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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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Ngn3 Cre-ER (DM#256) Resource Report Resource Website |
RRID:Addgene_15024 | Ngn3 promoter | Mus musculus | Ampicillin | PMID:11973276 | The CRE-ER(TM) protein requires tamoxifen (TM) to catalyze LoxP site-mediated excision. To generate the Ngn3 constructs, a 7.6 kb fragment containing Ngn3 (pGD15, contains the 6.5 kb upstream region of Ngn3) was isolated using Ngn3 cDNA to screen a mouse RPI-22 BAC library (Genetics Institute, Buffalo, NY). Three primers were used to fuse the Cre-ERTM-coding region to the Ngn3 ATG to generate a fragment that had part of the Ngn3 promoter and the 5' end of the recombinase. The primers used are p73 (5'-acacttgactccttgatcgctg-3'), p71 (5'-gttgcatcgaccggtaatgca-3') and p74 (5'-ttttcccaaccgcaggatgtccaatttactgaccgtacac-3'). This fragment was subsequently digested with ApaI and AgeI and ligated to the KpnI (blunt-ended)-ApaI (partial digestion)-digested pGD15, and a 2.4 kb AgeI-SacII (partial digestion, blunt-ended) fragment of pGD35. The insert from the final construct (pGD29) was released by NotI digestion. See Author's Map and article for more information. | Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, mouse transgenic; Bacterial Resistance:Ampicillin | 2026-09-05 12:49:31 | 0 | |
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Ngn3 Cre (DM#259) Resource Report Resource Website |
RRID:Addgene_15023 | Ngn3 promoter | Mus musculus | Ampicillin | PMID:11973276 | To generate the Ngn3 constructs, a 7.6 kb fragment containing Ngn3 (pGD15, contains the 6.5 kb upstream region of Ngn3) was isolated using Ngn3 cDNA to screen a mouse RPI-22 BAC library. The Ngn3-Cre construct was obtained by directly ligating a XbaI-BssHII (partial digestion) blunt-ended fragment from pGD15 to the pMC-Cre plasmid to generate pGD 84. The insert was released by NotI-SalI digestion and used for transgenic animal production. See Author's Map and article for more information. | Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, mouse transgenic; Bacterial Resistance:Ampicillin | 2026-09-05 12:49:31 | 0 | |
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Pdx1 Cre-ER (DM#255) Resource Report Resource Website |
RRID:Addgene_15022 | Pdx1 promoter | Mus musculus | Ampicillin | PMID:11973276 | The CRE-ER(TM) protein requires tamoxifen (TM) to catalyze LoxP site-mediated excision. For Pdx-1-Cre-ER(TM), the coding region of the Cre-ER(TM) cDNA was directly fused to the starting ATG of the PDX1 protein by PCR. Three primers (p5, 5'-ttgaaacaagtgcaggtgttcg-3'; p75, 5'-gttgcatcgaccggtaatgcaggcaaattttggtgtacggtcagtaaattggacatggtggcagccggcact-3'; and p71, 5'-gttgcatcgaccggtaatgca-3') were used. First, p5 and p75 were used to amplify a 400 base pair fragment from the Pdx1 genomic DNA. Then this fragment was used together with p5 and p71 and the Cre-ER(TM) plasmid to obtain a fragment that has the 5' end coding region directly fused to the Pdx1 promoter. This fragment was digested with AgeI and ligated to the XhoI (blunt-ended)-AgeI double-digested pBSCre-ER(TM) that contains the full-length Cre-ER-coding region to give pGD19. Then a 2.2 kb insert was released from pGD19 by SmaI-SpeI digestion and ligated to SmaI-NotI digested pKSpdx-1SalI, and a SpeI-NotI digested PCR fragment that contains the SV40 polyadenylation signal to give pGD35. The 8 kb insert was released by SalI-NotI digestion and was used for pronucleus injection. See Author's Map and article for more information. SalI/SmaI digest should give 6.7, 1.5, 4 kb bands. | Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, mouse transgenic; Bacterial Resistance:Ampicillin | 2026-09-05 12:49:31 | 0 | |
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Pdx1 Cre (DM#258) Resource Report Resource Website 1+ mentions |
RRID:Addgene_15021 | Pdx1 promoter | Mus musculus | Ampicillin | PMID:11973276 | To generate the Pdx1-Cre construct, a 5.5 kb SalI-SmaI fragment (blunt-ended) containing the Pdx1 promoter from pKSpdx-1SalI was ligated to the XbaI-digested (blunt-ended) pMC-Cre. The insert was released by SalI and NotI digestion. See Author's Map for more information. | Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, mouse transgenic; Bacterial Resistance:Ampicillin | 2026-09-05 12:49:31 | 1 | |
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pLenti-mIFT88-EGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_149697 | IFT88 | Mus musculus | Ampicillin | Backbone Size:9800; Vector Backbone:pEF1α-IRES-EGFP (IRES-EGFP removed); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-05 12:49:31 | 1 | |||
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pLIA CRX EnR (CC#145) Resource Report Resource Website |
RRID:Addgene_15155 | CRX | Mus musculus | Ampicillin | PMID:9390562 | For the CRX-EnR fusion construct, a 302 bp XmaI fragment (amino acids 7–108) containing the Crx homeobox was fused to the EnR domain. To make the CRX-EnR fusion, the XmaI Crx fragment, an EcoRI-XmaI linker encoding amino acids 2–6 with a NcoI site at the methionine of position 2, an XmaI-ClaI linker, and a ClaI-NotI (partial digestion) 930 bp fragment of the EnR were ligated into a shuttle vector, pSLAX21. The ClaI site inside this construct was designed so that the entire CRX-EnR fusion could be excised as a 1.3 kb ClaI fragment from pSLAX21/CRX-EnR. The ClaI fragment was ligated into pLIA. | Backbone Marker:Cepko Lab; Backbone Size:0; Vector Backbone:pLIA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | amino acids 7–108, containing the CRX homeobox | 2026-09-05 12:49:32 | 0 |
