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Species: Danio rerio
Genetic Insert: gal4/VP16
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-mRFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117778 Copy
Species: Danio rerio
Genetic Insert: dre-D145N CDK2 DN
Vector Backbone Description: Backbone Size:8500; Vector Backbone:tol2 insertion; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31451657
Proper citation: RRID:Addgene_121132 Copy
Species: Danio rerio
Genetic Insert: dre-cdk2 3'UTR mutated 199 binding
Vector Backbone Description: Backbone Size:6273; Vector Backbone:sicheck2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31451657
Proper citation: RRID:Addgene_121122 Copy
Species: Danio rerio
Genetic Insert: ApoA4a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCRII; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:25633982
Proper citation: RRID:Addgene_121902 Copy
Species: Danio rerio
Genetic Insert: ApoBa
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCRII; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:25633982
Proper citation: RRID:Addgene_121897 Copy
Species: Danio rerio
Genetic Insert: ApoBb.1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCRII; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:25633982
Proper citation: RRID:Addgene_121898 Copy
Species: Danio rerio
Genetic Insert: kank4
Vector Backbone Description: Vector Backbone:pJet1.2; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27292017
Proper citation: RRID:Addgene_121982 Copy
Species: Danio rerio
Genetic Insert: kank1a
Vector Backbone Description: Vector Backbone:pJet1.2; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27292017
Proper citation: RRID:Addgene_121978 Copy
Species: Danio rerio
Genetic Insert: kank1b
Vector Backbone Description: Vector Backbone:pJet1.2; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27292017
Proper citation: RRID:Addgene_121979 Copy
Species: Danio rerio
Genetic Insert: mfn2 gRNA
Vector Backbone Description: Backbone Marker:Tol2 kit; Backbone Size:4000; Vector Backbone:p3E; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32788232
Proper citation: RRID:Addgene_121994 Copy
Species: Danio rerio
Genetic Insert: opa1 gRNA
Vector Backbone Description: Backbone Marker:Tol2 kit; Backbone Size:4000; Vector Backbone:p3E; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32788232
Proper citation: RRID:Addgene_121995 Copy
Species: Danio rerio
Genetic Insert: alpha-bungarotoxin
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31564455
Proper citation: RRID:Addgene_122257 Copy
Species: Danio rerio
Genetic Insert: Gbait-hsp70-QF2-SV40pA
Vector Backbone Description: Vector Backbone:pGEM-T Easy; Vector Types:Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34878403
Comments: This plasmid was constructed by modifying the Gbait-hsp70:Gal4 donor plasmid described in Kimura et al. “Efficient generation of knock-in transgenic zebrafish carrying reporter/driver genes by CRISPR/Cas9-mediated genome engineering” Scientific reports vol. 4 6545. 8 Oct. 2014, doi:10.1038/srep06545. The QF2 sequence used was described in Riabinina et al. “Improved and expanded Q-system reagents for genetic manipulations” Nature methods vol. 12,3 (2015): 219-22, 5 p following 222.
Proper citation: RRID:Addgene_122563 Copy
Species: Danio rerio
Genetic Insert: Gbait-hsp70-Cre-SV40pA
Vector Backbone Description: Vector Backbone:pGEM-T Easy; Vector Types:Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34878403
Comments: This plasmid was constructed by modifying the Gbait-hsp70:Gal4 donor plasmid described in Kimura et al. “Efficient generation of knock-in transgenic zebrafish carrying reporter/driver genes by CRISPR/Cas9-mediated genome engineering” Scientific reports vol. 4 6545. 8 Oct. 2014, doi:10.1038/srep06545.
Proper citation: RRID:Addgene_122562 Copy
Species: Danio rerio
Genetic Insert: he1a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2640; Vector Backbone:pDONR P2R-P3; Vector Types:Zebrafish Danio expression; Bacterial Resistance:Kanamycin
Comments: Cloned from the construct provided by Jeff Mumm's Lab, pTol2 5xUAS:mRFP-NTRmut;HE-mCherry
Reference for the he1a promoter was first described in:
Xie et al., "Silencer-delimited transgenesis: NRSE/RE1 sequences promote neural-specific transgene expression
in a NRSF/REST-dependent manner."
BMC Biology 2012 10:93
https://doi.org/10.1186/1741-7007-10-93
Proper citation: RRID:Addgene_113881 Copy
Species: Danio rerio
Genetic Insert: he1a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2618; Vector Backbone:pDONR P2R-P3; Vector Types:Zebrafish Danio expression; Bacterial Resistance:Kanamycin
Comments: Cloned from the construct provided by Jeff Mumm's Lab, pTol2 5xUAS:YFP-p2A-NTRmutgi:HE:eYFP
Reference for the he1a promoter was first described in:
Xie et al., "Silencer-delimited transgenesis: NRSE/RE1 sequences promote neural-specific transgene expression
in a NRSF/REST-dependent manner."
BMC Biology 2012 10:93
https://doi.org/10.1186/1741-7007-10-93
Proper citation: RRID:Addgene_113879 Copy
Species: Danio rerio
Genetic Insert: tie2
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11585794
Comments: For riboprobe, digest with Eco 01091 and use T3 polymerase. The depositor noted that the tie2 Q817R mutation found in Addgene's quality control sequence does NOT affect plasmid function.
Proper citation: RRID:Addgene_13064 Copy
Species: Danio rerio
Genetic Insert: flt4
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:; Bacterial Resistance:Ampicillin
Comments: For riboprobe, digest with EcoRI and use T7 polymerase.
Proper citation: RRID:Addgene_13062 Copy
Species: Danio rerio
Genetic Insert: ephrin B2
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11585794
Comments: For riboprobe, digest with SalI and use T7 polymerase.
Proper citation: RRID:Addgene_13063 Copy
Species: Danio rerio
Genetic Insert: flk1
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11585794
Comments: For riboprobe, digest with EcoRI and use T7 polymerase.
Proper citation: RRID:Addgene_13061 Copy
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