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Species: Synthetic
Genetic Insert: L13-23
Vector Backbone Description: Backbone Size:2762; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42259793
Proper citation: RRID:Addgene_242348 Copy
Species: Synthetic
Genetic Insert: wild-type class B tetracycline repressor
Vector Backbone Description: Backbone Size:2762; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42259793
Proper citation: RRID:Addgene_242345 Copy
Species: Synthetic
Genetic Insert: L12-11
Vector Backbone Description: Backbone Size:2762; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42259793
Proper citation: RRID:Addgene_242346 Copy
Genetic Insert: prime editing guideRNA
Vector Backbone Description: Vector Backbone:sgOpti; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40245860
Proper citation: RRID:Addgene_255532 Copy
Species: E. coli
Genetic Insert: dnaX
Vector Backbone Description: Vector Backbone:pET-3c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The Ec dnaX gene native sequence, which is in this plasmid, has a frameshift that happens with approximately 50% efficiency, resulting in approximately equal expression of the gamma and tau subunits of E. coli DNA Polymerase III from this clone.
Proper citation: RRID:Addgene_255907 Copy
Species: SARS-CoV-2, Influenza virus PR8
Genetic Insert: CoV-2 frD with Influenza N1 PR8
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37988285
Comments: detailed explanation of CLEVER reverse genetics methodology in DOI: 10.7554/eLife.89035
Proper citation: RRID:Addgene_253169 Copy
Species: Homo sapiens
Genetic Insert: ANKRD11
Vector Backbone Description: Vector Backbone:pCAG-puro-3xFLAG (DEST); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40520101
Proper citation: RRID:Addgene_236621 Copy
Species: Salmonella typhimurium
Genetic Insert: 2-deoxyribose-5-phosphate aldolase (DERA) from Salmonella typhimurium
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5708; Vector Backbone:pET 15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31806708
Proper citation: RRID:Addgene_255517 Copy
Species: Moloney murine leukemia virus
Genetic Insert: MMLV-RT
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:4723; Vector Backbone:pQE-81L; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_252404 Copy
Species: Thermotoga maritima
Genetic Insert: 2-deoxyribose-5-phosphate aldolase (DERA) from Thermotoga maritima
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5708; Vector Backbone:pET 15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31806708
Proper citation: RRID:Addgene_255518 Copy
Species: SARS coronavirus
Genetic Insert: 3CL protease
Vector Backbone Description: Backbone Marker:Novagen/NESG; Backbone Size:5681; Vector Backbone:pET15SUMO2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Related to PDB: 4YLU
Proper citation: RRID:Addgene_240380 Copy
Species: Homo sapiens
Genetic Insert: hnRNPK
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32813011
Comments: Mouse and human hnRNPK are 100% identical.
Primers were used to PCR amplify cDNA insert for ligation into a pET28b vector containing an N-terminal 10xHis-SUMO tag using the Quick Ligation kit (NEB).
Proper citation: RRID:Addgene_252528 Copy
Species: Homo sapiens
Genetic Insert: mCherry-TFEB fusion
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8932; Vector Backbone:pLenti4/TO/V5-DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_252409 Copy
Species: Middle East Respiratory Syndrome-related coronavirus
Genetic Insert: 3CL protease
Vector Backbone Description: Backbone Marker:Novagen backbone modified in the lab of Gaetano Montelione; Backbone Size:5681; Vector Backbone:pET15SUMO2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Related to PDB: 4YLU
Proper citation: RRID:Addgene_240381 Copy
Species: Human poliovirus 1 Mahoney
Genetic Insert: 3C protease
Vector Backbone Description: Backbone Marker:Novagen/NESG; Backbone Size:5681; Vector Backbone:pET15SUMO2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Related to PDB: 1L1N
Proper citation: RRID:Addgene_240387 Copy
Vector Backbone Description: Backbone Marker:Jay Shendure, JB Lalanne, Sam Regalado; Vector Backbone:p051_PB_tornado; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40631108
Comments: A FISSEQ capable barcode must first be cloned into this plasmid via BsmBI/Esp3I and a gene or ORF library must be cloned in via SapI before transfection. This vector must be co-transfected with another vector containing the piggybac transposase in order to integrate into the genome.
Please visit https://www.biorxiv.org/content/10.1101/2025.07.03.663081v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_252243 Copy
Vector Backbone Description: Backbone Marker:Jay Shendure, JB Lalanne, Sam Regalado; Vector Backbone:p051_PB_tornado; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40631108
Comments: A FISSEQ capable barcode must first be cloned into this plasmid via BsmBI/Esp3I and a gene or ORF library must be cloned in via SapI before transfection. This vector must be co-transfected with another vector containing the piggybac transposase in order to integrate into the genome.
Please visit https://www.biorxiv.org/content/10.1101/2025.07.03.663081v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_252244 Copy
Vector Backbone Description: Backbone Marker:Jay Shendure, JB Lalanne, Sam Regalado; Vector Backbone:p051_PB_tornado; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40631108
Comments: A FISSEQ capable barcode must first be cloned into this plasmid via BsmBI/Esp3I and a gene or ORF library must be cloned in via SapI before transfection. This vector must be co-transfected with another vector containing the piggybac transposase in order to integrate into the genome.
Please visit https://www.biorxiv.org/content/10.1101/2025.07.03.663081v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_252245 Copy
Species: Synthetic
Genetic Insert: st-CoChR-GP2A-eGFP
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_253382 Copy
Species: SARS-CoV-2
Genetic Insert: CoV-2_frD ΔS/GFP
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37988285
Comments: detailed explanation of CLEVER reverse genetics methodology in DOI: 10.7554/eLife.89035
Proper citation: RRID:Addgene_253144 Copy
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