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Species: Synthetic
Genetic Insert: 5'UTR-PE7-3'UTR-polyA
Vector Backbone Description: Backbone Marker:Addgene #207455; Vector Backbone:IVT-nCas9(H840A); Vector Types:Mammalian Expression, CRISPR, in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41813887
Proper citation: RRID:Addgene_248222 Copy
Species: Synthetic
Genetic Insert: Bxb1 attB
Vector Backbone Description: Backbone Marker:Addgene #139987; Vector Backbone:pCMV-T7-SpCas9-P2A-EGFP (RTW3027); Vector Types:Mammalian Expression, CRISPR, in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41813887
Proper citation: RRID:Addgene_248223 Copy
Species: Homo sapiens
Genetic Insert: PGE2 sensor GRAB_PGE2-1.0
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:5119; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41831442
Comments: Please visit https://doi.org/10.1101/2025.06.01.657218 for the bioRxiv preprint.
Proper citation: RRID:Addgene_251355 Copy
Species: Synthetic
Genetic Insert: 5'UTR-SuperDn29-bpNLS-3'UTR-polyA
Vector Backbone Description: Backbone Marker:Addgene #207455; Vector Backbone:IVT-nCas9(H840A); Vector Types:Mammalian Expression, CRISPR, in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41813887
Proper citation: RRID:Addgene_248220 Copy
Species: Synthetic
Genetic Insert: SacINT attP
Vector Backbone Description: Backbone Marker:Addgene #139987; Vector Backbone:pCMV-T7-SpCas9-P2A-EGFP (RTW3027); Vector Types:Mammalian Expression, CRISPR, in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41813887
Proper citation: RRID:Addgene_248227 Copy
Species: SARS-CoV-2
Genetic Insert: CoV-2_frD2_ΔORF67a8
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37988285
Comments: Detailed explanation of CLEVER reverse genetics methodology in https://doi.org/10.7554/eLife.89035
Proper citation: RRID:Addgene_253130 Copy
Species: SARS-CoV-2
Genetic Insert: CoV-2_frD_ORF6-BirA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37988285
Comments: Detailed explanation of CLEVER reverse genetics methodology in https://doi.org/10.7554/eLife.89035
Proper citation: RRID:Addgene_253132 Copy
Species: Homo sapiens
Genetic Insert: UBAP2L Nterm Guide RNA 1
Vector Backbone Description: Backbone Size:8484; Vector Backbone:pX333; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_246894 Copy
Species: Homo sapiens
Genetic Insert: Caprin1 Nterm Guide RNA 1
Vector Backbone Description: Backbone Size:8484; Vector Backbone:pX332; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_246893 Copy
Species: Homo sapiens
Genetic Insert: G3BP2 Nterm Guide RNA 1
Vector Backbone Description: Backbone Size:8484; Vector Backbone:pX331; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_246892 Copy
Species: Homo sapiens
Genetic Insert: G3BP1 Nterm Guide RNA 1
Vector Backbone Description: Backbone Size:8484; Vector Backbone:pX330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_246891 Copy
Species: Homo sapiens
Genetic Insert: RB1::ecDHFR::NanoLuc::MS2v5 + Blasticidin resistance piggybac
Vector Backbone Description: Backbone Size:6613; Vector Backbone:pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_246897 Copy
Species: Homo sapiens
Genetic Insert: eGFP::CRY2::G3BP1 (no dimerization domain) + Hygro resistance
Vector Backbone Description: Backbone Size:6613; Vector Backbone:pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_246896 Copy
Species: Homo sapiens
Genetic Insert: cofilin 1 (cfl1)
Vector Backbone Description: Backbone Marker:Gift from Bert Vogelstein in 1999; Backbone Size:7470; Vector Backbone:pShuttle CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22573689
Comments: Series of next 7 plasmids are single or double replacements of cysteine residues in pShuttle
Proper citation: RRID:Addgene_245966 Copy
Species: Synthetic
Genetic Insert: LaTranC mini-sgRNA
Vector Backbone Description: Vector Backbone:phU6; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41027434
Proper citation: RRID:Addgene_246934 Copy
Species: Homo sapiens
Genetic Insert: DDIT4L::ecDHFR::NanoLuc::MS2v5 + Blasticidin resistance piggybac
Vector Backbone Description: Backbone Size:6613; Vector Backbone:pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_246899 Copy
Species: Homo sapiens
Genetic Insert: cofilin 1 (cfl1)
Vector Backbone Description: Backbone Marker:Gift from Bert Vogelstein in 1999; Backbone Size:7470; Vector Backbone:pShuttle CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22573689
Proper citation: RRID:Addgene_245969 Copy
Species: Homo sapiens
Genetic Insert: cofilin 1 (cfl1)
Vector Backbone Description: Backbone Marker:Gift from Bert Vogelstein in 1999; Backbone Size:7470; Vector Backbone:pShuttle CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22573689
Proper citation: RRID:Addgene_245970 Copy
Species: Homo sapiens
Genetic Insert: cofilin 1 (cfl1)
Vector Backbone Description: Backbone Size:7981; Vector Backbone:pShuttle NSE; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19513994
Comments: Neuronal specific expression of mRFP-tagged WT cofilin
Proper citation: RRID:Addgene_245973 Copy
Species: Homo sapiens
Genetic Insert: cofilin 1 (cfl1)
Vector Backbone Description: Backbone Marker:Gift from Bert Vogelstein in 1999; Backbone Size:7470; Vector Backbone:pShuttle CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22573689
Comments: Two cysteines modified here form a disulfide bond in cofilactin bundles isolated from stressed neurons.
Proper citation: RRID:Addgene_245972 Copy
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