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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pCMV-T7-SpCas9-VRER-P2A-EGFP (RTW3160) Resource Report Resource Website 1+ mentions |
RRID:Addgene_139991 | human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP | Synthetic | Ampicillin | PMID:32217751 | Backbone Marker:Kleinstiver Lab (Addgene ID 139987); Vector Backbone:RTW3027; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin | VRER=D1135V/G1218R/R1335E/T1337R | 2026-09-05 12:48:50 | 1 | |
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pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133) Resource Report Resource Website 10+ mentions |
RRID:Addgene_139999 | human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP | Synthetic | Ampicillin | PMID:32217751 | Backbone Marker:Ketih Joung; Vector Backbone:pRZ33 ; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R | 2026-09-05 12:48:50 | 13 | |
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pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008) Resource Report Resource Website 1+ mentions |
RRID:Addgene_139997 | human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP | Synthetic | Ampicillin | PMID:32217751 | Backbone Marker:Kleinstiver Lab (Addgene ID 139987); Vector Backbone:RTW3027; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin | SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R; HF1=N497A/R661A/Q695A/Q926A | 2026-09-05 12:48:50 | 1 | |
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pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562) Resource Report Resource Website 1+ mentions |
RRID:Addgene_140002 | human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP | Synthetic | Ampicillin | PMID:32217751 | The depositing laboratory has released the equivalent ABE8e versions of this enzyme, which they have found to show much higher editing efficiency. See https://www.addgene.org/browse/article/28228617/ for the plasmids. | Backbone Marker:David Liu (Addgene Plasmid 112101); Vector Backbone:pCMV_ABEmax_P2A_GFP; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin | nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R | 2026-09-05 12:48:50 | 9 |
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pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) Resource Report Resource Website 10+ mentions |
RRID:Addgene_140003 | human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP | Synthetic | Ampicillin | PMID:32217751 | The depositing laboratory has released the equivalent ABE8e versions of this enzyme, which they have found to show much higher editing efficiency. See https://www.addgene.org/browse/article/28228617/ for the plasmids. | Backbone Marker:David Liu (Addgene Plasmid 112101); Vector Backbone:pCMV_ABEmax_P2A_GFP; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin | nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R | 2026-09-05 12:48:50 | 11 |
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pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637) Resource Report Resource Website 1+ mentions |
RRID:Addgene_140000 | human codon optimized CBE4max xCas9(3.7) with P2A-EGFP | Synthetic | Ampicillin | PMID:32217751 | Backbone Marker:Ketih Joung; Vector Backbone:pRZ33; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/E543D/M694I/E1219V | 2026-09-05 12:48:50 | 1 | |
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pRIAS mBmp4 (CT#578) Resource Report Resource Website 1+ mentions |
RRID:Addgene_14001 | Bmp4 | Mus musculus | Ampicillin | From Devyn Smith. | Backbone Size:0; Vector Backbone:pRIAS; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-09-05 12:48:50 | 2 | ||
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RCAS(A) dRhoA N19 (CT#847) Resource Report Resource Website 1+ mentions |
RRID:Addgene_14002 | RhoA | Drosophila melanogaster | Ampicillin | PMID:11239392 | Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin | T19N | 2026-09-05 12:48:50 | 1 | |
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pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485) Resource Report Resource Website 1+ mentions |
RRID:Addgene_140082 | pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485) | Synthetic | Kanamycin | Backbone Marker:Joung Lab (DR274); Vector Backbone:DR274 (Addgene Plasmid #42250); Vector Types:Bacterial Expression, In vitro transcription (mRNA synthesis); Bacterial Resistance:Kanamycin | 2026-09-05 12:48:51 | 1 | |||
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pAS_4xMma PylT_GFP 150TAG 6xHis Resource Report Resource Website 1+ mentions |
RRID:Addgene_140015 | sfGFP | Aequorea victoria | Ampicillin | PMID:30260624 | Vector Backbone:pAS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 150TAG | 2026-09-05 12:48:50 | 1 | |
