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Species: Saccharomyces cerevisiae
Genetic Insert: Ret2 I149A
Vector Backbone Description: Vector Backbone:p415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27298352
Proper citation: RRID:Addgene_73887 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Erp1 CT
Vector Backbone Description: Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27298352
Proper citation: RRID:Addgene_73920 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ret2 I155A
Vector Backbone Description: Vector Backbone:p415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27298352
Proper citation: RRID:Addgene_73888 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Inducible peptide of the dPSTR
Vector Backbone Description: Backbone Size:5053; Vector Backbone:pRS305; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27098003
Comments: This intermediate cloning vector is used to change the measured promoter (here pSTL11), by cloning it between SacI (or AatII) and XbaI (or SpeI). The entire inducible peptide has to be inserted into a pSIV by cloning AatII / SphI (into pDA183 or pDA200 for instance).
For more information, see Aymoz et al. Nat. Comm. 2016.
Proper citation: RRID:Addgene_74249 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pSTL1-dPSTRr(2,1)
Vector Backbone Description: Vector Backbone:pSIVU; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27098003
Comments: This vector contains the complete pSTL1-dPSTRr(2,1). The vector can be cut either PacI or BstBI prior to transformation in yeast. Selection in Ura depleted medium.
For more information, see Aymoz et al. Nat. Comm. 2016.
Proper citation: RRID:Addgene_74247 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: phosphoribosylaminoimidazole carboxylase ADE2
Vector Backbone Description: Backbone Size:3162; Vector Backbone:pUC118; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Constructed by T. J. Kappock in the laboratory of JoAnne Stubbe (MIT).
In JM109 cells.
Proper citation: RRID:Addgene_75060 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ret2LD2a N152T
Vector Backbone Description: Vector Backbone:p415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27298352
Proper citation: RRID:Addgene_75260 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ret2LD2a
Vector Backbone Description: Vector Backbone:p415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27298352
Proper citation: RRID:Addgene_75259 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Yeast-optimized dCas9
Vector Backbone Description: Vector Backbone:pRS304; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23977949
Proper citation: RRID:Addgene_64279 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TEF1 promoter
Vector Backbone Description: Backbone Marker:Mateus and Avery 2000; Vector Backbone:GFPCLN2PEST-pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25886317
Comments:
Proper citation: RRID:Addgene_64406 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUC2 promoter
Vector Backbone Description: Backbone Marker:Mateus and Avery 2000; Vector Backbone:GFPCLN2PEST-pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25886317
Comments:
Proper citation: RRID:Addgene_64405 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: g1 gRNA
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23977949
Proper citation: RRID:Addgene_64387 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast enhanced GFP
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23977949
Proper citation: RRID:Addgene_64389 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: c7 gRNA
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23977949
Proper citation: RRID:Addgene_64385 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast enhanced BFP2
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23977949
Proper citation: RRID:Addgene_64390 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast enhanced BFP2
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23977949
Proper citation: RRID:Addgene_64393 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ULP1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5368; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26297996
Proper citation: RRID:Addgene_64697 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AKAR4-NES
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21807900
Proper citation: RRID:Addgene_64727 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Orp1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pLPCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19755417
Proper citation: RRID:Addgene_64991 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AktAR2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25772363
Proper citation: RRID:Addgene_64932 Copy
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