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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pBAC-lacZ Resource Report Resource Website 1+ mentions |
RRID:Addgene_13422 | Chloramphenicol | PMID:17406455 | The complete assembled sequence for this plasmid may not be accurate. Addgene suggests sequence verifying the construct before starting your experiments. Bacterial cell-based two-hybrid (B2H) reporter vector. Use 12.5 ug/ml chloramphenicol for growth. The pBAC-lacZ plasmid is a mini-F' that can be replicated in standard E. coli strains, but because it is maintained as a single copy episome, it gives low DNA yields. pBAC-lacZ also contains a second, higher copy number origin of replication (oriV) that is only active in the presence of a trans-acting factor encoded by the trfA gene. Transformax EPI300 cells express trfA from an inducible promoter that we have found is inducible with arabinose. When the trfA gene is induced in Transformax EPI300 cells, the copy number of pBAC-lacZ plasmid is increased in the cells and reasonable yields of plasmid can be obtained using a standard miniprep procedure. Further notes on propagating this plasmid: grow an overnight culture (without arabinose) and then the next morning subculture 1:10 into medium that has a final concentration of arabinose of 1 mM. You then grow this culture for 5 to 6 hours at 37 C and then harvest the DNA. | Backbone Size:11151; Vector Backbone:pBAC-lacZ; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol | 2026-09-05 12:47:57 | 5 | |||
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U6 pShuttle Resource Report Resource Website 1+ mentions |
RRID:Addgene_13428 | Kanamycin | pShuttle is used to make adenovirus in the AdEasy system. The human U6 promoter allows for siRNA expression. | Backbone Size:0; Vector Backbone:pShuttle; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin | 2026-09-05 12:47:58 | 2 | ||||
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pcDNA6-GK5_v2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_134280 | Gk5 | Mus musculus | Ampicillin | PMID:28607088 | Backbone Size:5000; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | mouse Gk5 isoform 2; 534 amino acids | 2026-09-05 12:47:58 | 1 | |
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pcDNA3.1-P25C-GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_1343 | P25 | Homo sapiens | Ampicillin | Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:47:58 | 2 | |||
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HP138-neo Resource Report Resource Website 1+ mentions |
RRID:Addgene_134247 | Contains Cas9 | Other | Ampicillin | PMID:26698661 | Vector Backbone:unknown; Vector Types:CRISPR; Bacterial Resistance:Ampicillin | 2026-09-05 12:47:57 | 4 | ||
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pFA6a-A1:natMX4 Resource Report Resource Website 1+ mentions |
RRID:Addgene_134428 | A1 | Synthetic | Ampicillin | PMID:32401365 | Vector Backbone:pFA6a-natMX4; Vector Types:PCR-based tagging in yeast; Bacterial Resistance:Ampicillin | 2026-09-05 12:47:59 | 1 | ||
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px458_Conc2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_134449 | Ampicillin | PMID:32150173 | The sgRNAs can get introduced by golden gate cloning like described in Andersson-Rolf A et al.: Simultaneous paralogue knockout using a CRISPR-concatemer in mouse small intestinal organoids. Dev Biol. 2016. doi: 10.1016/j.ydbio.2016.10.016. For more information see protocol. | Backbone Marker:Feng Zhang; Vector Backbone:pSpCas9(BB)-2A-GFP (PX458); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-09-05 12:47:59 | 1 | |||
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pcDNA3-dnCDK5 Resource Report Resource Website 1+ mentions |
RRID:Addgene_1344 | dnCDK5 | Homo sapiens | Ampicillin | Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | D144N | 2026-09-05 12:47:58 | 3 | ||
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mARG1 cassette 2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_134344 | mARG 1 cassete 2 | Bacillus megaterium | Ampicillin | PMID:31604277 | Vector Backbone:pAF PB; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-09-05 12:47:58 | 1 | ||
