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Species: Caenorhabditis elegans
Genetic Insert: PU6(R07E5.16)::Ric-4 sgRNA
Vector Backbone Description: Vector Backbone:pBluescript II KS+; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34748534
Proper citation: RRID:Addgene_188891 Copy
Vector Backbone Description: Vector Backbone:Unspecified; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36001082
Proper citation: RRID:Addgene_187890 Copy
Genetic Insert: Gab1(PRD) CluMPS
Vector Backbone Description: Backbone Size:3948; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38335954
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.07.13.499962v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_190026 Copy
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35610221
Comments: PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2-TRPL25(Arm 3)- ARS305-PTEF1> yEGFP> TURA3- PERG1> RPL25(partial; Arm2)
Proper citation: RRID:Addgene_185873 Copy
Species: Synthetic
Genetic Insert: SpCas9_H840A_KK_SpG-MMLV-RT_co-P2A-PAC_dTK
Vector Backbone Description: Backbone Marker:Eurofins Genomics; Backbone Size:5120; Vector Backbone:pEX-A2; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology, piggyBac transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34773059
Proper citation: RRID:Addgene_187895 Copy
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35610221
Comments: PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2-TRPL25(Arm 3)- ARS305-PTEF1> yEGFP> TURA3
Proper citation: RRID:Addgene_185871 Copy
Species: Homo sapiens
Genetic Insert: INSR
Vector Backbone Description: Vector Backbone:MAC-tag-C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35384245
Proper citation: RRID:Addgene_187772 Copy
Species: M13 bacteriophage
Genetic Insert: M13 gene III
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pLITMUS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27982027
Comments: Addgene QC NGS identifies a SNP at nucleotide 1471 in the M13 ori. It is unknown what, if any, impact this may have on plasmid function.
Proper citation: RRID:Addgene_80908 Copy
Species: 229E
Genetic Insert: 229E spike
Vector Backbone Description: Backbone Size:4200; Vector Backbone:phCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_188908 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15223344
Proper citation: RRID:Addgene_186706 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27980067
Proper citation: RRID:Addgene_186705 Copy
Species: Gallus gallus
Genetic Insert: paxillin-Electra2
Vector Backbone Description: Backbone Size:3933; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35715437
Proper citation: RRID:Addgene_184926 Copy
Species: Homo sapiens
Genetic Insert: PKMYT1-V5-TurboID
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_135247 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:modRNA cap0 plasmid; Vector Types:IVT template plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36160051
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.05.24.493220v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_170180 Copy
Species: Synthetic
Genetic Insert: Electra2-P2A-EGFP
Vector Backbone Description: Backbone Size:4723; Vector Backbone:pAAV-CAG-EGFP-WPRE; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35715437
Proper citation: RRID:Addgene_184936 Copy
Species: Synthetic
Genetic Insert: ALD(-125, 40)- ALD6(1054, 1749)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34499421
Proper citation: RRID:Addgene_185866 Copy
Species: MS2 Phage
Genetic Insert: Maturation Protein
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:2353; Vector Backbone:pMX; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36644571
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.09.04.506540 for bioRxiv preprint.
Proper citation: RRID:Addgene_179156 Copy
Species: Synthetic
Genetic Insert: EBFP2-P2A-EGFP
Vector Backbone Description: Backbone Size:4723; Vector Backbone:pAAV-CAG-EGFP-WPRE; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35715437
Proper citation: RRID:Addgene_184938 Copy
Species: Synthetic
Genetic Insert: mClover3
Vector Backbone Description: Vector Backbone:pGRG36; Vector Types:Bacterial Expression, miniTn7 delivery vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30631309
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.
Proper citation: RRID:Addgene_118555 Copy
Species: Homo sapiens
Genetic Insert: 1G4 TCR alpha
Vector Backbone Description: Vector Backbone:pHRSin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35985289
Proper citation: RRID:Addgene_187572 Copy
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