Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25087841
Proper citation: RRID:Addgene_53024 Copy
Species: Synthetic
Genetic Insert: alpha3D
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5711; Vector Backbone:pET16b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10318910
Proper citation: RRID:Addgene_53310 Copy
Species: Synthetic
Genetic Insert: monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043132
Proper citation: RRID:Addgene_53234 Copy
Species: Synthetic
Genetic Insert: YFP
Vector Backbone Description: Backbone Marker:Lutz and Bujard 1997; Backbone Size:4000; Vector Backbone:pzs*11; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23277573
Proper citation: RRID:Addgene_53241 Copy
Species: Synthetic
Genetic Insert: SKL amino acids
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21132080
Proper citation: RRID:Addgene_53450 Copy
Species: Synthetic
Genetic Insert: Y-GECO1.0
Vector Backbone Description: Backbone Size:4563; Vector Backbone:AAV with hSyn promoter; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24840546
Proper citation: RRID:Addgene_58200 Copy
Species: Synthetic
Genetic Insert: G-CEPIA1er
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4819; Vector Backbone:pCMV/myc/ER; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24923787
Proper citation: RRID:Addgene_58215 Copy
Species: Synthetic
Genetic Insert: CEPIA2mt
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4799; Vector Backbone:pCMV/myc/mito; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24923787
Proper citation: RRID:Addgene_58218 Copy
Species: Synthetic
Genetic Insert: CEPIA4mt
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4799; Vector Backbone:pCMV/myc/mito; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24923787
Proper citation: RRID:Addgene_58220 Copy
Species: Synthetic
Genetic Insert: pea APX W41F
Vector Backbone Description: Backbone Size:2226; Vector Backbone:pZE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24803433
Comments: This plasmid encodes the W41F mutant of pea ascorbate peroxidase (APX). The W41F mutation improves enzyme activity. APX W41F is a constitutive homodimer, unlike "APEX", so APX W41F should not be used as a fusion to proteins that are sensitive to dimeric tags.
This plasmid encodes APX W41F under a constitutive promoter, and the promoter is flanked by TetR operator sites. When the plasmid is introduced into cells constitutively expressing the TetR protein, such as the GM1655 Pro cells, then the TetR protein binds to the promoter and suppresses transcription. When a tetracycline drug, such as anhydrotetracycline, is added, the TetR protein is displaced from the promoter, allowing transcription of the APX W41F gene. When the plasmid is introduced into bacterial strains that do not encode a copy of the TetR repressor, expression of APX W41F is constitutive. Any bacterial strain can be used for plasmid propagation.
The pZE plasmid system was developed by Lutz and Bujard, and described in their paper entitled "Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements" (PMID: 9092630).
Proper citation: RRID:Addgene_58199 Copy
Species: Synthetic
Genetic Insert: U6.gRNAS1 cassette
Vector Backbone Description: Backbone Size:6930; Vector Backbone:pBR322-derived pAdEasy plasmid; Vector Types:Mammalian Expression, Adenoviral, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24870050
Comments:
Proper citation: RRID:Addgene_58252 Copy
Species: Synthetic
Genetic Insert: U6.gRNAGFP cassette
Vector Backbone Description: Backbone Size:7043; Vector Backbone:pBR322-derived pAdEasy plasmid; Vector Types:Mammalian Expression, Adenoviral, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24870050
Comments:
Proper citation: RRID:Addgene_58255 Copy
Species: Synthetic
Genetic Insert: Genomic cassette
Vector Backbone Description: Vector Backbone:SEVA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:24847673
Proper citation: RRID:Addgene_58305 Copy
Species: Synthetic
Genetic Insert: Genomic cassette
Vector Backbone Description: Vector Backbone:SEVA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:24847673
Proper citation: RRID:Addgene_58304 Copy
Species: Synthetic
Genetic Insert: Genomic cassette
Vector Backbone Description: Vector Backbone:SEVA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:24847673
Proper citation: RRID:Addgene_58307 Copy
Species: Synthetic
Genetic Insert: PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2884; Vector Backbone:pBS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25034689
Proper citation: RRID:Addgene_58440 Copy
Species: Synthetic
Genetic Insert: PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2884; Vector Backbone:pBS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25034689
Proper citation: RRID:Addgene_58442 Copy
Species: Synthetic
Genetic Insert: Lifeact
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23447706
Proper citation: RRID:Addgene_58467 Copy
Species: Synthetic
Genetic Insert: Lifeact
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26317264
Proper citation: RRID:Addgene_58470 Copy
Species: Synthetic
Genetic Insert: CitrineA206K
Vector Backbone Description: Backbone Marker:Robert Gnuegge; Backbone Size:4663; Vector Backbone:pRS-derived; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25034689
Comments: For integration in yeast, linearize plasmid with PacI.
Proper citation: RRID:Addgene_58433 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the dkNET Resources search. From here you can search through a compilation of resources used by dkNET and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that dkNET has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on dkNET then you can log in from here to get additional features in dkNET such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into dkNET you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within dkNET that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
