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Species: Homo sapiens
Genetic Insert: interleukin-2 receptor alpha-subunit, Rhotekin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4688; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31105010
Comments: 5' cloning site: Nhe1 (not destroyed), 3' cloning site: Age1 (destroyed).
Proper citation: RRID:Addgene_187286 Copy
Species: Homo sapiens
Genetic Insert: LivePAR(R163A)
Vector Backbone Description: Backbone Marker:VectorBuilder; Vector Backbone:Lentivirus; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35954352
Proper citation: RRID:Addgene_187609 Copy
Genetic Insert: Opto-T7RNAP*(563)
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29045151
Proper citation: RRID:Addgene_101661 Copy
Genetic Insert: Opto-T7RNAP*(563), half expr.
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29045151
Proper citation: RRID:Addgene_101667 Copy
Genetic Insert: Opto-T7RNAP*(302)
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29045151
Proper citation: RRID:Addgene_101662 Copy
Genetic Insert: Opto-T7RNAP*(600)
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29045151
Proper citation: RRID:Addgene_101664 Copy
Species: Caenorhabditis elegans
Genetic Insert: Caenorhabditis elegans CNNM3 CBS-pair domain
Vector Backbone Description: Backbone Marker:Amersham-Pharmacia; Backbone Size:4984; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36822330
Proper citation: RRID:Addgene_166510 Copy
Species: Homo sapiens
Genetic Insert: miniTurbo-V5-CAMKK2
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_170577 Copy
Genetic Insert: na
Vector Backbone Description: Backbone Size:7743; Vector Backbone:pScalps hygro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33968806
Proper citation: RRID:Addgene_184785 Copy
Species: Homo sapiens
Genetic Insert: MKI67
Vector Backbone Description: Vector Backbone:pIRESpuro2; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32879492
Proper citation: RRID:Addgene_182921 Copy
Species: Synthetic
Genetic Insert: PPGK-driven expression of secreted Nluc
Vector Backbone Description: Backbone Size:2689; Vector Backbone:pVH1002 (pUC57-based); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33857582
Proper citation: RRID:Addgene_169616 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34912114
Proper citation: RRID:Addgene_186334 Copy
Species: Homo sapiens
Genetic Insert: FLT1
Vector Backbone Description: Vector Backbone:MAC-tag-C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35384245
Proper citation: RRID:Addgene_187784 Copy
Species: Homo sapiens
Genetic Insert: MKI67
Vector Backbone Description: Vector Backbone:pIRESpuro2; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27362226
Proper citation: RRID:Addgene_183748 Copy
Species: Homo sapiens
Genetic Insert: MKI67 (shuffled Nterm)
Vector Backbone Description: Backbone Size:5092; Vector Backbone:pIRESpuro2; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27362226
Proper citation: RRID:Addgene_183744 Copy
Species: Homo sapiens
Genetic Insert: LTK
Vector Backbone Description: Vector Backbone:MAC-tag-C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35384245
Proper citation: RRID:Addgene_187762 Copy
Species: Homo sapiens
Genetic Insert: Sec61
Vector Backbone Description: Backbone Size:5345; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35471903
Proper citation: RRID:Addgene_186959 Copy
Species: Caenorhabditis elegans
Genetic Insert: mTagBFP2
Vector Backbone Description: Backbone Size:6093; Vector Backbone:pSF11; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35715437
Proper citation: RRID:Addgene_184934 Copy
Species: Homo sapiens
Genetic Insert: DUX4-KBM
Vector Backbone Description: Vector Backbone:pDonor221; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35140242
Proper citation: RRID:Addgene_187802 Copy
Species: Mus musculus
Genetic Insert: Ckap4
Vector Backbone Description: Backbone Size:5345; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35471903
Proper citation: RRID:Addgene_186955 Copy
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