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Species: Danio rerio
Genetic Insert: 3x3 Stop sequence
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-mRFP, Oline tools and cloning help avalible at
Proper citation: RRID:Addgene_117775 Copy
Species: Danio rerio
Genetic Insert: 3x3 Stop sequence
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-mRFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117772 Copy
Species: Danio rerio
Genetic Insert: gal4/VP16
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-mRFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117778 Copy
Species: Danio rerio
Genetic Insert: tp53 gRNA
Vector Backbone Description: Vector Backbone:MiniCoopR; Vector Types:CRISPR, Tol2; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30385465
Proper citation: RRID:Addgene_118841 Copy
Species: Danio rerio
Genetic Insert: spred1 gRNA
Vector Backbone Description: Vector Backbone:MiniCoopR; Vector Types:CRISPR, Tol2; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30385465
Proper citation: RRID:Addgene_118843 Copy
Species: Danio rerio
Genetic Insert: cdkn2a gRNA
Vector Backbone Description: Vector Backbone:MiniCoopR; Vector Types:CRISPR, Tol2; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30385465
Proper citation: RRID:Addgene_118842 Copy
Species: Danio rerio
Genetic Insert: ptena and ptenb
Vector Backbone Description: Vector Backbone:MiniCoopR; Vector Types:CRISPR, Tol2; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30385465
Proper citation: RRID:Addgene_118845 Copy
Species: Danio rerio
Genetic Insert: mitfa: EGFP
Vector Backbone Description: Vector Backbone:MiniCoopR; Vector Types:Zebrafish expression, Tol6; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30385465
Proper citation: RRID:Addgene_118850 Copy
Species: Danio rerio
Genetic Insert: U6a:sgRNA
Vector Backbone Description: Backbone Size:3565; Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37367831
Comments: Please visit https://www.biorxiv.org/content/10.1101/450684v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_119069 Copy
Species: Danio rerio
Genetic Insert: U6a:2xCom-sgRNA
Vector Backbone Description: Backbone Size:3565; Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37367831
Comments: Please visit https://www.biorxiv.org/content/10.1101/450684v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_119077 Copy
Species: Danio rerio
Genetic Insert: U6a:scrambled1-sgRNA
Vector Backbone Description: Backbone Size:3577; Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37367831
Comments: Contains scrambled spacer sequence AGCGCCTACGCGCATGGCCT.
Please visit https://www.biorxiv.org/content/10.1101/450684v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_119070 Copy
Species: Danio rerio
Genetic Insert: U6a:scrambled2-sgRNA
Vector Backbone Description: Backbone Size:3577; Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37367831
Comments: Contains scrambled spacer sequence GGCCACAGGCGATTATCGCC.
Please visit https://www.biorxiv.org/content/10.1101/450684v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_119071 Copy
Species: Danio rerio
Genetic Insert: Rab8B
Vector Backbone Description: Backbone Size:2500; Vector Backbone:pDONR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17574030
Comments: This construct was generated by gene synthesis and is based on a reference sequence from 2007. Amino acids NE 178-179 are missing compared to current NCBI sequences.
Proper citation: RRID:Addgene_24908 Copy
Species: Danio rerio
Genetic Insert: fishRab8B
Vector Backbone Description: Backbone Size:2500; Vector Backbone:pDONR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17574030
Comments: This construct was generated by gene synthesis and is based on a reference sequence from 2007. Amino acids NE 178-179 are missing compared to current NCBI sequences.
Proper citation: RRID:Addgene_24907 Copy
Species: Danio rerio
Genetic Insert: fli1a intron 1
Vector Backbone Description: Backbone Size:2858; Vector Backbone:p5E; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23462469
Comments: "p5E" refers to 5' entry clones flanked by L4-R1
There are a few mismatches between depositor's sequence and Addgene's sequence. The changes should not affect plasmid function.
Proper citation: RRID:Addgene_26027 Copy
Species: Danio rerio
Genetic Insert: miR-126
Vector Backbone Description: Backbone Size:0; Vector Backbone:pME; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20364122
Comments: The insert includes genomic sequence flanking the mature miR-126 sequence on chromosome 8 (miRBase accession number MI0001979, Zv8).
There is a C->T mutation in the miR126. The mutation is probably due to a SNP and should not affect plasmid function.
Proper citation: RRID:Addgene_26033 Copy
Species: Danio rerio
Genetic Insert: VSP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_23959 Copy
Species: Danio rerio
Genetic Insert: VSP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_23960 Copy
Species: Danio rerio
Genetic Insert: H2B
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Worm Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19706417
Comments: This plasmid was constructed using 1.5 kb of the hsp70l promoter. The floxed mCherry-STOP and H2b_GFP fragments were PCR amplified individually and sequentially cloned downstream of the hsp70l promoter. The mCherry-STOP cassette serves as a heat-shock induction marker prevents read-through translation of the H2B-GFP fusion protein, which is only expressed in cells that have undergone a Cre mediated excision event. cryaa-Cerulean was inserted downstream of HSB-GFP in the reverse orientation.
*H2B has D26G and V119I mutations. According to the depositor, GFP expression from this construct was always nuclear so there is no reason to suspect that the mutations affect the function (nuclear localization) of the H2B fragment.
Proper citation: RRID:Addgene_24334 Copy
Species: Danio rerio
Genetic Insert: Cavin4b
Vector Backbone Description: Vector Backbone:SITS-ccdb-EGFP (Addgene 118752); Vector Types:Leishmania cell free expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176019 Copy
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