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Species: Prevotella intermedia
Genetic Insert: Pin2Cas13
Vector Backbone Description: Backbone Size:6087; Vector Backbone:SUMO bacterial expression vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29449508
Proper citation: RRID:Addgene_115216 Copy
Species: Homo sapiens
Genetic Insert: PARK7E163K
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:2790; Vector Backbone:pENTR223; Vector Types:gateway cloning into appropriate destination vectors; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31536960
Comments: Derived from HsCD00383508 from Harvard PlasmidID. Mutagenesis performed using overlap-extension PCR in combination with Restriction digestion/ligation.
Proper citation: RRID:Addgene_115181 Copy
Species: Bergeyella zoohelcum
Genetic Insert: BzoCas13
Vector Backbone Description: Backbone Size:6087; Vector Backbone:SUMO bacterial expression vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29449508
Proper citation: RRID:Addgene_115207 Copy
Species: Prevotella intermedia
Genetic Insert: PinCas13
Vector Backbone Description: Backbone Size:6087; Vector Backbone:SUMO bacterial expression vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29449508
Proper citation: RRID:Addgene_115208 Copy
Species: Homo sapiens
Genetic Insert: CR (CRISPR/Cas9-resistant)-PDHA2S291A
Vector Backbone Description: Backbone Marker:David Root, Addgene plasmid # 41392; Backbone Size:8270; Vector Backbone:pLEX-307; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31536960
Proper citation: RRID:Addgene_115203 Copy
Species: Homo sapiens
Genetic Insert: CR (CRISPR/Cas9-resistant)-PDHA2S293A
Vector Backbone Description: Backbone Marker:David Root, Addgene plasmid # 41392; Backbone Size:8270; Vector Backbone:pLEX-307; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31536960
Proper citation: RRID:Addgene_115204 Copy
Species: Homo sapiens
Genetic Insert: CHMP1B
Vector Backbone Description: Vector Backbone:pCA528; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32251413
Proper citation: RRID:Addgene_115325 Copy
Species: Synthetic
Genetic Insert: TrxRFP1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pBAD/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28671680
Proper citation: RRID:Addgene_115327 Copy
Species: Mus musculus
Genetic Insert: Sema4A
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29981480
Proper citation: RRID:Addgene_115171 Copy
Species: Enterococcus italicus
Genetic Insert: EiCsm6
Vector Backbone Description: Backbone Size:6084; Vector Backbone:SUMO bacterial expression vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29449508
Proper citation: RRID:Addgene_115268 Copy
Species: B. subtilis
Genetic Insert: phosphotransacetylase
Vector Backbone Description: Backbone Size:7313; Vector Backbone:pLIC.B4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16283428
Comments: Please note that the plasmid pLIC.B4.BSU37660 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
Proper citation: RRID:Addgene_11535 Copy
Species: S. pyogenes
Genetic Insert: IscU
Vector Backbone Description: Backbone Size:7313; Vector Backbone:pLIC.B4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15815978
Comments: Please note that the plasmid pLIC.B4.SPy0289 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
Proper citation: RRID:Addgene_11534 Copy
Species: Homo sapiens
Genetic Insert: SOX10
Vector Backbone Description: Backbone Size:9100; Vector Backbone:pZ P TetON-3XFLAG-tdT CAGG-m2rtTA v2 (AddGene 112668); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29337119
Proper citation: RRID:Addgene_115240 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSP104
Vector Backbone Description: Backbone Size:4895; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14978213
Proper citation: RRID:Addgene_1153 Copy
Species: Homo sapiens
Genetic Insert: Rac1 5'-UTR
Vector Backbone Description: Vector Backbone:pFL-SV40; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30373933
Proper citation: RRID:Addgene_115354 Copy
Species: Synthetic
Genetic Insert: mTagBFP2 and mCherry
Vector Backbone Description: Backbone Marker:Inder Verma Lab; Vector Backbone:pCSC-SP-PW-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37053554
Proper citation: RRID:Addgene_115234 Copy
Species: Synthetic
Genetic Insert: mTagBFP2 and mCherry
Vector Backbone Description: Backbone Marker:Inder Verma Lab; Vector Backbone:pCSC-SP-PW-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37053554
Proper citation: RRID:Addgene_115235 Copy
Species: Homo sapiens
Genetic Insert: Rab7a fused to mEmerald
Vector Backbone Description: Backbone Marker:Chung K-H et al., Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR 2006 vol 24; Backbone Size:5235; Vector Backbone:pAAV-SIBR-anti_I; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_115238 Copy
Species: Homo sapiens
Genetic Insert: sgSNAI1-2097
Vector Backbone Description: Backbone Marker:Broad Institute - Genetic Perturbation Platform; Vector Backbone:pXPR_BRD003; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30059005
Proper citation: RRID:Addgene_115453 Copy
Species: Homo sapiens
Genetic Insert: shLacZ-1735
Vector Backbone Description: Backbone Marker:Broad Institute - Genetic Perturbation Platform; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30059005
Proper citation: RRID:Addgene_115454 Copy
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