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Species: Synthetic
Genetic Insert: S. pyogenes sgRNA cassette (tRNA-Tail-8A8G)
Vector Backbone Description: Vector Backbone:lentiGuide-Puro (Plasmid#52963); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32513233
Proper citation: RRID:Addgene_157984 Copy
Species: Synthetic
Genetic Insert: S. pyogenes sgRNA cassette (Tail-8A8G)
Vector Backbone Description: Vector Backbone:lentiGuide-Puro (Plasmid#52963); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32513233
Proper citation: RRID:Addgene_157981 Copy
Species: Synthetic
Genetic Insert: mGold-P2A-EBFP2
Vector Backbone Description: Backbone Size:6992; Vector Backbone:pJL2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33097540
Comments: A mammalian expression cassette was cloned into a yeast vector.
mGold: FPBaseID TBD
EBFP2: FPBaseID DVMQ7 Please note that the mVenus annotation in the plasmid map is incorrect, as this plasmid encodes mGold.
Proper citation: RRID:Addgene_157998 Copy
Species: Synthetic
Genetic Insert: mGold
Vector Backbone Description: Backbone Size:6950; Vector Backbone:pJL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33097540
Comments: Please note that the mVenus annotation in the plasmid map is incorrect, as this plasmid encodes mGold.
Proper citation: RRID:Addgene_157997 Copy
Species: Synthetic
Genetic Insert: CloGFP(1-10)
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR-SFFV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33227014
Proper citation: RRID:Addgene_157994 Copy
Species: Synthetic
Genetic Insert: pSFFV_mNG3K(1-10)
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR-SFFV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33227014
Proper citation: RRID:Addgene_157993 Copy
Species: Synthetic
Genetic Insert: mGold
Vector Backbone Description: Backbone Size:6105; Vector Backbone:pCaggs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33097540
Comments: Please note that the mVenus annotation in the plasmid map is incorrect, as this plasmid encodes mGold.
Proper citation: RRID:Addgene_157996 Copy
Species: Synthetic
Genetic Insert: Green Fluorescent Protein optimised for excitation with UV light
Vector Backbone Description: Backbone Marker:Addgene #158058; Backbone Size:8079; Vector Backbone:pDOC-K-glmS; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33226934
Proper citation: RRID:Addgene_158060 Copy
Species: Synthetic
Genetic Insert: 5'UTR synthetic stem-loop -30kcal/mol
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4716; Vector Backbone:pGL4.53(luc2/PGK); Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32846140
Proper citation: RRID:Addgene_158103 Copy
Species: Synthetic
Genetic Insert: 5'UTR synthetic stem-loop 0kcal/mol
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4716; Vector Backbone:pGL4.53(luc2/PGK); Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32846140
Proper citation: RRID:Addgene_158102 Copy
Species: Synthetic
Genetic Insert: CLYBL-CAG-DDdCas9VPH-T2A-GFP
Vector Backbone Description: Backbone Size:6069; Vector Backbone:pUCM-CLYBL-hNIL; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34031600
Proper citation: RRID:Addgene_158091 Copy
Species: Synthetic
Genetic Insert: 6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32284612
Proper citation: RRID:Addgene_158157 Copy
Species: Synthetic
Genetic Insert: QCP under the Elfa promoter and three BFP tags
Vector Backbone Description: Vector Backbone:Addgene number 75384; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33707432
Comments: Addgene NGS result found different linkers between the BFP's when compared to the backbone #75384. The depositor confirmed they observed strong BFP fluorescence and the different linkers are not a concern.
Proper citation: RRID:Addgene_158206 Copy
Species: Synthetic
Genetic Insert: 10 binding sites of MS2 without the WT sequence from oligo library experiment
Vector Backbone Description: Backbone Size:3800; Vector Backbone:CMV-SV40; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33707432
Proper citation: RRID:Addgene_158204 Copy
Species: Synthetic
Genetic Insert: 10 binding sites with high affinity to PP7 and QB coat proteins based on the machine-learning model
Vector Backbone Description: Backbone Size:3800; Vector Backbone:CMV-SV40; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33707432
Proper citation: RRID:Addgene_158203 Copy
Species: Synthetic
Genetic Insert: 10 binding sites of MS2 from oligo library experiment
Vector Backbone Description: Vector Backbone:CMV-SV40; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33707432
Proper citation: RRID:Addgene_158200 Copy
Species: Synthetic
Genetic Insert: dpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
Vector Backbone Description: Vector Backbone:pYPQ173; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34168320
Comments: Please note: This plasmid is prone to recombination in Agrobacterium. Users are recommended to use plasmid 158414
Proper citation: RRID:Addgene_158408 Copy
Species: Synthetic
Genetic Insert: dpcoCas9-TV(6xTAL-2xVP64)
Vector Backbone Description: Vector Backbone:pYPQ173; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34168320
Proper citation: RRID:Addgene_158409 Copy
Species: Synthetic
Genetic Insert: dzCas9-NG-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
Vector Backbone Description: Vector Backbone:pYPQ173; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34168320
Proper citation: RRID:Addgene_158415 Copy
Species: Synthetic
Genetic Insert: dSpRY-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
Vector Backbone Description: Vector Backbone:pYPQ173; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34168320
Proper citation: RRID:Addgene_158416 Copy
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