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Species: Danio rerio
Genetic Insert: Dapper-1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Dishevelled associated protein. cloned into Cla1/Xba1 sites.
Species of Sequence Origin: zebrafish.
Notes for Use: For functional synthetic RNA cut NotI, transcribe SP6.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
Proper citation: RRID:Addgene_17082 Copy
Species: Danio rerio
Genetic Insert: Nlk1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15151990
Comments: Description: Zebrafish Nemo-like kinase 1(NLK-1), cloned into the vector CS2+. 1425bp EcoR1-Xba1 insert.
Species of Sequence Origin: zebrafish.
Notes for Use: For functional synthetic RNA, linearize with Asp 718 and transcribe with SP6. Select clones with amp.
Reference: C. J. Thorpe and R. T. Moon, Development 131:2899-2909 (2004).
Proper citation: RRID:Addgene_17075 Copy
Species: Danio rerio
Genetic Insert: Nlk1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15151990
Comments: Description: Zebrafish Nemo-like kinase 1(NLK-1), cloned into the vector CS2+. ~1425bp EcoR1-Xba1 insert.
Clone is an kinase dead mutant of NLK1, analogous to K155M mutant of mouse NLK.
Species of Sequence Origin: zebrafish.
Notes for Use: For functional synthetic RNA, linearize with Asp 718 and transcribe with SP6. Select clones with amp.
Reference: C. J. Thorpe and R. T. Moon, Development 131:2899-2909 (2004).
Proper citation: RRID:Addgene_17078 Copy
Species: Danio rerio
Genetic Insert: Dvl-2
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Dishevelled 2 deleted in amino acids 231-252, cloned into Cla/EcoR1 sites.
Notes for Use: For functional synthetic RNA cut ASP718, transcribe SP6.
Select clones with amp.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
Proper citation: RRID:Addgene_17087 Copy
Species: Danio rerio
Genetic Insert: Dvl-3
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Dishevelled 3, cloned into Cla/EcoR1 sites.
Notes for Use: For functional synthetic RNA cut NotI, transcribe SP6.
Select clones with amp.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
Proper citation: RRID:Addgene_17089 Copy
Species: Danio rerio
Genetic Insert: twhh promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCS-NBeta gal; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9314535
Comments: Insert: twhh promoter (5.2 Kb), nuclear Beta gal reporter (3 Kb)
Plasmid History:
Source: zebrafish.
Notes for Use:
Gives notochord-specific expression of Beta-gal.
Citation: Du et al., 1997, J. Cell Biology.
Proper citation: RRID:Addgene_17152 Copy
Species: Danio rerio
Genetic Insert: twhh promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:NA; Vector Types:; Bacterial Resistance:Ampicillin
Comments: Insert: twhh promoter (5.2 Kb), rabbit Beta-globin intron 2 (IVS2, 640 bp), GFP (65C, 1Kb)
Plasmid History:
Source: zebrafish.
Notes for Use:
Gives both notochord and floor-plate specific expression of GFP, owing to having the intron present.
Proper citation: RRID:Addgene_17154 Copy
Species: Danio rerio
Genetic Insert: pBS119d10L
Vector Backbone Description: Backbone Marker:Strategene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12167406
Comments: To view partial vector sequence from the depositing lab, please click on the Reviews link.
Proper citation: RRID:Addgene_22081 Copy
Species: Danio rerio
Genetic Insert: vegf
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2+; Vector Types:zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12110173
Proper citation: RRID:Addgene_22417 Copy
Species: Danio rerio
Genetic Insert: TagRFP
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2329; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Control integrates at the gene cx43.4 using the guide RNA Target "5'-GGGCCATATCTTGCCCACGA-3'" for wide-spread RFP expression in zebrafish embryos from 12hrs post injection through 5 days development
Proper citation: RRID:Addgene_117801 Copy
Species: Danio rerio
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-TagBFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117792 Copy
Species: Danio rerio
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-eGFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117791 Copy
Species: Danio rerio
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-mRFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117793 Copy
Species: Danio rerio
Genetic Insert: mRFP
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-mRFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117784 Copy
Species: Danio rerio
Genetic Insert: mRFP
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-TagBFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117786 Copy
Species: Danio rerio
Genetic Insert: gal4/VP16
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-mRFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117781 Copy
Species: Danio rerio
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-TagBFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117789 Copy
Species: Danio rerio
Genetic Insert: mRFP
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-eGFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117788 Copy
Species: Danio rerio
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-mRFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117790 Copy
Species: Danio rerio
Genetic Insert: 3x3 Stop sequence
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-eGFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117773 Copy
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