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mARG1 cassette 3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_134345 | mARG 1 cassete 3 | Bacillus megaterium | Ampicillin | PMID:31604277 | Vector Backbone:pAF PB; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-09-05 12:47:58 | 1 | ||
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pYPQ131C-RZ-Lb Resource Report Resource Website 1+ mentions |
RRID:Addgene_134347 | Tetracycline | PMID:31730252 | Vector Backbone:pYPQ131C; Vector Types:CRISPR; Bacterial Resistance:Tetracycline | 2026-09-05 12:47:58 | 1 | ||||
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pLITMUS*-PBBa_J23106-geneIII Resource Report Resource Website 1+ mentions |
RRID:Addgene_134352 | Gene III | Ampicillin | PMID:32577520 | Vector Backbone:pLITMUS*; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:47:58 | 1 | |||
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pCS2+ MT Beta Catenin (Xenopus) Resource Report Resource Website 1+ mentions |
RRID:Addgene_13435 | Beta Catenin | Xenopus laevis | Ampicillin | PMID:11524435 | Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:47:58 | 2 | ||
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pBbAW4k-loxP-TT-loxP-mRFP1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_134405 | W4-loxP-TT-loxP-mRFP1 | Kanamycin | PMID:31961670 | Please visit https://www.biorxiv.org/content/10.1101/786533v1 for bioRxiv preprint. Please note: This plasmid exists as a multimer. This is not expected to impact the end function of the plasmid. Please refer to the Addgene Blog post on multimers for more information: https://blog.addgene.org/plasmids-101-dimers-and-multimers | Backbone Size:2432; Vector Backbone:pBbA-k; Vector Types:Bacterial Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-09-05 12:47:59 | 1 | ||
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PGKneotpAlox2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_13444 | PGKneotpAlox2 | Ampicillin | PMID:9916792 | This vector contains: phosphoglycerate kinase I (PGK) promoter, neomycin resistance gene (neo), PGK polyA site, and 3x SV40 polyA site (tpA). The PGKneotpA is flanked by loxP sites. | Backbone Size:2900; Vector Backbone:na; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin | 2026-09-05 12:47:59 | 5 | ||
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PGKneobpA Resource Report Resource Website 1+ mentions |
RRID:Addgene_13442 | PGKneobpA | Ampicillin | PMID:2016762 | This vector contains: phosphoglycerate kinase I (PGK) promoter, neomycin resistance gene (neo), bovine growth hormone polyadenylation site (bpA). | Backbone Size:2900; Vector Backbone:na; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:47:59 | 7 | ||
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PGKneolox2DTA Resource Report Resource Website 1+ mentions |
RRID:Addgene_13443 | PGKneolox2DTA | Ampicillin | PMID:9226440 | This vector contains PGKneobpA flanked by loxP sites and PGKdtabpA. | Backbone Size:2900; Vector Backbone:na; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin | 2026-09-05 12:47:59 | 5 | ||
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pGEX-KG-D4H*-mCherry Resource Report Resource Website 1+ mentions |
RRID:Addgene_134604 | D4H* | Synthetic | Ampicillin | PMID:31548609 | https://benchling.com/s/seq-qYjuRHB1UTwPC8BVFbvK | Backbone Size:5006; Vector Backbone:pGEX-KG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Y415A and A463W | 2026-09-05 12:48:00 | 7 |
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pEGFP-N1-Flag-humanRBM47 Resource Report Resource Website 1+ mentions |
RRID:Addgene_134607 | Flag-humanRBM47 | Homo sapiens | Kanamycin | Backbone Size:3960; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-09-05 12:48:00 | 1 | |||
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px459V2.0_Conc2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_134451 | Ampicillin | PMID:32150173 | The sgRNAs can get introduced by golden gate cloning like described in Andersson-Rolf A et al.: Simultaneous paralogue knockout using a CRISPR-concatemer in mouse small intestinal organoids. Dev Biol. 2016. doi: 10.1016/j.ydbio.2016.10.016. For more information see protocol. | Backbone Marker:Feng Zhang; Vector Backbone:pSpCas9(BB)-2A-Puro (PX459) V2.0; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-09-05 12:47:59 | 4 |
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