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Species: Synthetic
Genetic Insert: Cis_2+5
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25344638
Proper citation: RRID:Addgene_60586 Copy
Species: Synthetic
Genetic Insert: MemoryArray
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25344638
Comments: An array was constructed by concatenating the attB/P sites of the 11 phage integrases to form a linear 2kb piece of DNA. Random spacers (50 bp) with 50% GC content are included between the att sites.
Proper citation: RRID:Addgene_60585 Copy
Species: Synthetic
Genetic Insert: Cis_2+7+8+5
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25344638
Proper citation: RRID:Addgene_60589 Copy
Species: Synthetic
Genetic Insert: Cas_5+7_gfp
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25344638
Proper citation: RRID:Addgene_60595 Copy
Species: Synthetic
Genetic Insert: Spec
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25344638
Proper citation: RRID:Addgene_60593 Copy
Species: Synthetic
Genetic Insert: GalS-L
Vector Backbone Description: Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25035932
Comments: GalS-L and FruR-L also described as LLhS_Q54A and LLhF_Q60S respectively in
PLoS One. 2013 Dec 30;8(12):e83502. doi: 10.1371/journal.pone.0083502. eCollection 2013.
Rheostats and toggle switches for modulating protein function.
Meinhardt S, Manley MW Jr, Parente DJ, Swint-Kruse L.
Proper citation: RRID:Addgene_60764 Copy
Species: Synthetic
Genetic Insert: SARS-CoV Spike
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32225175
Comments: Please note that immediate availability of this material is restricted to U.S. customers only. For international customers, an export license is required. If you are interested in obtaining this plasmid, please contact [email protected]
Proper citation: RRID:Addgene_145031 Copy
Species: Synthetic
Genetic Insert: SARS-CoV-2 Spike
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32225175
Comments: See further use in:
Cell entry mechanisms of SARS-CoV-2. Jian Shang, Yushun Wan, Chuming Luo, Gang Ye, Qibin Geng, Ashley Auerbach, and Fang Li. PNAS May 26, 2020 117 (21) 11727-11734 DOI: 10.1073/pnas.2003138117
Proper citation: RRID:Addgene_145032 Copy
Species: Synthetic
Genetic Insert: 3xnls-Tq-Ca-FLITS
Vector Backbone Description: Vector Backbone:piggyBac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34887382
Comments: Read the preprint at bioRxiv: doi.org/10.1101/2021.06.21.449214
Proper citation: RRID:Addgene_145030 Copy
Species: Synthetic
Genetic Insert: RTX
Vector Backbone Description: Backbone Size:5871; Vector Backbone:modified pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please note: Addgene’s first pass of QC with Sanger sequencing was unable to cover the region containing the N210DL mutation in RTX.
Please visit https://doi.org/10.1101/2020.03.29.013342 for bioRxiv preprint.
Proper citation: RRID:Addgene_145029 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pLX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33606977
Comments: For questions regarding use of this plasmid, please contact David Root.
Please visit https://www.biorxiv.org/content/10.1101/2020.05.17.100818v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_158580 Copy
Species: Synthetic
Genetic Insert: S2*HiGlpG
Vector Backbone Description: Backbone Size:4953; Vector Backbone:PGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32795403
Proper citation: RRID:Addgene_158536 Copy
Species: Synthetic
Genetic Insert: myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain L118A - linker (GGTGGS) - Nluc
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32634377
Proper citation: RRID:Addgene_158606 Copy
Species: Synthetic
Genetic Insert: myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - p115RhoGEF RH domain - linker (GGTGGS) - Nluc
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32634377
Proper citation: RRID:Addgene_158607 Copy
Species: Synthetic
Genetic Insert: myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 I9A/W10A - linker (GGTGGS) - Nluc
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32634377
Proper citation: RRID:Addgene_158604 Copy
Species: Synthetic
Genetic Insert: myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain - linker (GGTGGS) - Nluc
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32634377
Proper citation: RRID:Addgene_158605 Copy
Species: Synthetic
Genetic Insert: myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 - linker (GGTGGS) - Nluc
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32634377
Proper citation: RRID:Addgene_158603 Copy
Species: Synthetic
Genetic Insert: PE2
Vector Backbone Description: Vector Backbone:pB-CAG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_158567 Copy
Species: Synthetic
Genetic Insert: gltA2-xylA guide array
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33460820
Comments: Read the preprint at bioRxiv: https://doi.org/10.1101/2020.07.27.222588
Proper citation: RRID:Addgene_158613 Copy
Species: Synthetic
Genetic Insert: udhA-xylA guide array
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33460820
Comments: Read the preprint at bioRxiv: https://doi.org/10.1101/2020.07.27.222588
Proper citation: RRID:Addgene_158612 Copy
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