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Species: Synthetic
Genetic Insert: mito-ZifCV1.173 genetically encoded Zn(II) sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22850482
Comments: Genetically encoded, ratiometric, fluorescent biosensor targeted to the mitochondria. mito-ZifCV1.173 contains 4 tandem repeats of the first 29 aa of human cox subunit 8a (MSVLTPLLLRGLTGSARRLPVPRAKIHSL). HindIII restriction sites are at the 5' and 3' ends of the mitochondrial targeting sequence. Zn(II)-binding domain derived from mammalian Zif268. Contains truncated CFP and circularly permuted Venus 173.
Proper citation: RRID:Addgene_59010 Copy
Species: Synthetic
Genetic Insert: NLS-ZapCmR1.1 genetically encoded Zn(II) sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23173058
Comments: Genetically encoded, ratiometric, fluorescent biosensor. Contains NLS sequence at N-terminus. Zn(II)-binding domain derived from the first 2 zinc fingers of Zap 1 from Saccharomyces cerevisiae. Contains Clover and mRuby fluorescent proteins.
The Zn(II) binding domain of ZapCmR1.1 is derived from ZapCY1. The first cysteine in the first zinc finger is mutated to histidine.
Proper citation: RRID:Addgene_59011 Copy
Species: Synthetic
Genetic Insert: AAVS1-TALEN-R
Vector Backbone Description: Backbone Marker:Feng Zhang Lab; Backbone Size:7961; Vector Backbone:pTALEN; Vector Types:Mammalian Expression, AAV, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24931489
Proper citation: RRID:Addgene_59026 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:4872; Vector Backbone:pUG35; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26184437
Proper citation: RRID:Addgene_58980 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:4895; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24500276
Comments: The depositing lab has sequenced the 5' ITR, promoter, and fluorophore. Multiple digestions were done to verify the vector structure. The construct was tested in vitro and the virus was tested both in vitro and in vivo.
Proper citation: RRID:Addgene_58909 Copy
Species: Synthetic
Genetic Insert: mito-ZapCY1 high affinity Zn(II) sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22850482
Comments: Genetically encoded, ratiometric, fluorescent biosensor. mito-ZapCY1 contains 4 tandem repeats of the first 29 aa of human cox subunit 8a (MSVLTPLLLRGLTGSARRLPVPRAKIHSL). HindIII restriction sites are at the 5' and 3' ends of the mitochondrial targeting sequence. Zn(II)-binding domain derived from the first 2 zinc fingers of Zap 1 from Saccharomyces cerevisiae. Contains truncated CFP and Citrine fluorescent proteins.
Proper citation: RRID:Addgene_58996 Copy
Species: Synthetic
Genetic Insert: hSpCas9
Vector Backbone Description: Vector Backbone:PX165; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24529477
Comments: For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/.
Proper citation: RRID:Addgene_58889 Copy
Species: Synthetic
Genetic Insert: mito-ZifCV1.49 genetically encoded Zn(II) sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22850482
Comments: Genetically encoded, ratiometric, fluorescent biosensor targeted to the mitochondria. mito-ZifCV1.49 contains 4 tandem repeats of the first 29 aa of human cox subunit 8a (MSVLTPLLLRGLTGSARRLPVPRAKIHSL). HindIII restriction sites are at the 5' and 3' ends of the mitochondrial targeting sequence. Zn(II)-binding domain derived from mammalian Zif268. Contains truncated CFP and circularly permuted Venus 49.
Proper citation: RRID:Addgene_59009 Copy
Species: Synthetic
Genetic Insert: iC1C2-TS-EYFP
Vector Backbone Description: Backbone Size:5370; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24763591
Proper citation: RRID:Addgene_55630 Copy
Species: Synthetic
Genetic Insert: hChR2(H134R)-EYFP
Vector Backbone Description: Backbone Size:5600; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24908100
Proper citation: RRID:Addgene_55639 Copy
Species: Synthetic
Genetic Insert: vCre
Vector Backbone Description: Backbone Size:5331; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24908100
Proper citation: RRID:Addgene_55638 Copy
Species: Synthetic
Genetic Insert: Cre
Vector Backbone Description: Backbone Size:5331; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24908100
Proper citation: RRID:Addgene_55635 Copy
Species: Synthetic
Genetic Insert: ChR2(H134R)-EYFP
Vector Backbone Description: Backbone Size:4861; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV, Cre on/Flp on ChR2-EYFP; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24908100
Proper citation: RRID:Addgene_55644 Copy
Species: Synthetic
Genetic Insert: hChR2(H134R)-EYFP
Vector Backbone Description: Backbone Size:5600; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24908100
Proper citation: RRID:Addgene_55640 Copy
Species: Synthetic
Genetic Insert: ChR2(H134R)-EYFP
Vector Backbone Description: Backbone Size:4800; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV, Cre on/Flp off ChR2-EYFP; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24908100
Proper citation: RRID:Addgene_55646 Copy
Species: Synthetic
Genetic Insert: eYFP with introns
Vector Backbone Description: Backbone Size:4536; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV, Cre off/Flp on eYFP; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24908100
Proper citation: RRID:Addgene_55652 Copy
Species: Synthetic
Genetic Insert: eYFP with introns
Vector Backbone Description: Backbone Size:4536; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV, Cre on/Flp on eYFP; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24908100
Proper citation: RRID:Addgene_55650 Copy
Species: Synthetic
Genetic Insert: Y-GECO1.0
Vector Backbone Description: Backbone Size:3203; Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24840546
Comments: Note: Could not make stable cell line using this vector.
Proper citation: RRID:Addgene_55766 Copy
Species: Synthetic
Genetic Insert: PACR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:1700; Vector Backbone:pRSETB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24625002
Proper citation: RRID:Addgene_55775 Copy
Species: Synthetic
Genetic Insert: QuasAr2-Citrine eFRET
Vector Backbone Description: Backbone Marker:Pavel Osten; Backbone Size:9235; Vector Backbone:FCK(1.3); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25118186
Proper citation: RRID:Addgene_59172 Copy
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