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Species: Synthetic
Genetic Insert: CitrineA206K
Vector Backbone Description: Backbone Marker:Robert Gnuegge; Backbone Size:4663; Vector Backbone:pRS-derived; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25034689
Comments: For integration in yeast, linearize plasmid with PacI.
Proper citation: RRID:Addgene_58432 Copy
Species: Synthetic
Genetic Insert: CitrineA206K
Vector Backbone Description: Backbone Marker:Robert Gnuegge; Backbone Size:4663; Vector Backbone:pRS-derived; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25034689
Comments: For integration in yeast, linearize plasmid with PacI.
Proper citation: RRID:Addgene_58435 Copy
Species: Synthetic
Genetic Insert: CitrineA206K
Vector Backbone Description: Backbone Marker:Robert Gnuegge; Backbone Size:4663; Vector Backbone:pRS-derived; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25034689
Comments: For integration in yeast, linearize plasmid with PacI.
Proper citation: RRID:Addgene_58436 Copy
Species: Synthetic
Genetic Insert: PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2884; Vector Backbone:pBS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25034689
Proper citation: RRID:Addgene_58439 Copy
Species: Synthetic
Genetic Insert: LexA-ER-VP16
Vector Backbone Description: Backbone Marker:Robert Gnuegge; Backbone Size:4396; Vector Backbone:pRS-derived; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25034689
Comments: For integration in yeast, linearize plasmid with PacI.
Proper citation: RRID:Addgene_58430 Copy
Species: Synthetic
Genetic Insert: sfGFP-DocI
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5216; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25194847
Proper citation: RRID:Addgene_58708 Copy
Species: Synthetic
Genetic Insert: Spec(x2)-DocI
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5216; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25194847
Proper citation: RRID:Addgene_58713 Copy
Species: Synthetic
Genetic Insert: enhanced yellow fluorescent protein
Vector Backbone Description: Backbone Size:4401; Vector Backbone:pBI-MCS-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24611683
Proper citation: RRID:Addgene_58855 Copy
Species: Synthetic
Genetic Insert: tTA transcription factor fused to enhanced blue fluorescent protein
Vector Backbone Description: Backbone Size:5412; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24611683
Proper citation: RRID:Addgene_58854 Copy
Species: Synthetic
Genetic Insert: MESA protease chain with mCherry ectodomain and TEV protease
Vector Backbone Description: Backbone Size:5521; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24611683
Proper citation: RRID:Addgene_58868 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:4698; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16116447
Comments: Plasmid is completely sequenced by the depositing lab except parts of the origin and both ITRs. Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
Proper citation: RRID:Addgene_58867 Copy
Species: Synthetic
Genetic Insert: MESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
Vector Backbone Description: Backbone Size:5520; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24611683
Proper citation: RRID:Addgene_58869 Copy
Species: Synthetic
Genetic Insert: MESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
Vector Backbone Description: Backbone Size:5520; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24611683
Proper citation: RRID:Addgene_58875 Copy
Species: Synthetic
Genetic Insert: MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24611683
Proper citation: RRID:Addgene_58874 Copy
Species: Synthetic
Genetic Insert: MESA protease chain with FKBP rapamycin-binding ectodomain, TEV protease and mCherry fusion
Vector Backbone Description: Backbone Size:5521; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24611683
Proper citation: RRID:Addgene_58877 Copy
Species: Synthetic
Genetic Insert: MESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease
Vector Backbone Description: Backbone Size:5521; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24611683
Proper citation: RRID:Addgene_58878 Copy
Species: Synthetic
Genetic Insert: MESA protease chain with CD4 ectodomain and TEV protease
Vector Backbone Description: Backbone Size:5520; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24611683
Proper citation: RRID:Addgene_58870 Copy
Species: Synthetic
Genetic Insert: hSpCas9
Vector Backbone Description: Vector Backbone:PX165; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24529477
Comments: For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/.
Proper citation: RRID:Addgene_58888 Copy
Species: Synthetic
Genetic Insert: NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23992616
Comments: Genetically encoded, ratiometric, fluorescent biosensor. Contains nuclear exclusion signal sequence at N-terminus. Zn(II)-binding domain derived from the first 2 zinc fingers of Zap 1 from Saccharomyces cerevisiae. Contains truncated CFP and Citrine fluorescent proteins.
The Zn(II) binding domain of ZapCY2 is derived from ZapCY1. The first cysteine in each zinc finger is mutated to histidine.
Proper citation: RRID:Addgene_59016 Copy
Species: Synthetic
Genetic Insert: NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23173058
Comments: Genetically encoded, ratiometric, fluorescent biosensor. Contains nuclear exclusion signal sequence at N-terminus. Zn(II)-binding domain derived from the first 2 zinc fingers of Zap 1 from Saccharomyces cerevisiae. Contains Clover and mRuby fluorescent proteins.
The Zn(II) binding domain of ZapCmR2 is derived from ZapCmR1.1. The first cysteine in the second zinc finger is mutated to histidine.
Proper citation: RRID:Addgene_59015 Copy
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