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Vector Backbone Description: Vector Backbone:pBAtC; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36456803
Proper citation: RRID:Addgene_189640 Copy
Species: Synthetic
Genetic Insert: PURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35173283
Proper citation: RRID:Addgene_185840 Copy
Species: Other
Genetic Insert: Cas9-2A-Puro
Vector Backbone Description: Vector Backbone:modRNA cap0 plasmid; Vector Types:IVT template plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36160051
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.05.24.493220v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172855 Copy
Species: Aedes aegypti
Genetic Insert: AAEL003877-dCAS9-VPR
Vector Backbone Description: Backbone Size:5246; Vector Backbone:Plasmid #100581; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36656895
Proper citation: RRID:Addgene_183993 Copy
Species: Synthetic
Genetic Insert: see comments
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35610221
Comments: PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PGAL1>ERG20F96C>TEBS1-PSk.GAL2>Xd.CRtYBE83K>TCYC1-PSe.GAL2>XdCrtI>TRPL41B-PBTS1> RPL25(partial; Arm2)
Proper citation: RRID:Addgene_185890 Copy
Species: Homo sapiens
Genetic Insert: dimeric MBD1
Vector Backbone Description: Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34586782
Proper citation: RRID:Addgene_186462 Copy
Species: Streptomyces coelicolor M145
Genetic Insert: scbA
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pBbA2k; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29510026
Proper citation: RRID:Addgene_186628 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmex-5::Cas9, Cbr-unc-119
Vector Backbone Description: Vector Backbone:pMLS621; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34748534
Proper citation: RRID:Addgene_188892 Copy
Species: Homo sapiens
Genetic Insert: GPa3b17 hTCR alpha
Vector Backbone Description: Vector Backbone:pHRi; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35985289
Proper citation: RRID:Addgene_187601 Copy
Species: Oryza sativa
Genetic Insert: OsCYP711A2
Vector Backbone Description: Backbone Size:6544; Vector Backbone:pAG416-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34533983
Proper citation: RRID:Addgene_178301 Copy
Species: Oryza sativa
Genetic Insert: OsCYP711A3
Vector Backbone Description: Backbone Marker:Susan Lindquist; Backbone Size:7387; Vector Backbone:pAG414GPD-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34533983
Proper citation: RRID:Addgene_178300 Copy
Species: Homo sapiens
Genetic Insert: 1G4 hTCR beta, and SNAP tag
Vector Backbone Description: Vector Backbone:pHRi; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35985289
Proper citation: RRID:Addgene_187604 Copy
Species: Homo sapiens
Genetic Insert: 1G4 hTCR alpha
Vector Backbone Description: Vector Backbone:pHRi; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35985289
Proper citation: RRID:Addgene_187603 Copy
Species: Homo sapiens
Genetic Insert: GPa3b17 hTCR beta
Vector Backbone Description: Vector Backbone:pHRi; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35985289
Proper citation: RRID:Addgene_187602 Copy
Species: Fragaria vesca
Genetic Insert: FvCYP722C2
Vector Backbone Description: Backbone Marker:Susan Lindquist; Backbone Size:7387; Vector Backbone:pAG414GPD-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34533983
Proper citation: RRID:Addgene_178299 Copy
Vector Backbone Description: Backbone Size:6440; Vector Backbone:pUC19; Vector Types:Expression vector for targeted integration; Bacterial Resistance:Apramycin
Defining Citation: PMID:34890250
Comments: For cloning in homology arms, can use Gateway, restriction ligation, or homology-based cloning such as Gibson/Infusion cloning. eGFP can be swapped for your gene of interest or can be fused (recommend homology base cloning).
Original pSelAct backbone was developed here:
Geize, R. van der, Jong, W. de, Hessels, G. I., Grommen, A. W. F., Jacobs, A. A. C., and Dijkhuizen, L. 2008. A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality. Nucleic Acids Res. 36:e151
pSelAct-Express was develop and tested here:
Stevens, D. M., Tang, A., and Coaker, G. 2021. A Genetic Toolkit for Investigating Clavibacter Species: Markerless Deletion, Permissive Site Identification, and an Integrative Plasmid. Mol Plant-microbe Interactions. 34:1336–1345
Proper citation: RRID:Addgene_174375 Copy
Species: Vigna unguiculata
Genetic Insert: VuCYP722C
Vector Backbone Description: Backbone Marker:Susan Lindquist; Backbone Size:7387; Vector Backbone:pAG414GPD-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34533983
Proper citation: RRID:Addgene_178293 Copy
Species: Lotus japonicus
Genetic Insert: LjCYP722C
Vector Backbone Description: Backbone Marker:Susan Lindquist; Backbone Size:7387; Vector Backbone:pAG414GPD-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34533983
Proper citation: RRID:Addgene_178292 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:R6Kgamma; Vector Types:Bacterial Expression; Bacterial Resistance:Amp + Kan + Dap
Comments: This plasmid requires a pir+ strain for growth and transformation. It will be distributed in MFDpir E. coli. MFDpir strain is auxotrophic for DAP (diaminopimelic acid), so it must be added to the media to grow and extract the plasmid (0.3 mM working concentration).
Proper citation: RRID:Addgene_187382 Copy
Species: Mus musculus
Genetic Insert: mouse Talin 1 F2F3 P229L mutant
Vector Backbone Description: Backbone Size:5742; Vector Backbone:pET151; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35861643
Proper citation: RRID:Addgene_177879 Copy
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