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Species: Xenopus laevis
Genetic Insert: Xfin fragment
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2417; Vector Backbone:pSP70; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2826129
Comments: See author's map for more information.
Proper citation: RRID:Addgene_15053 Copy
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCMV Sport 6 with U6 promoter; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16426460
Comments: The pCS6/U6 vector was made by ligating an M13 PCR product from the pBS/U6 vector (from the Shi lab, Harvard Medical School) to a Rsr II-Mlu I blunted pCMV-Sport6 vector (Invitrogen). This vector is compatible with Invitrogen's Gateway system.
See Author's Map for cloning information and the article for a protocol for cloning hairpins into this vector (in the article's supplementary material).
Proper citation: RRID:Addgene_15173 Copy
Genetic Insert: Gateway cassette
Vector Backbone Description: Backbone Size:12000; Vector Backbone:RCAS (A); Vector Types:Retroviral, RNAi, Avian expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16426460
Comments: The RCAS-RNAi viruses have been modified so that they are now compatible with the Gateway cloning system (Invitrogen). DNA encoding the hairpin is cloned into a modified U6 vector using standard cloning techniques. The construct is then moved into the retroviral vector using Gateway reagents, thus bypassing the difficulties of cloning small fragments into RCAS. As a result, each construct can be made with a minimal number of cloning steps. See Supplemental Information of the article for a detailed protocol.
Proper citation: RRID:Addgene_15182 Copy
Species: Mus musculus
Genetic Insert: beta2-YFP-M
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14684858
Comments: YFP with an upstream HA epitope tag was inserted into PpuMI restriction site of the (M3-M4) loop at residue 381 (beta2-YFP-M).
Proper citation: RRID:Addgene_15107 Copy
Species: Xenopus laevis
Genetic Insert: BMP receptor
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSP64TNE; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7522972
Comments: To make mRNA for the full length BMP receptor. The clone contains no untranslated sequences.
Proper citation: RRID:Addgene_15068 Copy
Species: Homo sapiens
Genetic Insert: Dsh2
Vector Backbone Description: Backbone Marker:Moon Lab; Backbone Size:5500; Vector Backbone:pIRESpuro-GLUE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16547521
Proper citation: RRID:Addgene_15101 Copy
Species: Xenopus laevis
Genetic Insert: BMP receptor
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSP64T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7522972
Comments: To make RNA for truncated dominant negative form of RU1. The truncation is at one amino acid past the transmembrane domain.
Proper citation: RRID:Addgene_15067 Copy
Species: Xenopus laevis
Genetic Insert: BMP2-Vg1
Vector Backbone Description: Backbone Marker:Modified form of pSP64T that contains KpnI & XhoI near BglII; Backbone Size:3000; Vector Backbone:pSP64T3; Vector Types:Sp6 expression for use in Xenopus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8348610
Comments: PCR amplified Pro domain of Xenopus BMP2 including tetrabasic cleavage site and 4 a.a. downstream, fused to PCR fragment of Vg1 ligand domain (without first 3 a.a.).
See article for more cloning details.
The plasmid was linearized with EcoRI prior to in vitro synthesis of synthetic capped mRNA.
Proper citation: RRID:Addgene_15071 Copy
Species: Mus musculus
Genetic Insert: p27
Vector Backbone Description: Backbone Marker:Cepko Lab; Backbone Size:0; Vector Backbone:pLIA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11404411
Proper citation: RRID:Addgene_15191 Copy
Species: Synthetic
Genetic Insert: sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
Vector Backbone Description: Vector Backbone:pUCIDT; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32231336
Proper citation: RRID:Addgene_140096 Copy
Species: Sinomenium acutum
Genetic Insert: Dechloroacutumine halogenase G226D variant from Sinomenium acutum
Vector Backbone Description: Backbone Size:5276; Vector Backbone:pHis8-4b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32313070
Comments: Expressed robustly in E.coli. Catalyzes hydroxylation of dechloroacutumine in vitro
Proper citation: RRID:Addgene_140130 Copy
Species: Mus musculus
Genetic Insert: Wnt1
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Comments: Contains an internal Cla I site, from Javier Capdevila.
Proper citation: RRID:Addgene_14017 Copy
Species: Synthetic
Genetic Insert: NCreV
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32203389
Comments: Funded by the BRAIN Initiative.
Proper citation: RRID:Addgene_140131 Copy
Species: Homo sapiens
Genetic Insert: gRNA1+scaffold+H1promoter+gRNA2
Vector Backbone Description: Vector Backbone:pDECKO_mCherry; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35619054
Proper citation: RRID:Addgene_140099 Copy
Species: Gallus gallus
Genetic Insert: Slug
Vector Backbone Description: Backbone Size:3000; Vector Backbone:Slax13; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Full length coding sequence, PCR (vent) product, 5’ primer converts 2nd codon from cca (pro) to gca (ala) in order to generate a Nco I site; 3’ primer is wt plus EcoRI site, from Malcolm Logan.
Proper citation: RRID:Addgene_14015 Copy
Species: Gallus gallus
Genetic Insert: HoxD10
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Full-length Hox D10, from Craig Nelson.
Proper citation: RRID:Addgene_14010 Copy
Species: Other
Genetic Insert: FlaviA-mNeptune
Vector Backbone Description: Backbone Marker:Eric Campeau, Paul Kaufman; Backbone Size:7871; Vector Backbone:pLenti CMV GFP Puro (658-5) (Addgene Plasmid #17448); Vector Types:Mammalian Expression, Lentiviral, High expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31919100
Comments: The FlaviA-mNeptune reporter is not suitable for transient transfection as the reporter has a basal background that makes it sensitive to the levels of expression: If the expression is too high the background will mask the signal produced by the reporter and/or generate OSER structures - If the expression is too low you won’t detect enough signal over the background. Our recommendation is to use the pLenti-CMV-FlaviA-mNeptune-puro construct to pack lentiviral particles with the psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) plasmids, generate stable cell lines and sort cell subpopulations with different levels of the reporter's basal background before testing them. The best reporter cells will be those with the highest signal-to-noise ratio upon flavivirus infection.
Proper citation: RRID:Addgene_140091 Copy
Species: Synthetic
Genetic Insert: iFlpV
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32203389
Comments: Funded by the BRAIN Initiative.
Proper citation: RRID:Addgene_140137 Copy
Genetic Insert: lacY
Vector Backbone Description: Backbone Size:4938; Vector Backbone:pAN1818; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32355194
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_140139 Copy
Species: Synthetic
Genetic Insert: Stabilized hairpin of miR-let7d
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28525643
Comments: let7d_stbC motif was inserted between BamHI and AgeI sites of pAptamerCassette-EGFP (ID: 140288).
Proper citation: RRID:Addgene_140266 Copy
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