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Species: Synthetic
Genetic Insert: YeLuc
Vector Backbone Description: Backbone Marker:DNA 2.0; Backbone Size:2707; Vector Backbone:pJ204; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25232010
Proper citation: RRID:Addgene_60119 Copy
Species: Synthetic
Genetic Insert: HyPerRed-mito
Vector Backbone Description: Backbone Size:3931; Vector Backbone:pC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25330925
Proper citation: RRID:Addgene_60247 Copy
Species: Synthetic
Genetic Insert: IMS-HyPer2
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25330925
Proper citation: RRID:Addgene_60248 Copy
Species: Synthetic
Genetic Insert: Venus-oLID-CAAX
Vector Backbone Description: Vector Backbone:pLentiLox7.0; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535392
Proper citation: RRID:Addgene_60412 Copy
Species: Synthetic
Genetic Insert: tgRFPt-SSPB
Vector Backbone Description: Vector Backbone:pLentiLox7.0; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535392
Comments: The SspB used in this construct contains two amino acid mutations that disrupt the natural dimerization of SspB. These mutations are Y11K and A15E (residue numbering from pdb code 1ou9). Here is the sequence around the mutated residues with the sites of mutation in parentheses: SSPKRP(K)LLR(E)YYDWLVDNS
Proper citation: RRID:Addgene_60415 Copy
Species: Synthetic
Genetic Insert: CaMPARI
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:5376; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25678659
Proper citation: RRID:Addgene_60421 Copy
Species: Synthetic
Genetic Insert: DAAO
Vector Backbone Description: Backbone Size:3982; Vector Backbone:pC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24020354
Proper citation: RRID:Addgene_60344 Copy
Species: Synthetic
Genetic Insert: GFPpA
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS KS+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24172903
Comments: This construct is modified pGOF18 from Young Young Il Yeom et al.,Development 122, 000-000 (1996) 881.
Proper citation: RRID:Addgene_60527 Copy
Species: Synthetic
Genetic Insert: SYFP2-mTurquoise2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22434194
Proper citation: RRID:Addgene_60493 Copy
Species: Synthetic
Genetic Insert: mTurquoise
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20081836
Proper citation: RRID:Addgene_60559 Copy
Species: Synthetic
Genetic Insert: mTurquoise
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20081836
Proper citation: RRID:Addgene_60558 Copy
Species: Synthetic
Genetic Insert: FLIM-AKAR
Vector Backbone Description: Backbone Marker:Scott Sternson; Backbone Size:5100; Vector Backbone:AAV-FLEX; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24765076
Proper citation: RRID:Addgene_60445 Copy
Species: Synthetic
Genetic Insert: Reporter_9
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25344638
Comments: This reporter plasmid contains the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). A strong constitutive promoter (BBa_J23119) is placed upstream of the attB site, which transcribes in the opposite orientation as gfp. After the integrase is expressed, the orientation of gfp is inverted and it is transcribed. After inversion, recombined attB and attP sites result in the formation of attL and attR. The attL site is located on the 5'UTR and could impact gfp expression. To insulate against this effect, we included a spacer and the ribozyme RiboJ.
Also contains mRFP in order to aid the gating of cells.
Proper citation: RRID:Addgene_60569 Copy
Species: Synthetic
Genetic Insert: Reporter_8
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25344638
Comments: This reporter plasmid contains the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). A strong constitutive promoter (BBa_J23119) is placed upstream of the attB site, which transcribes in the opposite orientation as gfp. After the integrase is expressed, the orientation of gfp is inverted and it is transcribed. After inversion, recombined attB and attP sites result in the formation of attL and attR. The attL site is located on the 5'UTR and could impact gfp expression. To insulate against this effect, we included a spacer and the ribozyme RiboJ.
Also contains mRFP in order to aid the gating of cells.
Proper citation: RRID:Addgene_60568 Copy
Species: Synthetic
Genetic Insert: FLIM-AKART391A
Vector Backbone Description: Backbone Marker:Scott Sternson; Backbone Size:5100; Vector Backbone:AAV-FLEX; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24765076
Proper citation: RRID:Addgene_60446 Copy
Species: Synthetic
Genetic Insert: Reporter_7
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25344638
Comments: This reporter plasmid contains the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). A strong constitutive promoter (BBa_J23119) is placed upstream of the attB site, which transcribes in the opposite orientation as gfp. After the integrase is expressed, the orientation of gfp is inverted and it is transcribed. After inversion, recombined attB and attP sites result in the formation of attL and attR. The attL site is located on the 5'UTR and could impact gfp expression. To insulate against this effect, we included a spacer and the ribozyme RiboJ.
Also contains mRFP in order to aid the gating of cells.
Proper citation: RRID:Addgene_60567 Copy
Species: Synthetic
Genetic Insert: Reporter_12
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25344638
Comments: This reporter plasmid contains the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). A strong constitutive promoter (BBa_J23119) is placed upstream of the attB site, which transcribes in the opposite orientation as gfp. After the integrase is expressed, the orientation of gfp is inverted and it is transcribed. After inversion, recombined attB and attP sites result in the formation of attL and attR. The attL site is located on the 5'UTR and could impact gfp expression. To insulate against this effect, we included a spacer and the ribozyme RiboJ.
Also contains mRFP in order to aid the gating of cells.
Proper citation: RRID:Addgene_60572 Copy
Species: Synthetic
Genetic Insert: Hygromycin resistant gene
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3755; Vector Backbone:pENTR2B; Vector Types:Mammalian Expression, Knock-in donor vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25434822
Proper citation: RRID:Addgene_60605 Copy
Species: Synthetic
Genetic Insert: Cis_7+8+10
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25344638
Proper citation: RRID:Addgene_60588 Copy
Species: Synthetic
Genetic Insert: Cis_7+8
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25344638
Proper citation: RRID:Addgene_60587 Copy
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