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Species: Synthetic
Genetic Insert: T7A1, O-, O1, Lambda t1 in modified lacZ fragment
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:35188572
Comments: LacI operators separated by 400 base pairs.
Proper citation: RRID:Addgene_170474 Copy
Species: Synthetic
Genetic Insert: T7A1, Os, O-, Lambda t1 in lacZ fragment
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
Comments: LacI operators separated by 400 base pairs.
Proper citation: RRID:Addgene_170472 Copy
Species: Synthetic
Genetic Insert: T7A1, Os, O1, lambda t1, in lacZ fragment
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:28940077
Comments: LacI operators separated by 400 base pairs.
Proper citation: RRID:Addgene_170470 Copy
Species: Synthetic
Genetic Insert: T7A1, O2, O-, Lambda t1 in lacZ fragment
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
Comments: LacI operators separated by 400 base pairs.
Proper citation: RRID:Addgene_170479 Copy
Species: Mus musculus
Genetic Insert: Gq alpha
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:8863834
Comments: This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gs alpha residues (EYNLV to QYELL). This construct allows some Gs-coupled receptors to stimulate phospholipase C.
Contains an internal hemaglutinin epitope tage (DVPDYA) that has no effect on receptor coupling.
Please see supplemental document for G-protein chimera user manual.
Proper citation: RRID:Addgene_24498 Copy
Species: Rattus norvegicus
Genetic Insert: Gs alpha
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:8863834
Comments: This is Gs alpha with the C-terminal amino acids changed from Gs alpha to Gq alpha residues (QYELL to EYNLV). This construct allows some Gq-coupled receptors to stimulate Adenylate cyclase. There isn't much experience with this chimera at the moment. It may be useful for people who find the AC stimulation is better readout of receptor activation than PLC stimulation.
Contains an internal hemaglutinin epitope tage (DVPDYA) that has no effect on receptor coupling.
Please see supplemental document for G-protein chimera user manual.
Proper citation: RRID:Addgene_24499 Copy
Species: Mus musculus
Genetic Insert: Gq alpha
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:8387644
Comments: This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gi alpha residues (EYNLV to DCGLF). This construct allows many Gi-coupled receptors to stimulate phospholipase C (PLC). This is the most popular chimera, perhaps because it is easier to talk about coupling to Gi-coupled receptors with "qi5" rather than "qo5" or "qz5."
Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling.
Please see supplemental document for G-protein chimera user manual.
Proper citation: RRID:Addgene_24501 Copy
Species: Mus musculus
Genetic Insert: Gq alpha
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:8387644
Comments: This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Go alpha residues (EYNLV to GCGLY). Works the same as qi5 but (for unknown reasons) has a slightly lower basal PLC activity. This can increase the signal-to-noise ratio.
Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling.
Please see supplemental document for G-protein chimera user manual.
Proper citation: RRID:Addgene_24500 Copy
Species: Homo sapiens
Genetic Insert: RAP1
Vector Backbone Description: Vector Backbone:pAXEF-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:15469846
Comments: Q63 changed to E to give phenotype
Proper citation: RRID:Addgene_32698 Copy
Genetic Insert: Sh-CAST sgRNA 2300
Vector Backbone Description: Backbone Size:5218; Vector Backbone:pUC; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:37368499
Proper citation: RRID:Addgene_170985 Copy
Genetic Insert: mRFP
Vector Backbone Description: Backbone Size:5405; Vector Backbone:pUC; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:37368499
Proper citation: RRID:Addgene_170983 Copy
Species: Synthetic
Genetic Insert: superfolder GFP
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:6393; Vector Backbone:pTn7-SCOUT11; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:38715147
Comments: Ec100D pir+ genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir+(DHRF) Maintains plasmids at ~15 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_201001 Copy
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:6696; Vector Backbone:pTn7-SCOUT20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:38715147
Comments: Ec100D pir-116 genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir-116(DHRF) Maintains plasmids at ~250 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_200997 Copy
Species: Homo sapiens
Genetic Insert: ICAM-2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4356; Vector Backbone:pCDM8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:2497351
Comments: This clone will direct the expression of functional human ICAM-2 in a variety of cell types. This sequence has been deposited in Genbank with accession number X15606. Also see ref: Nature 329, 840-842 (1987)
Proper citation: RRID:Addgene_8634 Copy
Species: Homo sapiens
Genetic Insert: ICAM-1
Vector Backbone Description: Backbone Marker:BCCM/LMBP; Backbone Size:4356; Vector Backbone:pCDM8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:3349522
Comments: This clone will direct the expression of functional human ICAM-1 in a variety of cell types. This sequence has been deposited in Genbank with accession number J03132. Also see refs: Nature 329, 840-842 (1987) and Nature 339, 61-64 (1989)
Please use either GenBank IDs NM_000201.1 or J03132.1 as a refence sequence for the ICAM-1 insert in this plasmid. GenBank ID NM_000201.2 (updated after this plasmid was created) is not accurate for comparison.
Proper citation: RRID:Addgene_8632 Copy
Species: Homo sapiens
Genetic Insert: MAC-1 alpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4356; Vector Backbone:pCDM8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:2457584
Comments: This clone will direct the expression of functional Mac-1 upon cotransfection with the beta2 subunit in cos cells. This sequence has been deposited in Genbank with accession number J03925. Also see refs: Nature 329, 840-842 (1987) and J of Immunology 146, 648-655 (1991).
Proper citation: RRID:Addgene_8631 Copy
Vector Backbone Description: Backbone Size:4360; Vector Backbone:pBR322; Vector Types:Synthetic Biology, : Episomal vector for Phaeodactylum tricornutum; Bacterial Resistance:Ampicillin and Tetracycline
Comments: Sequence is associated with the manuscript Diner et al. 2016 in review
Proper citation: RRID:Addgene_80386 Copy
Vector Backbone Description: Backbone Size:4360; Vector Backbone:pBR322; Vector Types:Synthetic Biology, ; Episomal vector for Phaeodactylum tricornutum; Bacterial Resistance:Ampicillin and Tetracycline
Proper citation: RRID:Addgene_80387 Copy
Species: Other
Genetic Insert: ctxA
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
Comments: Kaper, J. B., J. G. Morris, Jr., and M. Nishibuchi. 1988. DNA probes for pathogenic Vibrio species, p. 65-77. In F. C. Tenover (ed.), DNA probes for infectious disease. CRC Press, Inc., Boca, Raton, Fla.
Proper citation: RRID:Addgene_63690 Copy
Genetic Insert: H5_Vietnam_1203_04
Vector Backbone Description: Backbone Size:3400; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:31189111
Proper citation: RRID:Addgene_182546 Copy
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