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Species: SARS-CoV-2, Influenza virus PR8
Genetic Insert: CoV-2 frD2 with Influenza H1 PR8
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37988285
Comments: detailed explanation of CLEVER reverse genetics methodology in DOI: 10.7554/eLife.89035
Proper citation: RRID:Addgene_253147 Copy
Species: SARS-CoV-2, Influenza virus PR8
Genetic Insert: CoV-2 frD2 with Influenza N1 PR8
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37988285
Comments: detailed explanation of CLEVER reverse genetics methodology in DOI: 10.7554/eLife.89035
Proper citation: RRID:Addgene_253150 Copy
Species: Arabidopsis thaliana
Genetic Insert: BSL2/BSL1 chimera
Vector Backbone Description: Vector Backbone:pCambia-3300; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42412928
Proper citation: RRID:Addgene_256023 Copy
Species: Arabidopsis thaliana
Genetic Insert: TOPP1
Vector Backbone Description: Vector Backbone:pCambia-3300; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42412928
Proper citation: RRID:Addgene_256029 Copy
Vector Backbone Description: Backbone Size:17180; Vector Backbone:pGEF-U; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_252743 Copy
Genetic Insert: pegRNA cloning vector
Vector Backbone Description: Vector Backbone:puc19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42127219
Proper citation: RRID:Addgene_251104 Copy
Species: Synthetic
Genetic Insert: CsL4.2-15
Vector Backbone Description: Backbone Size:2762; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42259793
Proper citation: RRID:Addgene_242350 Copy
Species: Synthetic
Genetic Insert: CsL4.2-20
Vector Backbone Description: Backbone Size:2762; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42259793
Proper citation: RRID:Addgene_242351 Copy
Species: Synthetic
Genetic Insert: CsL4.2-20
Vector Backbone Description: Backbone Size:6008; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42259793
Proper citation: RRID:Addgene_242355 Copy
Species: Synthetic
Genetic Insert: L13-9
Vector Backbone Description: Backbone Size:6008; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42259793
Proper citation: RRID:Addgene_242352 Copy
Species: Synthetic
Genetic Insert: L15-20
Vector Backbone Description: Backbone Size:6008; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42259793
Proper citation: RRID:Addgene_242353 Copy
Species: Synthetic
Genetic Insert: 5'UTR-bpNLS-BxbI-3'UTR-polyA
Vector Backbone Description: Backbone Marker:Addgene #207455; Vector Backbone:IVT-nCas9(H840A); Vector Types:Mammalian Expression, CRISPR, in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41813887
Proper citation: RRID:Addgene_248216 Copy
Species: Synthetic
Genetic Insert: superfolder GFP
Vector Backbone Description: Backbone Size:5058; Vector Backbone:pcDNA4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42259793
Proper citation: RRID:Addgene_242356 Copy
Species: Homo sapiens
Genetic Insert: tet3G-cmv-FRB-SMG7C-IRES-FKBP-HaloTag-tdMCP-NLS-HA
Vector Backbone Description: Backbone Size:8545; Vector Backbone:PSR1903 tet3G; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38548718
Proper citation: RRID:Addgene_232461 Copy
Species: Homo sapiens
Genetic Insert: PERIOD 2 Promoter
Vector Backbone Description: Backbone Marker:Alexeyev Lab; Backbone Size:8331; Vector Backbone:pMA3160; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41226820
Comments: For generation of PER2-luciferase (PER2-Luc) plasmid, a 952-bp EcoRI/NotI fragment was isolated from a pGL3 basic PER2 construct, obtained from Dr. Helmut Zarbl (Rutgers University). The PER2 promoter-containing fragment was subcloned into the lentiviral construct pMA3160 (Addgene plasmid #35043, deposited by Dr Mikhail Alexeyev) to generate a PER2-luciferase reporter construct.
Proper citation: RRID:Addgene_250937 Copy
Species: SARS-CoV-2
Genetic Insert: Fragment D2 SARS-CoV-2 ΔE-GFPΔ6Δ8
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37988285
Comments: Detailed explanation of CLEVER reverse genetics methodology in https://doi.org/10.7554/eLife.89035
Proper citation: RRID:Addgene_253121 Copy
Species: SARS-CoV-2
Genetic Insert: Fragment D2 SARS-CoV-2 ΔEΔ6Δ7 orf3*
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37988285
Comments: Detailed explanation of CLEVER reverse genetics methodology in https://doi.org/10.7554/eLife.89035
Proper citation: RRID:Addgene_253122 Copy
Species: SARS-CoV-2
Genetic Insert: CoV-2_frD2_ΔORF7a
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37988285
Comments: Detailed explanation of CLEVER reverse genetics methodology in https://doi.org/10.7554/eLife.89035
Proper citation: RRID:Addgene_253126 Copy
Genetic Insert: PE7 C-terminal
Vector Backbone Description: Vector Backbone:puc19; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42127219
Proper citation: RRID:Addgene_251106 Copy
Species: SARS-CoV-2
Genetic Insert: CoV-2_frD2_ΔORF7b
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37988285
Comments: Detailed explanation of CLEVER reverse genetics methodology in https://doi.org/10.7554/eLife.89035
Proper citation: RRID:Addgene_253127 Copy
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