Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: Danio rerio
Genetic Insert: Bin1a
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176013 Copy
Species: Danio rerio
Genetic Insert: Cavin4b (PRDmt)
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176017 Copy
Species: Danio rerio
Genetic Insert: Cavin4a
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176010 Copy
Species: Danio rerio
Genetic Insert: Cavin4b
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176008 Copy
Species: Danio rerio
Genetic Insert: Bin1a
Vector Backbone Description: Vector Backbone:SITS-EGFP-ccdb (Addgene 118628); Vector Types:Leishmania cell free expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176020 Copy
Species: Danio rerio
Genetic Insert: Bin1a
Vector Backbone Description: Vector Backbone:SITS-mCherry-ccdb (Addgene 118629); Vector Types:Leishmania cell free expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176024 Copy
Species: Danio rerio
Genetic Insert: zSynB
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3961; Vector Backbone:PCRII-TOPO; Vector Types:in-situ; Bacterial Resistance:Ampicillin
Comments: zSynB in situ probe.
For antisense, cut with XhoI and transcribe with SP6
Proper citation: RRID:Addgene_20122 Copy
Species: Danio rerio
Genetic Insert: mir-206-1/mir-133b
Vector Backbone Description: Backbone Marker:Bartel Lab; Backbone Size:0; Vector Backbone:pBSII SK+ modified to introduce GFP and more cloning sites; Vector Types:zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19240133
Comments: For polycistronic mir-206-1/mir-133b, we were not able to identify the transcriptional start precisely by 5′-RACE due to the repetitive sequence in this region. Analyses of EST clones (BM259633, CF999541, CF348604, DR729161) mapped the approximate start of the mir-206-1/mir-133b primary transcript to 1.4 kb from the mir-206 hairpin. A 4.5-kb upstream fragment that includes ∼0.9 kb of the primary transcript was amplified from genomic DNA using primers 3 and 4 (5'-CTGCCCCATTTAGACTCTTTCTTGACG-3' and 5'-CCAGAAGAACCCAGACGAAACCCAC-3'). The promoter fragment was fused to a GFP reporter gene. The miRNA promoter fusion was flanked by I-SceI meganuclease recognition sites to increase the efficiency of transgenensis.
Please note that Addgene's sequencing results show some differences from the published sequence. The depositing lab is aware of the differences.
Proper citation: RRID:Addgene_20793 Copy
Species: Danio rerio
Genetic Insert: mir-124
Vector Backbone Description: Backbone Marker:Bartel Lab; Backbone Size:0; Vector Backbone:pBSII SK+ modified to introduce GFP and more cloning sites; Vector Types:zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19240133
Comments: 5′-RACE (GenRacer kit, Invitrogen) identified a transcriptional start of the mir-124-5 primary transcript, which is located 1.1 kb from the mir-124-5 hairpin. A 5.6-kb fragment upstream of the mir-124-5 transcriptional start was amplified from genomic DNA using primers 1 and 2 (5'-TGCTTTGAGGCACGGTTATGGTCCC-3' and 5'-CTCGGGCAGCGGCTTTTTGGTCCTG-3'). The promoter fragment was fused to a GFP reporter gene. The miRNA promoter fusion was flanked by I-SceI meganuclease recognition sites to increase the efficiency of transgenensis.
Proper citation: RRID:Addgene_20792 Copy
Species: Danio rerio
Genetic Insert: F1 GAC
Vector Backbone Description: Backbone Size:6141; Vector Backbone:pCS2 (modified); Vector Types:mRNA production; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21937602
Proper citation: RRID:Addgene_30694 Copy
Species: Danio rerio
Genetic Insert: F1 GCG
Vector Backbone Description: Backbone Size:4789; Vector Backbone:pCS2; Vector Types:mRNA production; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21937602
Proper citation: RRID:Addgene_30699 Copy
Species: Danio rerio
Genetic Insert: F1 GCC
Vector Backbone Description: Backbone Size:6141; Vector Backbone:pCS2; Vector Types:mRNA production; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21937602
Proper citation: RRID:Addgene_30698 Copy
Species: Danio rerio
Genetic Insert: F2 AGA
Vector Backbone Description: Backbone Size:2877; Vector Backbone:pBlueScript II SK(+); Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21937602
Proper citation: RRID:Addgene_30756 Copy
Species: Danio rerio
Genetic Insert: F3 AGA
Vector Backbone Description: Backbone Size:2877; Vector Backbone:pBlueScript II SK(+); Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21937602
Proper citation: RRID:Addgene_30757 Copy
Species: Danio rerio
Genetic Insert: F3 GCC
Vector Backbone Description: Backbone Size:2877; Vector Backbone:pBlueScript II SK(+); Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21937602
Proper citation: RRID:Addgene_30740 Copy
Species: Danio rerio
Genetic Insert: F2 GCT
Vector Backbone Description: Backbone Size:6141; Vector Backbone:pCS2; Vector Types:mRNA production; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21937602
Proper citation: RRID:Addgene_30723 Copy
Species: Danio rerio
Genetic Insert: pCS4+-P2A
Vector Backbone Description: Backbone Size:4146; Vector Backbone:pCS4; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21602908
Proper citation: RRID:Addgene_31566 Copy
Species: Danio rerio
Genetic Insert: TAL array targeting gria3a
Vector Backbone Description: Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: TAL N-terminal: ∆152 (Miller et al. 2011)
TAL C-terminus: +63 (Miller et al. 2011)
FOKI: WT
Proper citation: RRID:Addgene_32283 Copy
Species: Danio rerio
Genetic Insert: TAL array targeting gria3a
Vector Backbone Description: Vector Backbone:JDS78; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: TAL N-terminal: ∆152 (Miller et al. 2011)
TAL C-terminus: +63 (Miller et al. 2011)
FOKI: WT
Proper citation: RRID:Addgene_32281 Copy
Species: Danio rerio
Genetic Insert: TAL array targeting gria3a
Vector Backbone Description: Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: TAL N-terminal: ∆152 (Miller et al. 2011)
TAL C-terminus: +63 (Miller et al. 2011)
FOKI: WT
Proper citation: RRID:Addgene_32282 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the dkNET Resources search. From here you can search through a compilation of resources used by dkNET and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that dkNET has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on dkNET then you can log in from here to get additional features in dkNET such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into dkNET you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within dkNET that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.