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Vector Backbone Description: Vector Backbone:JD633; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: Please visit https://doi.org/10.64898/2026.02.10.705172 for bioRxiv preprint.
The reference articles to construct this plasmid:
A GRF-GIF chimeric protein improves the regeneration efficiency of transgenic plants. Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik JF, Dubcovsky J. Nat Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 10.1038/s41587-020-0703-0. 10.1038/s41587-020-0703-0 PubMed 33046875
A reporter for noninvasively monitoring gene expression and plant transformation. He Y, Zhang T, Sun H, Zhan H, Zhao Y. Hortic Res. 2020 Sep 19;7:152. doi: 10.1038/s41438-020-00390-1. eCollection 2020. 10.1038/s41438-020-00390-1 PubMed 33024566
Proper citation: RRID:Addgene_253103 Copy
Species: Triticum aestivum and Triticum turgidum
Genetic Insert: SpCas9-P2A-RUBY
Vector Backbone Description: Vector Backbone:JD633; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: Please visit https://doi.org/10.64898/2026.02.10.705172 for bioRxiv preprint.
The reference articles to construct this plasmid:
A GRF-GIF chimeric protein improves the regeneration efficiency of transgenic plants. Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik JF, Dubcovsky J. Nat Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 10.1038/s41587-020-0703-0. 10.1038/s41587-020-0703-0 PubMed 33046875
A reporter for noninvasively monitoring gene expression and plant transformation. He Y, Zhang T, Sun H, Zhan H, Zhao Y. Hortic Res. 2020 Sep 19;7:152. doi: 10.1038/s41438-020-00390-1. eCollection 2020. 10.1038/s41438-020-00390-1 PubMed 33024566
Proper citation: RRID:Addgene_253104 Copy
Species: Synthetic
Genetic Insert: nuc-diGlo
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42564856
Comments: Please visit https://doi.org/10.64898/2026.01.15.699810 for bioRxiv preprint.
Proper citation: RRID:Addgene_251843 Copy
Species: Escherichia coli
Genetic Insert: EcHna
Vector Backbone Description: Vector Backbone:pBAD02; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_254560 Copy
Species: Escherichia coli strain UMB0934
Genetic Insert: EcPD(D/E)XK nuclease
Vector Backbone Description: Vector Backbone:pBAD02; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_254565 Copy
Species: Escherichia coli MOD1-ECOR58
Genetic Insert: EcP-loop NTPase, EcPD(D/E)XK nuclease
Vector Backbone Description: Vector Backbone:pBAD02; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_254566 Copy
Species: Synthetic
Genetic Insert: MagLOV 2
Vector Backbone Description: Backbone Size:2314; Vector Backbone:pTU1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41565820
Proper citation: RRID:Addgene_252303 Copy
Species: Homo sapiens
Genetic Insert: ZC3H12A
Vector Backbone Description: Backbone Marker:Kosuke Yusa; Backbone Size:8648; Vector Backbone:pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_252912 Copy
Species: HPV
Genetic Insert: HPV 16E6
Vector Backbone Description: Backbone Size:5874; Vector Backbone:pLXSN16E6; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41910269
Proper citation: RRID:Addgene_253284 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUMO family protein SMT3
Vector Backbone Description: Backbone Size:5228; Vector Backbone:pET29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41927759
Comments: This plasmid contains a SUMO protein construct to serve as a control protein for binding experiments using the AH-SUMO platform, to verify non-interaction between the SUMO domain and liposomes of choice. It contains the SUMO1-98 region of the SMT3_yeast gene, followed by a Cysteine, a TEV site and a hexa-histidine site. On the N-terminal of the SUMO domain, it contains a region encoding for MGSGS, which is there to mimic the structure of a AH-SUMO platform that contains an N-terminal peptide of interest , serving as a better control.
Please visit https://doi.org/10.1101/2025.05.12.653516 for the bioRxiv preprint.
Proper citation: RRID:Addgene_254139 Copy
Species: Synthetic
Genetic Insert: cyto-iGlo
Vector Backbone Description: Backbone Size:5422; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42564856
Comments: Please visit https://doi.org/10.64898/2026.01.15.699810 for bioRxiv preprint.
Proper citation: RRID:Addgene_251830 Copy
Species: Albibacillus kandeliae
Genetic Insert: AkDUF1887
Vector Backbone Description: Vector Backbone:pBAD02; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_254549 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Leaf compost cutinase
Vector Backbone Description: Backbone Marker:Paul Young, Addgene plasmid #212923; Vector Backbone:pYSD099; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253050 Copy
Species: Synthetic
Genetic Insert: YFP
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40246839
Proper citation: RRID:Addgene_253970 Copy
Species: Homo sapiens
Genetic Insert: mRuby3-diGlo
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42564856
Comments: Please visit https://doi.org/10.64898/2026.01.15.699810 for bioRxiv preprint.
Proper citation: RRID:Addgene_251835 Copy
Species: Unspecified
Genetic Insert: L4-spacer-RE
Vector Backbone Description: Vector Backbone:Unspecified; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41535170
Proper citation: RRID:Addgene_254028 Copy
Species: Synthetic
Genetic Insert: matrix-iGlo
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42564856
Comments: Please visit https://doi.org/10.64898/2026.01.15.699810 for bioRxiv preprint.
Proper citation: RRID:Addgene_251832 Copy
Species: Acinetobacter baumannii
Genetic Insert: mltB
Vector Backbone Description: Vector Backbone:pETAb; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_253611 Copy
Species: Synthetic
Genetic Insert: cyto-diGlo
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42564856
Comments: Please visit https://doi.org/10.64898/2026.01.15.699810 for bioRxiv preprint.
Proper citation: RRID:Addgene_251831 Copy
Species: Aequorea victoria
Genetic Insert: Protein A
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252975 Copy
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