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Species: Saccharomyces cerevisiae
Genetic Insert: Med21 aa1-132 for C-terminal tagging
Vector Backbone Description: Backbone Size:2852; Vector Backbone:pMLL-KA; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Note that this plasmid needs to be grown in both Kanamycin and Ampicillin
Proper citation: RRID:Addgene_26185 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: rRNA 5'ETS ntds 923 to 1179
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pTOPO; Vector Types:Bacterial Cloning - TOPO; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:15489292
Proper citation: RRID:Addgene_23995 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 25S rRNA from +5270 to oligo y
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pTOPO; Vector Types:Bacterial Cloning - TOPO; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:15489292
Proper citation: RRID:Addgene_23996 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: rDNA 5'ETS ntds 1 to 177
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pTOPO; Vector Types:Bacterial Cloning - TOPO; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:15489292
Proper citation: RRID:Addgene_23993 Copy
Species: E.coli
Genetic Insert: KanamycinResistance-RpsL
Vector Backbone Description: Backbone Size:2935; Vector Backbone:pBluescript SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:19160076
Proper citation: RRID:Addgene_20871 Copy
Species: Gallus gallus
Genetic Insert: DISP1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3950; Vector Backbone:pCRII-TOPO; Vector Types:cDNA for in situ hybridization; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:16246323
Comments: For riboprobe cut with SpeI. Runoffs with T7 polymerase. This is not full length ORF.
Proper citation: RRID:Addgene_31235 Copy
Species: Mustela putorius furo
Genetic Insert: SLC17A7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3950; Vector Backbone:pCRII-TOPO; Vector Types:cDNA for in situ hybridization; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20575059
Comments: For riboprobe cut with NotI or EcoRI. Runoffs with SP6.
Proper citation: RRID:Addgene_31225 Copy
Species: Polyomavirus
Genetic Insert: Half genome of WU polyomavirus
Vector Backbone Description: Backbone Size:3956; Vector Backbone:pCR4-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:17480120
Proper citation: RRID:Addgene_31494 Copy
Species: polyomavirus
Genetic Insert: Half genome of WU polyomavirus
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3957; Vector Backbone:pCR4-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:17480120
Proper citation: RRID:Addgene_31493 Copy
Vector Backbone Description: Vector Backbone:pSC189; Vector Types:transposon delivery vector; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:12383515
Proper citation: RRID:Addgene_32114 Copy
Species: Staphylococcus aureus
Genetic Insert: Protein A
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:TOPO; Vector Types:Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Proper citation: RRID:Addgene_32225 Copy
Genetic Insert: QF Kan
Vector Backbone Description: Vector Backbone:pBSC5; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21857163
Comments: Please see http://www.everyvector.com/sequences/show_public/14110 for additional plasmid map.
Proper citation: RRID:Addgene_32321 Copy
Genetic Insert: EYFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR2.1 TOPO; Vector Types:Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Comments: For the addition of EYFP to the N-terminus of another cDNA by cloning the cDNA into the NotI site (of the original pCR2.1 TOPO).
Proper citation: RRID:Addgene_32438 Copy
Genetic Insert: ECFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR2.1 TOPO; Vector Types:Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Comments: For the addition of ECFP to the N-terminus of another cDNA by cloning the cDNA into the NotI site (of the original pCR2.1 TOPO).
Proper citation: RRID:Addgene_32436 Copy
Genetic Insert: EYFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR2.1 TOPO; Vector Types:Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Comments: For the addition of EYFP to the C-terminus of another cDNA by cloning the cDNA into the BamHI site (of the original pCR2.1 TOPO).
Proper citation: RRID:Addgene_32437 Copy
Species: Gallus gallus
Genetic Insert: ASIC1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCR2.1 TOPO; Vector Types:Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Proper citation: RRID:Addgene_32791 Copy
Species: Homo sapiens
Genetic Insert: ASIC1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCR2.1 TOPO; Vector Types:Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Proper citation: RRID:Addgene_32790 Copy
Species: Synthetic
Genetic Insert: dLbCas12a-VPR
Vector Backbone Description: Backbone Marker:DOI: 10.1039/C8SC02870B; Backbone Size:14777; Vector Backbone:pYFAC-riboB; Vector Types:CRISPR, Synthetic Biology, Expression in Aspergillus nidulans and related filamentous fungi.; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:32526136
Proper citation: RRID:Addgene_140197 Copy
Species: Human Merkel Cell polyomavirus
Genetic Insert: MCV replication region
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCR2.1-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:18812503
Proper citation: RRID:Addgene_28192 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: Km Resistance, neutral site II integration platform for Synechococcus
Vector Backbone Description: Vector Backbone:1572; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:10812624
Comments: NSII vector
cloning sites: NheI, XbaI, StuI, SalI, and EcoRV.
Km cassette into Klenow blunted BglII site of pAM1572. XhoI digest gives 0.7kb band. NSII vector; unique cloning sites NheI, XbaI, StuI, SalI, EcoRV.
Proper citation: RRID:Addgene_40240 Copy
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