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Species: Synthetic
Genetic Insert: human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP
Vector Backbone Description: Backbone Marker:Kleinstiver Lab (Addgene ID 139987); Vector Backbone:RTW3027; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Proper citation: RRID:Addgene_139991 Copy
Species: Synthetic
Genetic Insert: human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
Vector Backbone Description: Backbone Marker:Ketih Joung; Vector Backbone:pRZ33 ; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Proper citation: RRID:Addgene_139999 Copy
Species: Synthetic
Genetic Insert: human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
Vector Backbone Description: Backbone Marker:Kleinstiver Lab (Addgene ID 139987); Vector Backbone:RTW3027; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Proper citation: RRID:Addgene_139997 Copy
Species: Synthetic
Genetic Insert: human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
Vector Backbone Description: Backbone Marker:David Liu (Addgene Plasmid 112101); Vector Backbone:pCMV_ABEmax_P2A_GFP; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Comments: The depositing laboratory has released the equivalent ABE8e versions of this enzyme, which they have found to show much higher editing efficiency. See https://www.addgene.org/browse/article/28228617/ for the plasmids.
Proper citation: RRID:Addgene_140002 Copy
Species: Synthetic
Genetic Insert: human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
Vector Backbone Description: Backbone Marker:David Liu (Addgene Plasmid 112101); Vector Backbone:pCMV_ABEmax_P2A_GFP; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Comments: The depositing laboratory has released the equivalent ABE8e versions of this enzyme, which they have found to show much higher editing efficiency. See https://www.addgene.org/browse/article/28228617/ for the plasmids.
Proper citation: RRID:Addgene_140003 Copy
Species: Synthetic
Genetic Insert: human codon optimized CBE4max xCas9(3.7) with P2A-EGFP
Vector Backbone Description: Backbone Marker:Ketih Joung; Vector Backbone:pRZ33; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Proper citation: RRID:Addgene_140000 Copy
Species: Mus musculus
Genetic Insert: Bmp4
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRIAS; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: From Devyn Smith.
Proper citation: RRID:Addgene_14001 Copy
Species: Drosophila melanogaster
Genetic Insert: RhoA
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11239392
Proper citation: RRID:Addgene_14002 Copy
Species: Synthetic
Genetic Insert: pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
Vector Backbone Description: Backbone Marker:Joung Lab (DR274); Vector Backbone:DR274 (Addgene Plasmid #42250); Vector Types:Bacterial Expression, In vitro transcription (mRNA synthesis); Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_140082 Copy
Species: Aequorea victoria
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:pAS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30260624
Proper citation: RRID:Addgene_140015 Copy
Species: Methanomethylophilus alvus Mx1201
Genetic Insert: Mx1201 PylRS
Vector Backbone Description: Vector Backbone:pAS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30260624
Comments: Please note there is a T72A point mutation in Neo/KanR. Depositor confirms this does not affect function.
Proper citation: RRID:Addgene_140011 Copy
Species: Escherichia coli
Genetic Insert: EcoTyrRS
Vector Backbone Description: Vector Backbone:pAS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37935196
Proper citation: RRID:Addgene_140018 Copy
Species: Synthetic
Genetic Insert: Salsa6f
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3923; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29239725
Comments: The fusion protein in this plasmid is the fluorescent genetically encoded calcium indicator Salsa6f, which is a fusion of the dimeric red fluorescent protein tdTomato, the epitope tag V5, and the green fluorescent genetically encoded calcium indicator GCaMP6f, in order from N- to C-termini. The presence of tdTomato enables visualization of transfected cells in the absence of Ca2+ signaling and concentration-independent, ratiometric readout of Ca2+ signals. Salsa6f has been introduced into transgenic mice, where its expression is under Cre recombinase control (see PMID: 29239725).
Proper citation: RRID:Addgene_140188 Copy
Species: Other
Genetic Insert: FlaviA-GFP
Vector Backbone Description: Backbone Marker:Invitrogen modified by Ie-Ming Shih; Backbone Size:7057; Vector Backbone:pLenti-puro (Addgene Plasmid #39481); Vector Types:Mammalian Expression, Lentiviral, Low expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31919100
Comments: The FlaviA-GFP reporter is not suitable for transient transfection as the reporter has a basal background that makes it sensitive to the levels of expression: If the expression is too high the background will mask the signal produced by the reporter - If the expression is too low you won’t detect enough signal over the background. Our recommendation is to use the pLenti-FlaviA-GFP-puro construct to pack lentiviral particles with the psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) plasmids, generate stable cell lines and sort cell subpopulations with different levels of the reporter's basal background before testing them. The best reporter cells will be those with the highest signal-to-noise ratio upon flavivirus infection.
Proper citation: RRID:Addgene_140088 Copy
Species: Mus musculus
Genetic Insert: Tcf4B
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14667415
Proper citation: RRID:Addgene_14019 Copy
Species: Synthetic
Genetic Insert: Citroff1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32875085
Proper citation: RRID:Addgene_134301 Copy
Genetic Insert: Citron1
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32875085
Proper citation: RRID:Addgene_134303 Copy
Species: Synthetic
Genetic Insert: Citron1
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32875085
Proper citation: RRID:Addgene_134305 Copy
Vector Backbone Description: Backbone Size:5725; Vector Backbone:pST1374; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17406455
Comments: Human zinc finger nuclease cloning vector. Zinc fingers are cloned in between unique XbaI and BamHI sites in front of the FokI nuclease domain.
Proper citation: RRID:Addgene_13426 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pShuttle; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Comments: pShuttle is used to make adenovirus in the AdEasy system. The CAG promoter was inserted for stronger expression and a MCS compatible with pBS.
See author's map for more information.
Proper citation: RRID:Addgene_13427 Copy
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