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pAS_4xMx PylT_FLAG-Mx PylRS Resource Report Resource Website 1+ mentions |
RRID:Addgene_140011 | Mx1201 PylRS | Methanomethylophilus alvus Mx1201 | Ampicillin | PMID:30260624 | Please note there is a T72A point mutation in Neo/KanR. Depositor confirms this does not affect function. | Vector Backbone:pAS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:48:50 | 1 | |
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pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG Resource Report Resource Website 1+ mentions |
RRID:Addgene_140018 | EcoTyrRS | Escherichia coli | Ampicillin | PMID:37935196 | Vector Backbone:pAS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Y37L, D182S, F183M, L186A | 2026-09-05 12:48:50 | 2 | |
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Salsa6f Resource Report Resource Website 1+ mentions |
RRID:Addgene_140188 | Salsa6f | Synthetic | Kanamycin | PMID:29239725 | The fusion protein in this plasmid is the fluorescent genetically encoded calcium indicator Salsa6f, which is a fusion of the dimeric red fluorescent protein tdTomato, the epitope tag V5, and the green fluorescent genetically encoded calcium indicator GCaMP6f, in order from N- to C-termini. The presence of tdTomato enables visualization of transfected cells in the absence of Ca2+ signaling and concentration-independent, ratiometric readout of Ca2+ signals. Salsa6f has been introduced into transgenic mice, where its expression is under Cre recombinase control (see PMID: 29239725). | Backbone Marker:Clontech; Backbone Size:3923; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-09-05 12:48:52 | 1 | |
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pLenti-FlaviA-GFP-puro Resource Report Resource Website 1+ mentions |
RRID:Addgene_140088 | FlaviA-GFP | Other | Ampicillin | PMID:31919100 | The FlaviA-GFP reporter is not suitable for transient transfection as the reporter has a basal background that makes it sensitive to the levels of expression: If the expression is too high the background will mask the signal produced by the reporter - If the expression is too low you won’t detect enough signal over the background. Our recommendation is to use the pLenti-FlaviA-GFP-puro construct to pack lentiviral particles with the psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) plasmids, generate stable cell lines and sort cell subpopulations with different levels of the reporter's basal background before testing them. The best reporter cells will be those with the highest signal-to-noise ratio upon flavivirus infection. | Backbone Marker:Invitrogen modified by Ie-Ming Shih; Backbone Size:7057; Vector Backbone:pLenti-puro (Addgene Plasmid #39481); Vector Types:Mammalian Expression, Lentiviral, Low expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:48:51 | 1 | |
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RCAS(A) mTcf4B (CT#1126) Resource Report Resource Website 1+ mentions |
RRID:Addgene_14019 | Tcf4B | Mus musculus | Ampicillin | PMID:14667415 | Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:48:52 | 2 | ||
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pBAD-Citroff1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_134301 | Citroff1 | Synthetic | Ampicillin | PMID:32875085 | Backbone Size:4000; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:47:58 | 1 | ||
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CMV-Citron1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_134303 | Citron1 | Ampicillin | PMID:32875085 | Backbone Size:5300; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:47:58 | 3 | |||
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CMV-mito-Citron1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_134305 | Citron1 | Synthetic | Ampicillin | PMID:32875085 | Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:47:58 | 3 | ||
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pST1374 Resource Report Resource Website 1+ mentions |
RRID:Addgene_13426 | Ampicillin | PMID:17406455 | Human zinc finger nuclease cloning vector. Zinc fingers are cloned in between unique XbaI and BamHI sites in front of the FokI nuclease domain. | Backbone Size:5725; Vector Backbone:pST1374; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:47:57 | 9 | |||
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CAG pShuttle Resource Report Resource Website 1+ mentions |
RRID:Addgene_13427 | Kanamycin | pShuttle is used to make adenovirus in the AdEasy system. The CAG promoter was inserted for stronger expression and a MCS compatible with pBS. See author's map for more information. | Backbone Size:0; Vector Backbone:pShuttle; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin | 2026-09-05 12:47:57 | 2 |
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