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Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2G_2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149617 Copy
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2G_2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149618 Copy
Species: Synthetic
Genetic Insert: dCas9, lacI, sgRNAftsI
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S(RBSmut); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149590 Copy
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149581 Copy
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S(-10TC); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149582 Copy
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9B(-10TC); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Blasticidin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149586 Copy
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9H(-10TC); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149587 Copy
Species: Synthetic
Genetic Insert: dCas9, lacI, sgRNAflaB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S(RBSmut); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257311
Comments: Addgene QC identified an IS element present in the backbone sequence. The depositing lab does not believe this impacts the functional use of the plasmid but requesting scientists should validate expected behavior prior to experimentation.
Proper citation: RRID:Addgene_149588 Copy
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2G_2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149621 Copy
Species: Synthetic
Genetic Insert: dCas9, lacI, sgRNAflaB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S(-10TC); Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149589 Copy
Species: Synthetic
Genetic Insert: sgRNAflaB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2G_2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149627 Copy
Species: Synthetic
Genetic Insert: sgRNAflaB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2G_2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149628 Copy
Species: Synthetic
Genetic Insert: sgRNAflaB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2G_2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149629 Copy
Species: Homo sapiens
Genetic Insert: human frataxin cDNA
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pBluescript II; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_14988 Copy
Species: Homo sapiens
Genetic Insert: human frataxin cDNA
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pBluescript II; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_14989 Copy
Species: Homo sapiens
Genetic Insert: human frataxin cDNA
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pBluescript II; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11929854
Proper citation: RRID:Addgene_14987 Copy
Vector Backbone Description: Backbone Size:7562; Vector Backbone:pBigT; Vector Types:Cre/Lox, for making transgenic constructs; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14657333
Comments: Modification of plasmid pBigT described in Srinivas et al. BMC Dev Biol (2001) vol 1, p.4. For use with pRosa26PA. See BMC Dev Biol article for more information.
Modified to contain IRES-EGFP downstream of cloning site. See Author's Map for more information.
Proper citation: RRID:Addgene_15037 Copy
Species: Xenopus laevis
Genetic Insert: beta-globin 5' and 3' UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pSP64; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:6207484
Comments: Vector with Xenopus 5' and 3' untranslated beta-globin sequences to enhance translation of synthetic mRNAs. Cloning site for mRNA is BglII. See author's map for more information.
Proper citation: RRID:Addgene_15030 Copy
Species: Homo sapiens
Genetic Insert: Brca1
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10550055
Comments: pBABEpuro HABrca1 was made by digesting pCDNA3beta HABrca1 (R. Scully, et al., Cell 88, 265, 1997) with SalI and XhoI and inserting it into the SalI site of pBABEpuro.
The BRCA1 insert in this plasmid contains the following common polymorphisms when compared to GenBank reference sequence NP_009225.1: P871L, E1038G, K1182R, and S1613G. These SNPs were likely present in the original cDNA isolate.
Proper citation: RRID:Addgene_14999 Copy
Species: Mus musculus
Genetic Insert: Mer
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:5300; Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15673687
Comments: The full-length mouse Mer (994 amino acids) was cloned from a 16-day-old mouse embryo cDNA library. Addgene's quality control found the closest match to the insert sequence is mouse Mer (AA17-994) Sequence ID: NP_032613.1, with mutations I516V and Q860R. The plasmid should function as described in the associated article listed on the plasmid page above.
Proper citation: RRID:Addgene_14998 Copy
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