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Species: Synthetic
Genetic Insert: T0 terminator - Apr resistance cassette
Vector Backbone Description: Backbone Size:4948; Vector Backbone:pSH624-ssr; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:37798358
Proper citation: RRID:Addgene_198027 Copy
Species: Synthetic
Genetic Insert: gRNA scaffold - Gm resistance cassette - incP oriT
Vector Backbone Description: Backbone Size:8398; Vector Backbone:pS448∙CsR; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:37798358
Proper citation: RRID:Addgene_199240 Copy
Species: Acidaminococcus sp.
Genetic Insert: AsCas12j-2
Vector Backbone Description: Backbone Size:6888; Vector Backbone:pCRISPomyces-2; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:38672502
Comments: Please visit https://doi.org/10.1101/2021.10.28.465406 for bioRxiv preprint.
Proper citation: RRID:Addgene_191655 Copy
Species: Synthetic
Genetic Insert: Landing Pad Strain
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:35366417
Proper citation: RRID:Addgene_200007 Copy
Species: Streptomyces albidoflavus
Genetic Insert: SaindC
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pSET152; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:34489377
Proper citation: RRID:Addgene_177204 Copy
Species: Aequorea victoria
Genetic Insert: superfolder green fluorescent protein (sfGFP)
Vector Backbone Description: Vector Backbone:pSEVAb89; Vector Types:Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:40471122
Proper citation: RRID:Addgene_217505 Copy
Species: Aequorea victoria
Genetic Insert: superfolder green fluorescent protein (sfGFP)
Vector Backbone Description: Vector Backbone:pSEVAb88; Vector Types:Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:40471122
Proper citation: RRID:Addgene_217504 Copy
Species: Aequorea victoria
Genetic Insert: superfolder green fluorescent protein (sfGFP)
Vector Backbone Description: Vector Backbone:pSEVAb83; Vector Types:Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:40471122
Proper citation: RRID:Addgene_217501 Copy
Species: Aequorea victoria
Genetic Insert: superfolder green fluorescent protein (sfGFP)
Vector Backbone Description: Vector Backbone:pSEVAb82; Vector Types:Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:40471122
Proper citation: RRID:Addgene_217500 Copy
Species: Synthetic
Genetic Insert: Modified dCas9(D10A, H840A) with polyaspartate linking
Vector Backbone Description: Backbone Size:7510; Vector Backbone:pCRISPomyces-2; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:39833194
Comments: Depositor notes that there are SNPs in pSG5 Rep that cause the following amino acid substitutions: L468H R467Q C354W S215A K165E H144R, but these are not of concern for plasmid function.
Proper citation: RRID:Addgene_234429 Copy
Species: Other
Genetic Insert: Level 2 RECKLEEN plasmid
Vector Backbone Description: Vector Backbone:Custom built using Marburg Collection; Vector Types:CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:41168338
Comments: Please visit https://doi.org/10.21203/rs.3.rs-5859838/v1 for preprint.
Proper citation: RRID:Addgene_233456 Copy
Vector Backbone Description: Backbone Marker:Addgene Plasmid #226432; Vector Backbone:pCRISPRi-ccdB; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:40595632
Proper citation: RRID:Addgene_234855 Copy
Species: Synthetic
Genetic Insert: Codon optimized APOBEC1-nCas9-UGI fusion protein
Vector Backbone Description: Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:37402223
Proper citation: RRID:Addgene_209416 Copy
Species: Synthetic
Genetic Insert: codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
Vector Backbone Description: Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:40138716
Comments: Please visit https://doi.org/10.1101/2023.05.09.539971 for bioRxiv preprint.
The used type I-C CRISPR system was first described by:
Csörgő, B., León, L.M., Chau-Ly, I.J. et al. A compact Cascade–Cas3 system for targeted genome engineering. Nat Methods 17, 1183–1190 (2020). https://doi.org/10.1038/s41592-020-00980-w
Plasmid contains an IS prokaryotic transposable element that does not affect plasmid function.
The sequences used in pCRISPR-Cas3 were obtained via gene synthesis following codon optimization for S. coelicolor.
Proper citation: RRID:Addgene_209427 Copy
Species: Synthetic
Genetic Insert: Codon optimized APOBEC1-nCas9-UGI fusion protein
Vector Backbone Description: Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:38340355
Proper citation: RRID:Addgene_209448 Copy
Vector Backbone Description: Backbone Size:9396; Vector Backbone:pBAC2015, pQS-gusA, and pUC19; Vector Types:; Bacterial Resistance:Apramycin
Defining Citation: PMID:39666857
Proper citation: RRID:Addgene_227507 Copy
Species: Synthetic
Genetic Insert: AmR
Vector Backbone Description: Backbone Size:4675; Vector Backbone:pCIFR1-2; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:39778677
Proper citation: RRID:Addgene_227666 Copy
Species: Oplophorus gracilirostris
Genetic Insert: NanoLuc luciferase
Vector Backbone Description: Backbone Marker:WT; Backbone Size:33926; Vector Backbone:R388; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Proper citation: RRID:Addgene_258681 Copy
Species: Synthetic
Genetic Insert: CRISPRt transposon with gRNA expression cassette (no guide)
Vector Backbone Description: Vector Backbone:R6K_; Vector Types:Bacterial Expression, Synthetic Biology, CRISPR Associated Transposition (CAST); Bacterial Resistance:Apramycin
Comments: Please visit https://doi.org/10.1101/2024.03.01.582922 for bioRxiv preprint.
Proper citation: RRID:Addgene_225837 Copy
Species: Synthetic
Genetic Insert: CRISPRtOE transposon with gRNA expression cassette (LZ1 guide) and synthetic promoter H
Vector Backbone Description: Vector Backbone:R6K_; Vector Types:Bacterial Expression, Synthetic Biology, CRISPR Associated Transposition (CAST); Bacterial Resistance:Apramycin
Comments: Please visit https://doi.org/10.1101/2024.03.01.582922 for bioRxiv preprint.
Proper citation: RRID:Addgene_225855 Copy
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