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http://www.addgene.org/116062

Species: Synthetic
Genetic Insert: TEVP
Vector Backbone Description: Vector Backbone:CMV-TO-FlpIn; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30237357

Proper citation: RRID:Addgene_116062 Copy   

•   Source: Addgene

http://www.addgene.org/116157

Species: Homo sapiens
Genetic Insert: BRAF
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785

Proper citation: RRID:Addgene_116157 Copy   

•   Source: Addgene

http://www.addgene.org/1161

Species: Saccharomyces cerevisiae
Genetic Insert: HSP104
Vector Backbone Description: Backbone Size:5000; Vector Backbone:TMBS (CEN TRP1 MET3 promoter); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14978213

Proper citation: RRID:Addgene_1161 Copy   

•   Source: Addgene

http://www.addgene.org/11619

Vector Backbone Description: Backbone Marker:N/A; Backbone Size:8500; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16550170
Comments: pLB is a modification of pLL3.7. Two genetic elements known to prevent epigenetic silencing were added. A fragment of one antirepressor element (#40) was cloned upstream of the mouse U6 promoter and a scaffold-attached region (SAR) was cloned downstream of GFP. Please see author's map for more detailed information. Note: A single base pair deletion at position 11 of the mouse U6 promoter in this plasmid does not impair the efficacy of this reagent. There is also a base pair insertion upstream of the promoter. The depositing lab has no indication that it functionally impairs pLB.

Proper citation: RRID:Addgene_11619 Copy   

•   Source: Addgene

http://www.addgene.org/116276

Species: Homo sapiens
Genetic Insert: EGFR
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785

Proper citation: RRID:Addgene_116276 Copy   

•   Source: Addgene

http://www.addgene.org/11629

Species: Homo sapiens
Genetic Insert: merlin
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4950; Vector Backbone:pGEX2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10567424
Comments: The merlin insert in this plasmid contains A190V and G302E mutations compared to the closest match human Merlin reference sequence (NP_000259.1). The depositing laboratory states that these mutations should not affect merlin function, but cautions that it may be necessary to test merlin function if performing structure function experiments.

Proper citation: RRID:Addgene_11629 Copy   

•   Source: Addgene

http://www.addgene.org/116222

Species: Homo sapiens
Genetic Insert: CDKN2A
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785

Proper citation: RRID:Addgene_116222 Copy   

•   Source: Addgene

http://www.addgene.org/116204

Species: Homo sapiens
Genetic Insert: BRAF
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785

Proper citation: RRID:Addgene_116204 Copy   

•   Source: Addgene

http://www.addgene.org/11639

Species: Homo sapiens
Genetic Insert: moesin
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4970; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10567424

Proper citation: RRID:Addgene_11639 Copy   

•   Source: Addgene

http://www.addgene.org/11636

Species: Homo sapiens
Genetic Insert: NHE-RF
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4970; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10567424

Proper citation: RRID:Addgene_11636 Copy   

•   Source: Addgene

http://www.addgene.org/11635

Species: Homo sapiens
Genetic Insert: NHE-RF
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3/5'F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10567424

Proper citation: RRID:Addgene_11635 Copy   

•   Source: Addgene

http://www.addgene.org/11637

Species: Homo sapiens
Genetic Insert: moesin
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4970; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10567424

Proper citation: RRID:Addgene_11637 Copy   

•   Source: Addgene

http://www.addgene.org/116372

Species: Homo sapiens
Genetic Insert: ESR1
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785

Proper citation: RRID:Addgene_116372 Copy   

•   Source: Addgene

http://www.addgene.org/116373

Species: Homo sapiens
Genetic Insert: ESR1
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785

Proper citation: RRID:Addgene_116373 Copy   

•   Source: Addgene

http://www.addgene.org/116374

Species: Homo sapiens
Genetic Insert: ESR1
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785

Proper citation: RRID:Addgene_116374 Copy   

•   Source: Addgene

http://www.addgene.org/11643

Genetic Insert: CAG promoters, GFP, tTR-KRAB, Tet-on
Vector Backbone Description: Backbone Size:12877; Vector Backbone:None; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16432520
Comments: Transgenes can be expressed from any RNA Pol II promoter as part of bicistronic unit comprising the KRAB-based repressor; tetO sequences are inserted into the vector LTR. Tet-on and Tet-off versions rely on repressors that bind in the absence or the presence of doxycycline, respectively. Addition of Pol III promoter-small hairpin RNA cassette allows for drug-controllable RNA interference (Tet-on shRNA). Cloning shRNA into pLVET, pLVCT, and pLVPT vectors: first clone shRNA into pLVTHM downstream of the tetO-H1 region. Then cut pLVTHM containing your shRNA with MscI-FspI and clone the insert containing the 3'LTR to target plasmid opened with MscI-FspI. FspI cuts into AmpR. This means for inverted clones AmpR will not be restored; after selection you will be left with clones with the shRNA cassette and functional AmpR. pLVTHM and packaging plasmids for this system are also available at Addgene http://www.addgene.org/rnaitools Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab website http://tronolab.epfl.ch to see frequently asked questions on cloning strategies and packaging.

Proper citation: RRID:Addgene_11643 Copy   

•   Source: Addgene

http://www.addgene.org/11645

Genetic Insert: hUbiquitin C, GFP, tTR-KRAB, no tetO
Vector Backbone Description: Backbone Size:11558; Vector Backbone:None; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16432520
Comments: pLVTHM and packaging plasmid for this system are also available at Addgene http://www.addgene.org/rnaitools Please visit Trono lab website http://tronolab.epfl.ch to see frequently asked questions on cloning strategies and packaging.

Proper citation: RRID:Addgene_11645 Copy   

•   Source: Addgene

http://www.addgene.org/11644

Genetic Insert: hEF-1alpha, GFP, tTR-KRAB, Tet-on
Vector Backbone Description: Backbone Size:12386; Vector Backbone:None; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16432520
Comments: Transgenes can be expressed from any RNA Pol II promoter as part of bicistronic unit comprising the KRAB-based repressor; tetO sequences are inserted into the vector LTR. Tet-on and Tet-off versions rely on repressors that bind in the absence or the presence of doxycycline, respectively. Addition of Pol III promoter-small hairpin RNA cassette allows for drug-controllable RNA interference (Tet-on shRNA). Cloning shRNA into pLVET, pLVCT, and pLVPT vectors: first clone shRNA into pLVTHM downstream of the tetO-H1 region. Then cut pLVTHM containing your shRNA with MscI-FspI and clone the insert containing the 3'LTR to target plasmid opened with MscI-FspI. FspI cuts into AmpR. This means for inverted clones AmpR will not be restored; after selection you will be left with clones with the shRNA cassette and functional AmpR. pLVTHM and packaging plasmid for this system are also available at Addgene http://www.addgene.org/rnaitools Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit Trono lab website http://tronolab.epfl.ch to see frequently asked questions on cloning strategies and packaging.

Proper citation: RRID:Addgene_11644 Copy   

•   Source: Addgene

http://www.addgene.org/116428

Species: Homo sapiens
Genetic Insert: MAP2K1
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785

Proper citation: RRID:Addgene_116428 Copy   

•   Source: Addgene

http://www.addgene.org/116424

Species: Homo sapiens
Genetic Insert: KRAS
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785

Proper citation: RRID:Addgene_116424 Copy   

•   Source: Addgene