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Species: Synthetic
Genetic Insert: attR1/attR2 cassette
Vector Backbone Description: Vector Backbone:pTol2005b; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73484 Copy
Species: Synthetic
Genetic Insert: Promoter 4F2 (orthogonal T7-lac variant)
Vector Backbone Description: Backbone Marker:Koffas Lab, Addgene Plasmid #49795; Backbone Size:5147; Vector Backbone:pETM6; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27079979
Comments: Constructed using degenerate site directed mutagenesis of T7-lac promoter region. Also encodes mCherry reporter.
Proper citation: RRID:Addgene_73421 Copy
Species: Synthetic
Genetic Insert: Promoter 3A2 (orthogonal T7-lac variant)
Vector Backbone Description: Backbone Marker:Koffas Lab, Addgene Plasmid #49795; Backbone Size:5147; Vector Backbone:pETM6; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27079979
Comments: Constructed using degenerate site directed mutagenesis of T7-lac promoter region. Also encodes mCherry reporter.
Proper citation: RRID:Addgene_73425 Copy
Species: Synthetic
Genetic Insert: Promoter 4A6 (orthogonal T7-lac variant)
Vector Backbone Description: Backbone Marker:Koffas Lab, Addgene Plasmid #49795; Backbone Size:5147; Vector Backbone:pETM6; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27079979
Comments: Constructed using degenerate site directed mutagenesis of T7-lac promoter region. Also encodes mCherry reporter.
Proper citation: RRID:Addgene_73424 Copy
Species: Synthetic
Genetic Insert: Promoter 1D4 (orthogonal T7-lac variant)
Vector Backbone Description: Backbone Marker:Koffas Lab, Addgene Plasmid #49795; Backbone Size:5147; Vector Backbone:pETM6; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27079979
Comments: Constructed using degenerate site directed mutagenesis of T7-lac promoter region. Also encodes mCherry reporter.
Proper citation: RRID:Addgene_73423 Copy
Species: Synthetic
Genetic Insert: Promoter 5F5 (orthogonal T7-lac variant)
Vector Backbone Description: Backbone Marker:Koffas Lab, Addgene Plasmid #49795; Backbone Size:5147; Vector Backbone:pETM6; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27079979
Comments: Constructed using degenerate site directed mutagenesis of T7-lac promoter region. Also encodes mCherry reporter.
Proper citation: RRID:Addgene_73422 Copy
Species: Synthetic
Genetic Insert: Repressor C4 (orthogonal T7-lac repressor)
Vector Backbone Description: Backbone Marker:Luciano Marraffini (Addgene plasmid # 46569); Backbone Size:9296; Vector Backbone:pdCas9; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27079979
Comments: Also encodes dCas9 and tracrRNA.
Proper citation: RRID:Addgene_73427 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:3356; Vector Backbone:pAAVS1; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26971820
Proper citation: RRID:Addgene_73500 Copy
Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Backbone Size:3356; Vector Backbone:pAAVS1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26971820
Proper citation: RRID:Addgene_73503 Copy
Species: Synthetic
Genetic Insert: LINXa3
Vector Backbone Description: Backbone Size:5940; Vector Backbone:pTriEx; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27089030
Proper citation: RRID:Addgene_73614 Copy
Species: Synthetic
Genetic Insert: lmo2 promoter:mCherry-attR1-attR2
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73489 Copy
Species: Synthetic
Genetic Insert: lmo2 promoter:EGFP-attR1-attR2
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73488 Copy
Species: Synthetic
Genetic Insert: pAzFRS.2.t1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26571098
Comments: Note: This plasmid exists as a tandem dimer approximately 12 kb in length. Please see the diagnostic digest in the Resource Information section.
Proper citation: RRID:Addgene_73546 Copy
Species: Synthetic
Genetic Insert: pAcFRS.1.t1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26571098
Proper citation: RRID:Addgene_73545 Copy
Species: Synthetic
Genetic Insert: pAcFRS.2.t1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26571098
Proper citation: RRID:Addgene_73544 Copy
Species: Synthetic
Genetic Insert: pAzFRS.1.t1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26571098
Proper citation: RRID:Addgene_73547 Copy
Species: Synthetic
Genetic Insert: fli1ep:6xMyc-attR1-attR2
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73490 Copy
Species: Synthetic
Genetic Insert: fli1ep:mCherry-attR1-attR2
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73493 Copy
Species: Synthetic
Genetic Insert: BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
Vector Backbone Description: Backbone Marker:Registry of Standardized Biological Parts; Backbone Size:2047; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27111289
Proper citation: RRID:Addgene_78691 Copy
Species: Synthetic
Genetic Insert: BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator
Vector Backbone Description: Backbone Marker:Registry of Standardized Biological Parts; Backbone Size:2047; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27111289
Proper citation: RRID:Addgene_78693 Copy
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