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pBS Rax (CC#5) Resource Report Resource Website |
RRID:Addgene_15138 | Rax | Mus musculus | Ampicillin | PMID:9096350 | Rax cDNA | Backbone Size:3000; Vector Backbone:pBluescript KS+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:49:32 | 0 | |
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pLIA mNIC myc (CC#341) Resource Report Resource Website 1+ mentions |
RRID:Addgene_15131 | Notch1 (C-term) | Mus musculus | Ampicillin | PMID:9006984 | mNIC encodes the cytoplasmic domain of mouse Notch 1 with a myc tag at the N terminus. | Backbone Marker:Cepko Lab; Backbone Size:0; Vector Backbone:pLIA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | Cytoplasmic domain initiating at amino acid 1753. See author's map and article for more information. | 2026-09-05 12:49:32 | 5 |
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pLIA CRX (CC#69) Resource Report Resource Website |
RRID:Addgene_15128 | CRX | Mus musculus | Ampicillin | PMID:9390562 | An EcoRV-HincII Crx cDNA fragment containing the entire Crx coding region was subcloned into the SnaBI site of the pLIA retrovirus vector. | Backbone Marker:Cepko Lab; Backbone Size:6500; Vector Backbone:pLIA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-09-05 12:49:32 | 0 | |
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pBS CRX (CC#4) Resource Report Resource Website |
RRID:Addgene_15127 | CRX | Mus musculus | Ampicillin | PMID:9390562 | CRX cDNA | Backbone Size:3000; Vector Backbone:pBluescript KS+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:49:32 | 0 | |
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nAChR beta2 YFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_15107 | beta2-YFP-M | Mus musculus | Ampicillin | PMID:14684858 | YFP with an upstream HA epitope tag was inserted into PpuMI restriction site of the (M3-M4) loop at residue 381 (beta2-YFP-M). | Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:49:32 | 1 | |
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pLIA-E p27 (CC#620) Resource Report Resource Website |
RRID:Addgene_15191 | p27 | Mus musculus | Ampicillin | PMID:11404411 | Backbone Marker:Cepko Lab; Backbone Size:0; Vector Backbone:pLIA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-09-05 12:49:32 | 0 | ||
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RCAS(A) mWnt1 (CT#165) Resource Report Resource Website |
RRID:Addgene_14017 | Wnt1 | Mus musculus | Ampicillin | Contains an internal Cla I site, from Javier Capdevila. | Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:48:52 | 0 | ||
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pB6-Csrp2-T2A-sfGFP-iCre-ERT2-FNF-DTA Resource Report Resource Website |
RRID:Addgene_140270 | Csrp2 | Mus musculus | Ampicillin | Linker between sfGFP and iCre-ERT2 is a mutated P2A sequence where 2 conserved amino acids are changed to Alanines. This mutated sequence does not skip. The sfGFP fluorescence from the sfGFP-iCre-ERT2 is dim, as described in Nam and Capecchi, 2020 (https://doi.org/10.1186/s13064-020-00139-5) and a Figshare page (https://doi.org/10.6084/m9.figshare.13218437.v35). Thus, the sfGFP fluorescence should be detectable only in cells in which the marker gene expression level is high. Homology arms subcloned by recombineering from a clone of RPCI-24 C57BL/6J mouse genomic DNA library. Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation. Please note there are a few ambiguous bases in Addgene’s NGS result. This is due to repetitive T-A regions. Depositor confirms these bases do not affect plasmid function. | Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:pDTA-4; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin | 2026-09-05 12:48:53 | 0 | ||
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pKLV-XAF1 Resource Report Resource Website |
RRID:Addgene_140290 | XAF1 sgRNA | Mus musculus | Ampicillin | Vector Backbone:pBluescript; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-05 12:48:53 | 0 | |||
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pBS mLmx1 (CT#412) Resource Report Resource Website |
RRID:Addgene_14025 | Lmx1b | Mus musculus | Ampicillin | EcoRI fragment containing full length mouse Lmx-1 cDNA, from Randy Johnson. | Backbone Size:3000; Vector Backbone:pBS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:48:52 | 0 | ||
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RCAS(A) mLef1 (CT#1141) Resource Report Resource Website |
RRID:Addgene_14021 | Lef1 | Mus musculus | Ampicillin | From Gabrielle Kardon. | Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:48:52 | 0 | ||
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pH-nCas9-PPE Resource Report Resource Website |
RRID:Addgene_140447 | nCas9(H840A)-M-MLV | Mus musculus | Kanamycin | PMID:32393904 | Backbone Marker:Qi-Jun Chen (Addgene plasmid # 62203); Vector Backbone:pHUE411; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin | H840A for Cas9; D200N, T306K, W313F, T330P and L603W for M-MLV | 2026-09-05 12:48:54 | 0 | |
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pPIC-mouseKir3.2-mC-S Resource Report Resource Website |
RRID:Addgene_140745 | Kir3.2 | Mus musculus | Bleocin (Zeocin) | PMID:32372643 | Vector Backbone:pPICZ; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin) | 2026-09-05 12:48:56 | 0 | ||
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pEYFP-C1-p85beta Resource Report Resource Website 1+ mentions |
RRID:Addgene_1408 | PI3K regulatory subunit p85beta | Mus musculus | Kanamycin | Cantley lab recommends growth at room temperature. | Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-09-05 12:48:56 | 4 |
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