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Species: Synthetic
Genetic Insert: IV-GTG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43777 Copy
Species: Synthetic
Genetic Insert: IV-GTT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43778 Copy
Species: Synthetic
Genetic Insert: IV-TAA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43779 Copy
Species: Synthetic
Genetic Insert: IV-GGA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43771 Copy
Species: Synthetic
Genetic Insert: IV-GGT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43774 Copy
Species: Synthetic
Genetic Insert: IV-GTA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43775 Copy
Species: Synthetic
Genetic Insert: IV-GAT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43766 Copy
Species: Synthetic
Genetic Insert: IV-GCA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43767 Copy
Species: Synthetic
Genetic Insert: IV-CTG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43761 Copy
Species: Synthetic
Genetic Insert: IV-CTT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43762 Copy
Species: Synthetic
Genetic Insert: IV-GAA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43763 Copy
Species: Synthetic
Genetic Insert: IV-GAC
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43764 Copy
Species: Synthetic
Genetic Insert: IV-CGA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43755 Copy
Species: Synthetic
Genetic Insert: IV-CGG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43757 Copy
Species: Synthetic
Genetic Insert: IV-CGT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43758 Copy
Species: Synthetic
Genetic Insert: IV-CAT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43750 Copy
Species: Synthetic
Genetic Insert: IV-CCG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43753 Copy
Species: Synthetic
Genetic Insert: IV-CCT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43754 Copy
Species: Synthetic
Genetic Insert: TT-ISceI-kan
Vector Backbone Description: Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:template; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:25255806
Comments: iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The kanamycin resistance gene was taken from pACYC177. We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script.
Proper citation: RRID:Addgene_59383 Copy
Vector Backbone Description: Backbone Size:4384; Vector Backbone:pBAM1; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21342504
Proper citation: RRID:Addgene_60487 Copy
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