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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pCMV-T7-SpCas9-VRER-P2A-EGFP (RTW3160) Resource Report Resource Website 1+ mentions |
RRID:Addgene_139991 | human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP | Synthetic | Ampicillin | PMID:32217751 | Backbone Marker:Kleinstiver Lab (Addgene ID 139987); Vector Backbone:RTW3027; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin | VRER=D1135V/G1218R/R1335E/T1337R | 2026-09-05 12:48:50 | 1 | |
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pCMV-T7-xCas9(3.7)-P2A-EGFP (RTW3576) Resource Report Resource Website |
RRID:Addgene_139993 | human codon optimized xCas9(3.7) with BPNLS-3xFLAG-P2A-EGFP | Synthetic | Ampicillin | PMID:32217751 | Backbone Marker:Kleinstiver Lab (Addgene ID 139987); Vector Backbone:RTW3027; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin | xCas9(3.7)=A262T/R324L/S409I/E480K/E543D/M694I/E1219V | 2026-09-05 12:48:50 | 0 | |
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pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677) Resource Report Resource Website |
RRID:Addgene_139994 | human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP | Synthetic | Ampicillin | PMID:32217751 | Backbone Marker:Kleinstiver Lab (Addgene ID 139987); Vector Backbone:RTW3027; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin | SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R | 2026-09-05 12:48:50 | 0 | |
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pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133) Resource Report Resource Website 10+ mentions |
RRID:Addgene_139999 | human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP | Synthetic | Ampicillin | PMID:32217751 | Backbone Marker:Ketih Joung; Vector Backbone:pRZ33 ; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R | 2026-09-05 12:48:50 | 13 | |
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pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008) Resource Report Resource Website 1+ mentions |
RRID:Addgene_139997 | human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP | Synthetic | Ampicillin | PMID:32217751 | Backbone Marker:Kleinstiver Lab (Addgene ID 139987); Vector Backbone:RTW3027; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin | SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R; HF1=N497A/R661A/Q695A/Q926A | 2026-09-05 12:48:50 | 1 | |
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pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562) Resource Report Resource Website 1+ mentions |
RRID:Addgene_140002 | human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP | Synthetic | Ampicillin | PMID:32217751 | The depositing laboratory has released the equivalent ABE8e versions of this enzyme, which they have found to show much higher editing efficiency. See https://www.addgene.org/browse/article/28228617/ for the plasmids. | Backbone Marker:David Liu (Addgene Plasmid 112101); Vector Backbone:pCMV_ABEmax_P2A_GFP; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin | nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R | 2026-09-05 12:48:50 | 9 |
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pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) Resource Report Resource Website 10+ mentions |
RRID:Addgene_140003 | human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP | Synthetic | Ampicillin | PMID:32217751 | The depositing laboratory has released the equivalent ABE8e versions of this enzyme, which they have found to show much higher editing efficiency. See https://www.addgene.org/browse/article/28228617/ for the plasmids. | Backbone Marker:David Liu (Addgene Plasmid 112101); Vector Backbone:pCMV_ABEmax_P2A_GFP; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin | nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R | 2026-09-05 12:48:50 | 11 |
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pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637) Resource Report Resource Website 1+ mentions |
RRID:Addgene_140000 | human codon optimized CBE4max xCas9(3.7) with P2A-EGFP | Synthetic | Ampicillin | PMID:32217751 | Backbone Marker:Ketih Joung; Vector Backbone:pRZ33; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/E543D/M694I/E1219V | 2026-09-05 12:48:50 | 1 | |
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pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485) Resource Report Resource Website 1+ mentions |
RRID:Addgene_140082 | pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485) | Synthetic | Kanamycin | Backbone Marker:Joung Lab (DR274); Vector Backbone:DR274 (Addgene Plasmid #42250); Vector Types:Bacterial Expression, In vitro transcription (mRNA synthesis); Bacterial Resistance:Kanamycin | 2026-09-05 12:48:51 | 1 | |||
