry clone; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34314700
Proper citation: RRID:Addgene_190464 Copy
Species: Synthetic
Genetic Insert: Marathon-RT
Vector Backbone Description: Vector Backbone:pCMV-T7, Addgene #132775; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36163548
Proper citation: RRID:Addgene_190108 Copy
Species: Synthetic
Genetic Insert: Marathon-RT
Vector Backbone Description: Vector Backbone:pCMV-T7, Addgene #132775; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36163548
Proper citation: RRID:Addgene_190110 Copy
Species: Homo sapiens
Genetic Insert: ncRNA targeting LRRK2-G2019S
Vector Backbone Description: Backbone Marker:Addgene Plasmid #132777; Backbone Size:3000; Vector Backbone:pU6-pegRNA-GG-acceptor; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36069759
Proper citation: RRID:Addgene_180435 Copy
Species: Homo sapiens
Genetic Insert: gRNA targeting LRRK2-G2019S
Vector Backbone Description: Backbone Marker:Jaenisch Laboratory, Soldner et al. Nature (2016) vol 533, pg 95-99; Backbone Size:10200; Vector Backbone:pX330-EGFP; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36069759
Proper citation: RRID:Addgene_180437 Copy
Species: Synthetic
Genetic Insert: promoter, RBS, mRFP1E_Orange
Vector Backbone Description: Backbone Marker:iGEM Registry; Vector Backbone:BioBrick pSB1C3 vector; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32891596
Proper citation: RRID:Addgene_160444 Copy
Species: Homo sapiens
Genetic Insert: ACE2
Vector Backbone Description: Backbone Size:5971; Vector Backbone:Promoterless Bxb1 recombination vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35895696
Proper citation: RRID:Addgene_190076 Copy
Species: Synthetic
Genetic Insert: synDNA 266
Vector Backbone Description: Backbone Marker:2710; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:Synthetic Biology, Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36317886
Proper citation: RRID:Addgene_186185 Copy
Species: Homo sapiens
Genetic Insert: Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra)
Vector Backbone Description: Backbone Size:7574; Vector Backbone:pLL5.0; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31105010
Comments: Rescue with reconstituted -Anillin A740D,E758K.
5' cloning site: SacII (destroyed), 3' cloning site: Sbf1 (not destroyed).
With regard to all the Anillin sequences, I chose to number these residues as per the canonical sequence which is the full-length isoform. We have been using a smaller isoform, that lacks aa 508-544 as compared to the canonical isoform. The article that identified the Rho binding region on Anillin (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4449299/) followed the residue numbers of the canonical isoform to describe the mutations a740D, E758K (ref figure 2 in the article). Therefore, I chose to follow similar nomenclature when I generated the AHPH-DM for Rashmi's NCB paper. To harmonize the nomenclature across constructs, I continued to number the residues in all the GFP/Cherry Anillin constructs as per the canonical sequence.
Proper citation: RRID:Addgene_187274 Copy
Species: Homo sapiens
Genetic Insert: Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra)
Vector Backbone Description: Backbone Size:7593; Vector Backbone:pLL5.0; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31105010
Comments: Rescue with reconstituted -Anillin A740D,E758K.
5' cloning site: SacII (not destroyed), 3' cloning site: Sbf1 (destroyed).
With regard to all the Anillin sequences, I chose to number these residues as per the canonical sequence which is the full-length isoform. We have been using a smaller isoform, that lacks aa 508-544 as compared to the canonical isoform. The article that identified the Rho binding region on Anillin (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4449299/) followed the residue numbers of the canonical isoform to describe the mutations a740D, E758K (ref figure 2 in the article). Therefore, I chose to follow similar nomenclature when I generated the AHPH-DM for Rashmi's NCB paper. To harmonize the nomenclature across constructs, I continued to number the residues in all the GFP/Cherry Anillin constructs as per the canonical sequence.
Proper citation: RRID:Addgene_187273 Copy
Species: Synthetic
Genetic Insert: synDNA 246
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:Synthetic Biology, Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36317886
Proper citation: RRID:Addgene_186183 Copy
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