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Genetic Insert: myrTagRFP-T-P2A-post-eGRASP
Vector Backbone Description: Backbone Size:5293; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29700265
Proper citation: RRID:Addgene_111581 Copy
Genetic Insert: EGFP from Aequorea victoria
Vector Backbone Description: Backbone Marker:Available at Addgene (#11160); Backbone Size:4779; Vector Backbone:pCAGEN; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14603031
Comments: Please note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this region of the plasmid.
Proper citation: RRID:Addgene_11150 Copy
Genetic Insert: yellow pre-eGRASP(p30)
Vector Backbone Description: Backbone Size:4154; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29700265
Proper citation: RRID:Addgene_111587 Copy
Genetic Insert: CMV promoter-EGFP
Vector Backbone Description: Backbone Size:3084; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14603031
Proper citation: RRID:Addgene_11153 Copy
Genetic Insert: myrmScarlet-I-P2A-post-eGRASP
Vector Backbone Description: Backbone Size:4377; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29700265
Proper citation: RRID:Addgene_111584 Copy
Genetic Insert: HcRed1 from Heteractis crispa
Vector Backbone Description: Backbone Marker:Available at Addgene (#11160); Backbone Size:4779; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14603031
Comments: CAG promoter (chicken beta-actin promoter with CMV enhancer) is more efficient than CMV promoter, see "Efficient selection for high-expression transfectants with a novel eukaryotic vector", Gene. 1991 Dec 15;108(2):193-9.
Proper citation: RRID:Addgene_11152 Copy
Genetic Insert: PixD
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29859829
Proper citation: RRID:Addgene_111503 Copy
Species: Aequorea victoria, Hepatitis C Virus (HCV) genotype 1a
Genetic Insert: YFP-SMASh(AI)
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCMV-SPORT6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29967496
Comments: [Ref. 1]
CHUNG H.K., JACOBS C.L., HUO Y., YANG J., KRUMM S.A., PLEMPER R.K., TSIEN R.Y. & LIN M.Z. 2015. Tunable and reversible drug control of protein production via a self-excising degron. Nat Chem Biol 11: 713-720.
[Ref. 2]
NAGAI T., IBATA K., PARK E.S., KUBOTA M., MIKOSHIBA K. & MIYAWAKI A. 2002. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol 20: 87-90.
Proper citation: RRID:Addgene_111500 Copy
Genetic Insert: PixE
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29859829
Proper citation: RRID:Addgene_111506 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cry2
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29859829
Proper citation: RRID:Addgene_111507 Copy
Genetic Insert: PixE
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29859829
Proper citation: RRID:Addgene_111505 Copy
Species: Homo sapiens
Genetic Insert: PARP1
Vector Backbone Description: Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30013063
Proper citation: RRID:Addgene_111575 Copy
Genetic Insert: cyan pre-eGRASP(p32)
Vector Backbone Description: Backbone Size:4154; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29700265
Proper citation: RRID:Addgene_111579 Copy
Species: Homo sapiens
Genetic Insert: ARH3
Vector Backbone Description: Vector Backbone:pET30; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30013063
Proper citation: RRID:Addgene_111578 Copy
Species: Synthetic
Genetic Insert: AOX
Vector Backbone Description: Backbone Marker:Markku Varjosalo, Addgene plasmid #108077; Vector Backbone:MAC-tag-C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29568061
Comments: (Gene Synthesis by GeneArt)
Proper citation: RRID:Addgene_111661 Copy
Genetic Insert: sgRNA
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:8200; Vector Backbone:pSICO derivative; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30033366
Proper citation: RRID:Addgene_111595 Copy
Species: Mus musculus
Genetic Insert: mU6-sgRNA
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:8200; Vector Backbone:pSICO derivative; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30033366
Comments: Addgene NGS results identified a V163A variant in EGFP, which does not affect plasmid function.
Proper citation: RRID:Addgene_111596 Copy
Species: Mus musculus
Genetic Insert: H2Kb
Vector Backbone Description: Backbone Marker:Addgene # 60523; Backbone Size:5661; Vector Backbone:pSBbi-pur; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_111623 Copy
Genetic Insert: Venus-LEQLE-SIINFEKL-TEW
Vector Backbone Description: Backbone Marker:Addgene # 26553; Vector Backbone:pT2/SV-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_111624 Copy
Genetic Insert: yellow pre-eGRASP(p30)
Vector Backbone Description: Backbone Size:4375; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29700265
Proper citation: RRID:Addgene_111588 Copy
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