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YIplac128-pOst1-ESCargo(Crimson) Resource Report Resource Website |
RRID:Addgene_140156 | pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V) | Synthetic | Ampicillin | PMID:33112725 | Vector Backbone:YIplac128; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:48:52 | 0 | ||
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YIplac204-pOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL Resource Report Resource Website |
RRID:Addgene_140153 | pOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL | Synthetic | Ampicillin | PMID:32584255 | Vector Backbone:YIplac204; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:48:51 | 0 | ||
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pUASt-ssBiP-ESCargo Resource Report Resource Website |
RRID:Addgene_140166 | ssBip-APVNTT-DsRed-Express2-FKBP(LV)(C22V) | Synthetic | Ampicillin | PMID:33112725 | Vector Backbone:pUASTattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:48:52 | 0 | ||
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pEarleyGate100-pSpoA-DsRed-Express2-FKBP(LV)(C22V) Resource Report Resource Website |
RRID:Addgene_140169 | pSpoA-DsRed-Express2-FKBP(LV)(C22V) | Synthetic | Kanamycin | PMID:33112725 | Vector Backbone:pEarleyGate100; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin | 2026-09-05 12:48:52 | 0 | ||
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pUASt-ssBiP-ESCargo* Resource Report Resource Website |
RRID:Addgene_140165 | ssBip-DsRed-Express2-FKBP(LV)(C22V) | Synthetic | Ampicillin | PMID:33112725 | Vector Backbone:pUASTattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:48:52 | 0 | ||
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pEF-pIgH-ESCargo(FTV) Resource Report Resource Website |
RRID:Addgene_140160 | piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V) | Synthetic | Ampicillin | PMID:33112725 | IMPORTANT NOTE: There is an 82-bp intron after the ER signal sequence. The signal sequence will be in frame with the final product. | Vector Backbone:pEF/myc/ER; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:48:52 | 0 | |
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pEarleyGate100-pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V) Resource Report Resource Website |
RRID:Addgene_140170 | pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V) | Synthetic | Kanamycin | PMID:33112725 | Vector Backbone:pEarleyGate100; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin | 2026-09-05 12:48:52 | 0 | ||
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Salsa6f Resource Report Resource Website 1+ mentions |
RRID:Addgene_140188 | Salsa6f | Synthetic | Kanamycin | PMID:29239725 | The fusion protein in this plasmid is the fluorescent genetically encoded calcium indicator Salsa6f, which is a fusion of the dimeric red fluorescent protein tdTomato, the epitope tag V5, and the green fluorescent genetically encoded calcium indicator GCaMP6f, in order from N- to C-termini. The presence of tdTomato enables visualization of transfected cells in the absence of Ca2+ signaling and concentration-independent, ratiometric readout of Ca2+ signals. Salsa6f has been introduced into transgenic mice, where its expression is under Cre recombinase control (see PMID: 29239725). | Backbone Marker:Clontech; Backbone Size:3923; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-09-05 12:48:52 | 1 | |
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pCRI003-pGEM-LS-Bar-PgpdA-dSpCas9-VPR-TtrpC-LS Resource Report Resource Website |
RRID:Addgene_140196 | dSpCas9-VPR | Synthetic | Ampicillin | PMID:32526136 | To be linerised with NotI for homologous recombination in Aspergillus nidulans nkuA deficient strains, selecting with glufosinate/Basta. It can also be used for random genome integration in other fungal strains. | Backbone Size:3033; Vector Backbone:pGem; Vector Types:CRISPR, Synthetic Biology, Fungal genome integration.; Bacterial Resistance:Ampicillin | 2026-09-05 12:48:52 | 0 | |
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pCRI004-pYFAC-riboB-PgpdA-dLbCas12a-VPR-TtrpC Resource Report Resource Website |
RRID:Addgene_140197 | dLbCas12a-VPR | Synthetic | Ampicillin and Kanamycin | PMID:32526136 | Backbone Marker:DOI: 10.1039/C8SC02870B; Backbone Size:14777; Vector Backbone:pYFAC-riboB; Vector Types:CRISPR, Synthetic Biology, Expression in Aspergillus nidulans and related filamentous fungi.; Bacterial Resistance:Ampicillin and Kanamycin | D832A DNase deactivated | 2026-09-05 12:48:52 | 0 | |
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pAAV-EF1a-CDreV Resource Report Resource Website |
RRID:Addgene_140133 | CDreV | Synthetic | Ampicillin | PMID:32203389 | Funded by the BRAIN Initiative. | Vector Backbone:Unknown; Vector Types:AAV; Bacterial Resistance:Ampicillin | 2026-09-05 12:48:51 | 0 |
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