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Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Karl-Klaus Conzelmann & Matthias Schnell; Backbone Size:13964; Vector Backbone:cSPBN (pSAD); Vector Types:Mammalian Expression, Rabies Virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23945836
Comments: Alternative plasmid name: pRVdG-1mC5E6SR
Proper citation: RRID:Addgene_52481 Copy
Species: Synthetic
Genetic Insert: TagRFP-T
Vector Backbone Description: Backbone Marker:Karl-Klaus Conzelmann & Matthias Schnell; Backbone Size:13782; Vector Backbone:cSPBN (pSAD); Vector Types:Rabies Virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22365813
Proper citation: RRID:Addgene_52489 Copy
Species: Synthetic
Genetic Insert: mTagBFP2
Vector Backbone Description: Backbone Marker:Karl-Klaus Conzelmann & Matthias Schnell; Backbone Size:13782; Vector Backbone:cSPBN (pSAD); Vector Types:Mammalian Expression, Rabies Virus; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_52490 Copy
Species: Synthetic
Genetic Insert: mTagBFP2
Vector Backbone Description: Backbone Marker:Karl-Klaus Conzelmann & Matthias Schnell; Backbone Size:13854; Vector Backbone:cSPBN (pSAD); Vector Types:Mammalian Expression, Rabies Virus; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_52498 Copy
Species: Synthetic
Genetic Insert: eNpHR 3.0-EYFP
Vector Backbone Description: Backbone Marker:Karl-Klaus Conzelmann & Matthias Schnell; Backbone Size:13782; Vector Backbone:cSPBN (pSAD); Vector Types:Mammalian Expression, Rabies Virus; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_52496 Copy
Species: Synthetic
Genetic Insert: HD NG NN NN NN HD NG HD NG HD HD HD NI NG
Vector Backbone Description: Vector Backbone:pTal4; Vector Types:TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24172903
Proper citation: RRID:Addgene_52412 Copy
Species: Synthetic
Genetic Insert: HD HD HD HD HD NI NG NG HD HD NG NI NN NI NI NN NN
Vector Backbone Description: Vector Backbone:pTal4; Vector Types:TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24172903
Proper citation: RRID:Addgene_52413 Copy
Species: Synthetic
Genetic Insert: GFP-2A-puro
Vector Backbone Description: Backbone Size:2278; Vector Backbone:minimal Amp vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24172903
Proper citation: RRID:Addgene_52380 Copy
Species: Synthetic
Genetic Insert: Fusion NLS-EGFP-P2A-Cherry-CAAX
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:5508; Vector Backbone:pcDNA3.1-(-)-Hyg; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_52421 Copy
Species: Synthetic
Genetic Insert: GFP pA
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS KS+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24172903
Comments: This construct is modified pGOF18 from Young Young Il Yeom et al.,Development 122, 000-000 (1996) 881. We have introduced neo selection cassette at the 3' end of the cloned Oct4 locus.
Please note that there are several mismatches in between Addgene's quality control and the depositor's sequence. The mismatches do NOT affect plasmid function.
Proper citation: RRID:Addgene_52382 Copy
Species: Synthetic
Genetic Insert: Right AAVS1 TALEN
Vector Backbone Description: Vector Backbone:pZT; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24833591
Comments: pZT-AAVS1-R1 TALEN was assembled using RVD monomers from Golden Gate TALEN kit 1.0 (TALEN Kit #1000000016) and a mammalian TALEN expression vector pZT.
Proper citation: RRID:Addgene_52638 Copy
Species: Synthetic
Genetic Insert: 2x FLAG-tag
Vector Backbone Description: Backbone Marker:Thermor Fisher / Fermentas; Backbone Size:2972; Vector Backbone:pJet1.2; Vector Types:blunt cloning kit; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24748663
Proper citation: RRID:Addgene_52529 Copy
Species: Synthetic
Genetic Insert: TEM-1 β-Lactamase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24817566
Proper citation: RRID:Addgene_52656 Copy
Species: Synthetic
Genetic Insert: eYFP and Blasticigin resistance cassette
Vector Backbone Description: Backbone Marker:Thermor Fisher / Fermentas; Backbone Size:2972; Vector Backbone:pJet1.2; Vector Types:blunt cloning kit; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24748663
Proper citation: RRID:Addgene_52559 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:2736; Vector Backbone:pX330; Vector Types:CRISPR, Toxoplasma gondii; Bacterial Resistance:Ampicillin
Comments: Cong L, Ran FA, Cox D, Lin S, Barretto R, et al. (2013) Multiplex genome engineering using CRISPR/Cas systems. Science 339: 819–823. doi:10.1126/science.1231143.
Proper citation: RRID:Addgene_52694 Copy
Species: Synthetic
Genetic Insert: nls-ha-MCP-VenusN-nls-ha-PCP-VenusC
Vector Backbone Description: Backbone Size:6200; Vector Backbone:phage; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24402470
Proper citation: RRID:Addgene_52985 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22618873
Proper citation: RRID:Addgene_53013 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:10130; Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25075903
Comments: Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check https://zlab.bio/ for the most up-to-date information.
Proper citation: RRID:Addgene_52962 Copy
Species: Synthetic
Genetic Insert: S. pyogenes sgRNA cassette
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25075903
Comments: Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9.
Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check https://zlab.bio/ for the most up-to-date information.
Proper citation: RRID:Addgene_52963 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25087841
Proper citation: RRID:Addgene_53018 Copy
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