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Species: Homo sapiens
Genetic Insert: GAlphaQ-RLuc8
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5189; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32367019
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_140982 Copy
Species: Mus musculus
Genetic Insert: PI3K catalytic subunit p110alpha-Myc- (iSH2-p85alpha)
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pSRalpha; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please note that NcoI is not a unique enzyme and will cut the plasmid DNA at five locations.
Proper citation: RRID:Addgene_1410 Copy
Species: Synthetic
Genetic Insert: SpiCee-NLS
Vector Backbone Description: Vector Backbone:pCX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32697983
Proper citation: RRID:Addgene_140900 Copy
Species: Homo sapiens
Genetic Insert: GGamma1-GFP2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5432; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32367019
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_140989 Copy
Species: Synthetic
Genetic Insert: mutSpiCee
Vector Backbone Description: Vector Backbone:pCX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32697983
Proper citation: RRID:Addgene_140903 Copy
Species: Homo sapiens
Genetic Insert: GAlpha13-RLuc8
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5203; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32367019
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_140986 Copy
Species: Homo sapiens
Genetic Insert: GGamma9-GFP2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5432; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32367019
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_140991 Copy
Species: Synthetic
Genetic Insert: TetR-sfGFP expressed with T3 promoter
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30487584
Proper citation: RRID:Addgene_140871 Copy
Species: Discosoma
Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:pMV361-strep; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:32829077
Comments: tdTomato: Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein
Shaner Nc, Campbell Re, Steinbach Pa, Giepmans Bng, Palmer Ae, Tsien Ry
(2004). Nature Biotechnology, 22(12) , 1567-1572. doi: 10.1038/nbt1037.
Proper citation: RRID:Addgene_140994 Copy
Species: Danio rerio
Genetic Insert: Rab33bb
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_140882 Copy
Species: simian virus
Genetic Insert: Large T antigen
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12781366
Proper citation: RRID:Addgene_14088 Copy
Species: Mus musculus
Genetic Insert: p19 ARF shRNA -2
Vector Backbone Description: Backbone Marker:Available from Addgene (#11795); Backbone Size:8000; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12853964
Comments: p19 ARF shRNA expressed from a lentiviral vector. The p19 ARF shRNA together with the H1 promoter was cloned from pSIRIP-puro into pLL3.7. Therefore, although the shRNA is normally expressed from the U6 promoter of pLL3.7, p19-ARF shRNA is expressed here from the H1 promoter.
Proper citation: RRID:Addgene_14091 Copy
Species: Homo sapiens
Genetic Insert: HMETTL14
Vector Backbone Description: Vector Backbone:pCDH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29290617
Proper citation: RRID:Addgene_141112 Copy
Species: Homo sapiens
Genetic Insert: HMETTL14
Vector Backbone Description: Vector Backbone:PCDH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29290617
Proper citation: RRID:Addgene_141113 Copy
Species: Homo sapiens
Genetic Insert: GCN5
Vector Backbone Description: Backbone Size:25000; Vector Backbone:pAdEasy; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16753578
Comments: Flag-GCN5 was cloned into the pAdTrack shuttle vector and adenovirus constructs were created by recombination of the shuttle vector and pAdEasy vector by electroporation into BJ5183-AD-1 bacteria (Stratagene).
AdEasy is a registered trademark of the Johns Hopkins University.
Proper citation: RRID:Addgene_14106 Copy
Vector Backbone Description: Backbone Size:6734; Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508591
Comments: Intent is to introduce low doses of pCAG FlpE to recombine a subset of cells, such that mNeon can act as an internal control to mScarlet for use in in utero electroporation or other related techniques. Genetic overexpression can be easily attached following the mScarlet through use of a mcs after the P2A.
Please visit https://www.biorxiv.org/content/10.1101/571323v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141111 Copy
Species: Homo sapiens
Genetic Insert: NFAT5
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12080349
Comments: DNA binding domain mutant, dominant negative mutant. Also called DBD5.
Proper citation: RRID:Addgene_14112 Copy
Genetic Insert: zCas9(H840A)-OsM-MLV-RT
Vector Backbone Description: Vector Backbone:pYPQ165; Vector Types:Plant Expression, CRISPR, Gateway compatible zCas9(H840A)-OsM-MLV-RT; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32222487
Comments: Protein sequence of engineered M-MLV reverse transcriptase (D200N, L603W, T306K, W313F, T330P) was obtained from published work: Anzalone, A.V., Randolph, P.B., Davis, J.R., Sousa, A.A., Koblan, L.W., Levy, J.M., Chen, P.J., Wilson, C., Newby, G.A., Raguram, A. and Liu, D.R., 2019. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature, 576(7785), pp.149-157.
Proper citation: RRID:Addgene_141080 Copy
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:31454364
Comments: Please note: Plasmid contains a T to C nucleotide mutation in attP1. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_141014 Copy
Species: S. pyogenes
Genetic Insert: Cas9 and tracrRNA
Vector Backbone Description: Vector Backbone:colE1, pAM beta; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
Defining Citation: PMID:30156038
Proper citation: RRID:Addgene_141095 Copy
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