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Species: Vibrio cholerae
Genetic Insert: tnsA
Vector Backbone Description: Vector Backbone:R6K conditional origin, bla, RP4 oriT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34873292
Comments: Clone guides using the 2xBsaI cloning site.
Clone Tn barcodes using the 2xBsmBI cloning site.
Clone any VcDART cargo of interest using the 2xLguI cargo cloning site inside the Tn7 transposon.
Proper citation: RRID:Addgene_186695 Copy
Species: Vibrio cholerae
Genetic Insert: tnsA
Vector Backbone Description: Vector Backbone:R6K conditional origin, bla, RP4 oriT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:34873292
Comments: Clone guides using the 2xBsaI cloning site.
Clone Tn barcodes using the 2xBsmBI cloning site.
This vector is identical to pBFC0619, but it includes a dual restriction site (AsiSI+SbfI) flanking the Tn right end to reduce donor vector during ET-Seq.
Proper citation: RRID:Addgene_186694 Copy
Species: Scytonema hofmanni
Genetic Insert: tnsB
Vector Backbone Description: Vector Backbone:R6K conditional origin, bla, RP4 oriT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:34873292
Comments: Clone Tn barcodes using the 2xBsmBI cloning site.
Proper citation: RRID:Addgene_186693 Copy
Species: Mus musculus
Genetic Insert: Hsp25
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pLenti6.3/V5-DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_187703 Copy
Species: Mus musculus
Genetic Insert: Hsp25
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pLenti6.3/V5-DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_187702 Copy
Genetic Insert: CVB3 IRES
Vector Backbone Description: Backbone Size:1882; Vector Backbone:pRC; Vector Types:Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35851375
Proper citation: RRID:Addgene_189968 Copy
Species: Synthetic
Genetic Insert: wgaAB bicistronic
Vector Backbone Description: Vector Backbone:pSEVA531; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:33465305
Proper citation: RRID:Addgene_186262 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:pSEVA531; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:33465305
Proper citation: RRID:Addgene_186261 Copy
Species: Acytostelium subglobosum
Genetic Insert: Acytostelium subglobosum PRL
Vector Backbone Description: Backbone Marker:Novagen Inc., Madison, WI; Backbone Size:5708; Vector Backbone:pET-15B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36822330
Proper citation: RRID:Addgene_166415 Copy
Species: Mus musculus
Genetic Insert: anti-Nav1.8 Na+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRCC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant antibody derived from RRID: AB_2916236. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_188203 Copy
Species: Galdieria sulphuraria
Genetic Insert: Galdieria sulphuraria PRL
Vector Backbone Description: Backbone Marker:Novagen Inc., Madison, WI; Backbone Size:5708; Vector Backbone:pET-15B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36822330
Proper citation: RRID:Addgene_166419 Copy
Species: Drosophila melanogaster
Genetic Insert: Drosophila melanogaster PRL
Vector Backbone Description: Backbone Marker:Novagen Inc., Madison, WI; Backbone Size:5708; Vector Backbone:pET-15B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36822330
Proper citation: RRID:Addgene_166418 Copy
Species: Homo sapiens
Genetic Insert: ROS1
Vector Backbone Description: Vector Backbone:MAC-tag-C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35384245
Proper citation: RRID:Addgene_187766 Copy
Species: Homo sapiens
Genetic Insert: PolB
Vector Backbone Description: Backbone Marker:Sigma; Vector Backbone:pGEX4T3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31287140
Proper citation: RRID:Addgene_177133 Copy
Species: Synthetic
Genetic Insert: EcoRNAPα-PmCDA1
Vector Backbone Description: Backbone Size:5023; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34928386
Proper citation: RRID:Addgene_189824 Copy
Species: Homo sapiens
Genetic Insert: interleukin-2 receptor alpha-subunit, anillin, RhoA
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4694; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31105010
Comments: 5' cloning site: Nhe1 (destroyed), 3' cloning site: Xba1 (not destroyed).
Proper citation: RRID:Addgene_187292 Copy
Species: Naegleria fowleri
Genetic Insert: Naegleria fowleri CBS-pair domain
Vector Backbone Description: Backbone Marker:Amersham-Pharmacia; Backbone Size:4984; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36822330
Proper citation: RRID:Addgene_166437 Copy
Species: Caenorhabditis elegans
Genetic Insert: Caenorhabditis elegans CNNM5 CBS-pair domain
Vector Backbone Description: Backbone Marker:Amersham-Pharmacia; Backbone Size:4984; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36822330
Proper citation: RRID:Addgene_166434 Copy
Species: Other
Genetic Insert: eNme2-C.NR nuclease
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36076084
Proper citation: RRID:Addgene_185672 Copy
Species: Other
Genetic Insert: ecTadA(8e)-nSpRY-HF1
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36076084
Proper citation: RRID:Addgene_185670 Copy
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