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Species: Synthetic
Genetic Insert: human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP
Vector Backbone Description: Backbone Marker:Kleinstiver Lab (Addgene ID 139987); Vector Backbone:RTW3027; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Proper citation: RRID:Addgene_139991 Copy
Species: Synthetic
Genetic Insert: human codon optimized xCas9(3.7) with BPNLS-3xFLAG-P2A-EGFP
Vector Backbone Description: Backbone Marker:Kleinstiver Lab (Addgene ID 139987); Vector Backbone:RTW3027; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Proper citation: RRID:Addgene_139993 Copy
Species: Synthetic
Genetic Insert: human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP
Vector Backbone Description: Backbone Marker:Kleinstiver Lab (Addgene ID 139987); Vector Backbone:RTW3027; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Proper citation: RRID:Addgene_139994 Copy
Species: Synthetic
Genetic Insert: human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
Vector Backbone Description: Backbone Marker:Ketih Joung; Vector Backbone:pRZ33 ; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Proper citation: RRID:Addgene_139999 Copy
Species: Synthetic
Genetic Insert: human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
Vector Backbone Description: Backbone Marker:Kleinstiver Lab (Addgene ID 139987); Vector Backbone:RTW3027; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Proper citation: RRID:Addgene_139997 Copy
Species: Synthetic
Genetic Insert: human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
Vector Backbone Description: Backbone Marker:David Liu (Addgene Plasmid 112101); Vector Backbone:pCMV_ABEmax_P2A_GFP; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Comments: The depositing laboratory has released the equivalent ABE8e versions of this enzyme, which they have found to show much higher editing efficiency. See https://www.addgene.org/browse/article/28228617/ for the plasmids.
Proper citation: RRID:Addgene_140002 Copy
Species: Synthetic
Genetic Insert: human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
Vector Backbone Description: Backbone Marker:David Liu (Addgene Plasmid 112101); Vector Backbone:pCMV_ABEmax_P2A_GFP; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Comments: The depositing laboratory has released the equivalent ABE8e versions of this enzyme, which they have found to show much higher editing efficiency. See https://www.addgene.org/browse/article/28228617/ for the plasmids.
Proper citation: RRID:Addgene_140003 Copy
Species: Synthetic
Genetic Insert: human codon optimized CBE4max xCas9(3.7) with P2A-EGFP
Vector Backbone Description: Backbone Marker:Ketih Joung; Vector Backbone:pRZ33; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Proper citation: RRID:Addgene_140000 Copy
Species: Synthetic
Genetic Insert: pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
Vector Backbone Description: Backbone Marker:Joung Lab (DR274); Vector Backbone:DR274 (Addgene Plasmid #42250); Vector Types:Bacterial Expression, In vitro transcription (mRNA synthesis); Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_140082 Copy
Species: Synthetic
Genetic Insert: pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
Vector Backbone Description: Vector Backbone:YIplac128; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33112725
Proper citation: RRID:Addgene_140156 Copy
Species: Synthetic
Genetic Insert: pOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL
Vector Backbone Description: Vector Backbone:YIplac204; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32584255
Proper citation: RRID:Addgene_140153 Copy
Species: Synthetic
Genetic Insert: ssBip-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
Vector Backbone Description: Vector Backbone:pUASTattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33112725
Proper citation: RRID:Addgene_140166 Copy
Species: Synthetic
Genetic Insert: pSpoA-DsRed-Express2-FKBP(LV)(C22V)
Vector Backbone Description: Vector Backbone:pEarleyGate100; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33112725
Proper citation: RRID:Addgene_140169 Copy
Species: Synthetic
Genetic Insert: ssBip-DsRed-Express2-FKBP(LV)(C22V)
Vector Backbone Description: Vector Backbone:pUASTattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33112725
Proper citation: RRID:Addgene_140165 Copy
Species: Synthetic
Genetic Insert: piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)
Vector Backbone Description: Vector Backbone:pEF/myc/ER; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33112725
Comments: IMPORTANT NOTE: There is an 82-bp intron after the ER signal sequence. The signal sequence will be in frame with the final product.
Proper citation: RRID:Addgene_140160 Copy
Species: Synthetic
Genetic Insert: pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
Vector Backbone Description: Vector Backbone:pEarleyGate100; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33112725
Proper citation: RRID:Addgene_140170 Copy
Species: Synthetic
Genetic Insert: Salsa6f
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3923; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29239725
Comments: The fusion protein in this plasmid is the fluorescent genetically encoded calcium indicator Salsa6f, which is a fusion of the dimeric red fluorescent protein tdTomato, the epitope tag V5, and the green fluorescent genetically encoded calcium indicator GCaMP6f, in order from N- to C-termini. The presence of tdTomato enables visualization of transfected cells in the absence of Ca2+ signaling and concentration-independent, ratiometric readout of Ca2+ signals. Salsa6f has been introduced into transgenic mice, where its expression is under Cre recombinase control (see PMID: 29239725).
Proper citation: RRID:Addgene_140188 Copy
Species: Synthetic
Genetic Insert: dSpCas9-VPR
Vector Backbone Description: Backbone Size:3033; Vector Backbone:pGem; Vector Types:CRISPR, Synthetic Biology, Fungal genome integration.; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32526136
Comments: To be linerised with NotI for homologous recombination in Aspergillus nidulans nkuA deficient strains, selecting with glufosinate/Basta. It can also be used for random genome integration in other fungal strains.
Proper citation: RRID:Addgene_140196 Copy
Species: Synthetic
Genetic Insert: dLbCas12a-VPR
Vector Backbone Description: Backbone Marker:DOI: 10.1039/C8SC02870B; Backbone Size:14777; Vector Backbone:pYFAC-riboB; Vector Types:CRISPR, Synthetic Biology, Expression in Aspergillus nidulans and related filamentous fungi.; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:32526136
Proper citation: RRID:Addgene_140197 Copy
Species: Synthetic
Genetic Insert: CDreV
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32203389
Comments: Funded by the BRAIN Initiative.
Proper citation: RRID:Addgene_140133 Copy
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