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Species: Homo sapiens
Genetic Insert: DDX28 pre sequence
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4011; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42026137
Comments: Please visit https://doi.org/10.1101/2025.06.06.658068 for bioRxiv preprint.
Proper citation: RRID:Addgene_254615 Copy
Species: Synthetic
Genetic Insert: bpNLS-Stab-exin21-eeBxb1-Stab
Vector Backbone Description: Backbone Marker:Addgene #139987; Vector Backbone:pCMV-T7-SpCas9-P2A-EGFP (RTW3027); Vector Types:Mammalian Expression, CRISPR, in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41813887
Proper citation: RRID:Addgene_248212 Copy
Species: Homo sapiens
Genetic Insert: DDX28
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4011; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42026137
Comments: Please visit https://doi.org/10.1101/2025.06.06.658068 for bioRxiv preprint.
Proper citation: RRID:Addgene_254616 Copy
Species: Synthetic
Genetic Insert: pNG2 ORI plus NGS barcode 22, chloramphenicol resistance cassette, mScarlet-I cassette, oriT
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: Please note that this plasmid may run as a dimer or higher multimer.
Proper citation: RRID:Addgene_251860 Copy
Species: Homo sapiens
Genetic Insert: HDAC8
Vector Backbone Description: Vector Backbone:pBOBI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39695235
Proper citation: RRID:Addgene_246157 Copy
Vector Backbone Description: Backbone Size:14937; Vector Backbone:pRS159; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42115739
Comments: Please visit https://doi.org/10.64898/2025.12.19.695556 for bioRxiv preprint.
Proper citation: RRID:Addgene_250493 Copy
Species: Salmonella enterica
Genetic Insert: SeAbpA, SeAbpB
Vector Backbone Description: Vector Backbone:pBAD02; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_254574 Copy
Species: Sinorhizobium meliloti
Genetic Insert: SmHna
Vector Backbone Description: Vector Backbone:pBAD02; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_254576 Copy
Species: Synthetic
Genetic Insert: pBC1 ORI plus NGS barcode 25, erythromycin resistance cassette, mScarlet-I cassette, oriT
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Erythromycin
Comments: Please note that this plasmid may run as a dimer or higher multimer.
Proper citation: RRID:Addgene_251869 Copy
Species: Synthetic
Genetic Insert: pSa ORI plus NGS barcode 16, erythromycin resistance cassette, mScarlet-I cassette, oriT
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Erythromycin
Comments: Please note that this plasmid may run as a dimer or higher multimer.
Proper citation: RRID:Addgene_251868 Copy
Species: Solibacillus silvestris StLB046
Genetic Insert: SsNsnA, SsNsnB, SsNsnC
Vector Backbone Description: Vector Backbone:pBAD02; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_254577 Copy
Species: Homo sapiens
Genetic Insert: LIMD1
Vector Backbone Description: Vector Backbone:pBOBI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26215701
Proper citation: RRID:Addgene_246205 Copy
Species: Homo sapiens
Genetic Insert: EGLN1
Vector Backbone Description: Vector Backbone:pBOBI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26215701
Proper citation: RRID:Addgene_246209 Copy
Species: Synthetic
Genetic Insert: ccdb
Vector Backbone Description: Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://doi.org/10.1101/2025.08.05.668824 for bioRxiv preprint.
Proper citation: RRID:Addgene_247379 Copy
Species: Homo sapiens
Genetic Insert: RhoBTB3
Vector Backbone Description: Vector Backbone:pcDNA 3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26215701
Proper citation: RRID:Addgene_246208 Copy
Species: Homo sapiens
Genetic Insert: LIMD1
Vector Backbone Description: Vector Backbone:pBOBI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26215701
Proper citation: RRID:Addgene_246206 Copy
Species: Synthetic
Genetic Insert: arsR, menD
Vector Backbone Description: Vector Backbone:p15A ori_cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Please visit https://doi.org/10.1101/2025.09.24.678173 for bioRxiv preprint.
Proper citation: RRID:Addgene_252937 Copy
Species: Synthetic
Genetic Insert: GCaMP8m
Vector Backbone Description: Vector Backbone:pAAV-Ef1a-FLEx(FRT); Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42162422
Comments: Please visit https://doi.org/10.21203/rs.3.rs-6831193/v1 for preprint.
Proper citation: RRID:Addgene_252530 Copy
Species: Synthetic
Genetic Insert: gRNA_LP400~3p
Vector Backbone Description: Backbone Size:8500; Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41890105
Comments: Plasmid express a gRNA driven by a human U6 promoter and a CAG promoter-driven SpCas9-NLS.
Proper citation: RRID:Addgene_254039 Copy
Genetic Insert: GFP_AID*
Vector Backbone Description: Backbone Marker:Andreas Milias-Argeitis, Addgene Plasmid #180285 as described by Novarina et al. (2022).; Vector Backbone:pYTK-DN4; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Comments: The golden gate (Multi-gene) plasmid assembly was performed according to the MoClo-Cloning standards described in: Lee, M. E., DeLoache, W. C., Cervantes, B., & Dueber, J. E. (2015). A highly characterized yeast toolkit for modular, multipart assembly. ACS synthetic biology, 4(9), 975-986.
Parts from the MoClo Yeast Toolkit (Addgene kit #1000000061) were used for MoClo assembly.
The OsTIR1(F74G, S210A) variant was originally described in Xing et al., 2025 (https://doi.org/10.1038/s41467-025-61848-1).
Proper citation: RRID:Addgene_253756 Copy
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