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Species: Saccharomyces cerevisiae
Genetic Insert: RNA Polymerase II CTD
Vector Backbone Description: Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9468530
Proper citation: RRID:Addgene_160688 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ull1 delta440
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12761287
Proper citation: RRID:Addgene_16087 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Medium-chain fatty acid ethyl ester synthase
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32886882
Proper citation: RRID:Addgene_160864 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SMT3
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-KG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11577116
Proper citation: RRID:Addgene_16092 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: UBC9
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-KG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12761287
Proper citation: RRID:Addgene_16090 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pol1
Vector Backbone Description: Vector Backbone:pGST-parallel; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32923642
Proper citation: RRID:Addgene_160990 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VRG4
Vector Backbone Description: Backbone Size:4000; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30858194
Comments: Parental strain JK93da.
Proper citation: RRID:Addgene_160904 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pol1
Vector Backbone Description: Vector Backbone:pGST-parallel; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32923642
Proper citation: RRID:Addgene_160988 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MCP-mCherry-HIS tag, 3'UTR MS2 Stem Loop
Vector Backbone Description: Backbone Size:6000; Vector Backbone:plIVMD156; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037152
Proper citation: RRID:Addgene_160935 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MCP-sfGFP-HIS tag, 3'UTR MS2 Stem Loop
Vector Backbone Description: Backbone Size:6000; Vector Backbone:plIVMD156; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037152
Comments: Please note that the T247A mutation in sfGFP does not impact plasmid function.
Proper citation: RRID:Addgene_160936 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MCP-mTagBFP2-HA Tag
Vector Backbone Description: Vector Backbone:psh100; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037152
Proper citation: RRID:Addgene_160939 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HHF1
Vector Backbone Description: Backbone Marker:Berkeley Macrolab; Backbone Size:4786; Vector Backbone:pET (https://www.addgene.org/29666/); Vector Types:; Bacterial Resistance:Ampicillin
Comments: This is an improved version of the plasmid reported here:
https://www.sciencedirect.com/science/article/abs/pii/S096921261930440X
https://doi.org/10.1016/j.str.2019.12.002
All histone genes code for S. cerevisiae proteins. Expression and purification procedures are as described.
Proper citation: RRID:Addgene_160930 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HHF1
Vector Backbone Description: Backbone Marker:Berkeley Macrolab; Backbone Size:4786; Vector Backbone:pET (https://www.addgene.org/29666/); Vector Types:; Bacterial Resistance:Ampicillin
Comments: This is an improved version of the plasmid reported here:
https://www.sciencedirect.com/science/article/abs/pii/S096921261930440X
https://doi.org/10.1016/j.str.2019.12.002
All histone genes code for S. cerevisiae proteins.
Proper citation: RRID:Addgene_160929 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Nop4 delta E5
Vector Backbone Description: Vector Backbone:p414GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33941690
Proper citation: RRID:Addgene_169263 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL2
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrXII:291472..291491 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166088 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CUP1-1
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrVIII:212613..212632 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166086 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Promoter of RPL22A
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrXII:263006..263025 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome.
Addgene QC identified discrepancies compared to the theoretical sequence. The depositing lab confirms the functional activity of the construct despite these differences.
Proper citation: RRID:Addgene_166079 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Promoter of RPL39 (Overlaps with 5'UTR and first base of gene)
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrX: 75914-75933 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166078 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SLA1
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrII: 213734-213753 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166075 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Promoter of RRT8
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrXV:241445..241464 (-strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166080 Copy
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