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Vector Backbone Description: Backbone Size:4360; Vector Backbone:pBR322; Vector Types:Synthetic Biology, ; Episomal vector for Phaeodactylum tricornutum; Bacterial Resistance:Ampicillin and Tetracycline
Proper citation: RRID:Addgene_80388 Copy
Species: Synthetic
Genetic Insert: T7A1, O-, O1, Lambda t1 in lacZ fragment
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
Comments: LacI operators separated by 400 base pairs.
Proper citation: RRID:Addgene_170473 Copy
Species: Synthetic
Genetic Insert: T7A1, Os, O2, Lambda t1 in lacZ fragment
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
Comments: LacI operators separated by 400 base pairs.
Proper citation: RRID:Addgene_170471 Copy
Species: Synthetic
Genetic Insert: T7A1, O2, O1, Lambda t1 in lacZ fragment
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:28295806
Comments: LacI operators separated by 400 base pairs.
Proper citation: RRID:Addgene_170477 Copy
Species: Synthetic
Genetic Insert: T7A1, O2, Os, Lambda t1 in lacZ fragment
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
Comments: LacI operators separated by 400 base pairs.
Proper citation: RRID:Addgene_170478 Copy
Species: Mus musculus
Genetic Insert: CHOP10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:1547942
Comments: Addgene sequencing results show that only half of the leurin zipper is deleted, protein ends at amino acid 133.
Note that the phagemid had a scrambled insert and the portion 3' of the CHOP-10 coding region (in red-see author's map) is not from the CHOP gene. If you wish to obtain a CHOP probe for hybridization experiments we suggest excising the HD3-Nhe1 fragment that contains CHOP coding sequence.
Note that this plasmid must be propagated in P3-containing host strain such as DH10B/P3 under combined amp (25mcg/ml)/tet (10mcg/ml) selection pressure.
Proper citation: RRID:Addgene_21900 Copy
Genetic Insert: tetA(C)
Vector Backbone Description: Backbone Marker:Alexeyev, M.F., BioTechniques 18:52-56, 1995; Vector Backbone:pBSL15; Vector Types:Bacterial vector based on pMB1 replicon; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:26921431
Proper citation: RRID:Addgene_74110 Copy
Species: Synthetic
Genetic Insert: tetA(C)
Vector Backbone Description: Vector Backbone:pUCBM21; Vector Types:Conjugative bacterial vector; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:26921431
Proper citation: RRID:Addgene_74104 Copy
Genetic Insert: tetA
Vector Backbone Description: Backbone Size:8754; Vector Backbone:pMJH46; Vector Types:Bacterial Expression, Red Recombinase, temperature sensitive; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:28352570
Comments: Conjugally transferable red recombinase vector pMJH65
Proper citation: RRID:Addgene_67273 Copy
Genetic Insert: Flp recombinase
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pFLP1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:15908923
Comments: GenBank Accession # AY597273
Compared to the Genbank reference sequence AY597273, Addgene's sequencing results found a T350I mutation in the tetracycline cassette and a G5D mutation in FLP recombinase. The effect of these differences is unknown.
Proper citation: RRID:Addgene_64946 Copy
Genetic Insert: FRT-TcR-FRT
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pFRT2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:15908923
Comments: GenBank Accession #AY712950
Compared to the Genbank reference sequence AY712950, Addgene's sequencing results found a T350I mutation in the tetracycline cassette. The effect of this difference is unknown.
Proper citation: RRID:Addgene_64951 Copy
Species: Homo sapiens
Genetic Insert: RAP1
Vector Backbone Description: Backbone Marker:Anumanthan et al. 1998; Vector Backbone:pAXEF-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:15469846
Proper citation: RRID:Addgene_32697 Copy
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